1- University of Agricultural Sciences and Natural Resources, Khuzestan
Abstract: (314 Views)
Introduction and Objective: In the last two decades, we have seen an increasing amount of research on the effects of nanoparticles on semen parameters. Nanoparticles are molecules with a size of 1 to 100 nm. Because of their unique properties, nanoparticles have attracted considerable attention in biotechnology, medicine, animal reproduction, and veterinary science. Zinc plays an important role in sperm viability and motility and affects protein metabolism, nucleic acid synthesis and sperm membrane stabilization. This trace element prevents the production of reactive oxygen species (ROS), defective sperms and leukocytes; It also has antioxidant effects by inhibiting lipid peroxidation and reducing the level of anti-sperm antibodies. Oxidative stress in sperm is a phenomenon that is associated with an increase in the rate of oxidation of cellular components and excessive production of reactive oxygen species. The high content of polyunsaturated fatty acids, mainly arachidonic (20:4n-6) and docosatetraenoic (22:4n-6) acids, in the phospholipids of bird sperm cell membranes renders these cells particularly susceptible to lipid peroxidation (LPO). Peroxidative damage affects sperm morphology and reduces sperm motility, and so is believed to be the main cause of the loss of fertilizing capacity observed during the storage of bird semen
Material and Methods: Sampling of 18 pieces of Bronze Turkey was done twice a week for 5 weeks. After mixing the samples with each other in order to eliminate individual effects, they were divided into 4 groups and different amounts of zinc nano oxide (0, 50, 100 and 200 µg/ml) were added to the diluent and kept at a temperature of 4 degrees Celsius. Qualitative parameters of sperm (total motility percent, progressive motility percent, viability percent, membrane integrity percent and abnormality percent) were investigated at different storage times (0, 12, 24 and 48 hours).
Results: These results were obtained using the levels of 50, 100 and 200 μg/ml of zinc nano oxide. Regarding the abnormality percen, the 100 μg/ml treatment showed the best performance during the increase in storage time, and the control treatment had the lowest performance in this respect. The best level of treatment was 100 μg/ml at the levels used and their effect on the quality of sperm. The results showed that the addition of different levels of zinc nano oxide in the turkey sperm diluent after 24 and 48 hours caused a significant difference compared to the control treatment and caused an improvement in the quality parameters of the turkey sperm compared to the control treatment.
Conclusion: The addition of zinc nano oxide improved the quality of seminal fluid and improved the qualitative parameters of turkey sperm, and adding 100 μg/ml of zinc nano oxide had the best performance among different added levels.
Material and Methods: Sampling of 18 pieces of Bronze Turkey was done twice a week for 5 weeks. After mixing the samples with each other in order to eliminate individual effects, they were divided into 4 groups and different amounts of zinc nano oxide (0, 50, 100 and 200 µg/ml) were added to the diluent and kept at a temperature of 4 degrees Celsius. Qualitative parameters of sperm (total motility percent, progressive motility percent, viability percent, membrane integrity percent and abnormality percent) were investigated at different storage times (0, 12, 24 and 48 hours).
Results: These results were obtained using the levels of 50, 100 and 200 μg/ml of zinc nano oxide. Regarding the abnormality percen, the 100 μg/ml treatment showed the best performance during the increase in storage time, and the control treatment had the lowest performance in this respect. The best level of treatment was 100 μg/ml at the levels used and their effect on the quality of sperm. The results showed that the addition of different levels of zinc nano oxide in the turkey sperm diluent after 24 and 48 hours caused a significant difference compared to the control treatment and caused an improvement in the quality parameters of the turkey sperm compared to the control treatment.
Conclusion: The addition of zinc nano oxide improved the quality of seminal fluid and improved the qualitative parameters of turkey sperm, and adding 100 μg/ml of zinc nano oxide had the best performance among different added levels.
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