1- University of Tehran
2- Urmia University
2- Urmia University
Abstract: (309 Views)
Extended Abstract
Objective
Due to the increasing demand for animal products, reproductive efficiency is considered as one of the main objectives of sheep breeding, even in cases where the main emphasis is on milk or wool production. In cases the meat production is the main breeding objective, increasing reproductive efficiency is the most important goal of breeding programs. Although increasing fertility in ewe is possible in different pathways, ovulation rate and uterine capacity are the main goals in breeding programs to achieve high fertility. Then, this study was performed to detection selection signature between Iranian native and Icelandic sheep breeds, and identify genes related to fecundity and litter size in sheep.
Materials and Methods
In this regard, the genomic information of Iranian sheep (N=154) as breeds with low litter size and Iceland breed (N=54) as one of the world high litter size sheep breeds, were used. The genomic data related to these genetic groups including Icelandic and Iranian sheep breeds were obtained from the iSheep database and the address https://disk.yandex.ru/d/3N2wEv0-9_NL0w, respectively. Data filtering and quality control, genetic differentiation index analysis (FST) and principal component analysis (PCA) were performed to determine genetic groups using PLINK 1.9 software. Ubiased FST (θ) estimator statistic were used to explore the signs of selection. The genes related to selected genomic regions was extracted using the BIOMART online database corresponding areas in the sheep genome assembly (Oar 3.1).
Results
In the identified regions as selection signatures (N=55), including 0.1% of all studied markers, the number of 391 genes were identified. Of all detected genes, at least 13 genes including BMPR2, SLC26A4, WNT16, CREB3L4, PRLR, ACVR2B, PRKCSH, HOXA9, HOXA10, Dkks, KATNAL1, OSBP2 and W5PHY6_SHEEP genes were related to reproductive performance and probably litter size in sheep. Although no significant biological pathways (with high significance) related to litter size in sheep were identified, some of the identified genes have major effects on reproductive performance. Detected genes from this study and other complementary studies about involved genes on litter size in sheep, could be effective in desigining breeding programs to improve reproductive performance.
Conclusion
Survey on identified biological pathways related to genomic differences between Iranian native and Icelandic (which is considered one of the most significant multi-twin breeds in the world) sheep breeds, was shown that, the significant biological pathways involved in immunity, smell, and hemoglobin construction. Despite of the identification a large number of genes related to The reproductive performances, these genes did not involved in a significant biological pathways. However, although no significant biological pathways (with high significance) related to litter size in sheep were identified, some of the identified genes have major effects on reproductive performance. Detected genes from this study and other complementary studies about involved genes on litter size in sheep breeds could be effective in desigining breeding programs to improve reproductive performance.
Objective
Due to the increasing demand for animal products, reproductive efficiency is considered as one of the main objectives of sheep breeding, even in cases where the main emphasis is on milk or wool production. In cases the meat production is the main breeding objective, increasing reproductive efficiency is the most important goal of breeding programs. Although increasing fertility in ewe is possible in different pathways, ovulation rate and uterine capacity are the main goals in breeding programs to achieve high fertility. Then, this study was performed to detection selection signature between Iranian native and Icelandic sheep breeds, and identify genes related to fecundity and litter size in sheep.
Materials and Methods
In this regard, the genomic information of Iranian sheep (N=154) as breeds with low litter size and Iceland breed (N=54) as one of the world high litter size sheep breeds, were used. The genomic data related to these genetic groups including Icelandic and Iranian sheep breeds were obtained from the iSheep database and the address https://disk.yandex.ru/d/3N2wEv0-9_NL0w, respectively. Data filtering and quality control, genetic differentiation index analysis (FST) and principal component analysis (PCA) were performed to determine genetic groups using PLINK 1.9 software. Ubiased FST (θ) estimator statistic were used to explore the signs of selection. The genes related to selected genomic regions was extracted using the BIOMART online database corresponding areas in the sheep genome assembly (Oar 3.1).
Results
In the identified regions as selection signatures (N=55), including 0.1% of all studied markers, the number of 391 genes were identified. Of all detected genes, at least 13 genes including BMPR2, SLC26A4, WNT16, CREB3L4, PRLR, ACVR2B, PRKCSH, HOXA9, HOXA10, Dkks, KATNAL1, OSBP2 and W5PHY6_SHEEP genes were related to reproductive performance and probably litter size in sheep. Although no significant biological pathways (with high significance) related to litter size in sheep were identified, some of the identified genes have major effects on reproductive performance. Detected genes from this study and other complementary studies about involved genes on litter size in sheep, could be effective in desigining breeding programs to improve reproductive performance.
Conclusion
Survey on identified biological pathways related to genomic differences between Iranian native and Icelandic (which is considered one of the most significant multi-twin breeds in the world) sheep breeds, was shown that, the significant biological pathways involved in immunity, smell, and hemoglobin construction. Despite of the identification a large number of genes related to The reproductive performances, these genes did not involved in a significant biological pathways. However, although no significant biological pathways (with high significance) related to litter size in sheep were identified, some of the identified genes have major effects on reproductive performance. Detected genes from this study and other complementary studies about involved genes on litter size in sheep breeds could be effective in desigining breeding programs to improve reproductive performance.
Type of Study: Research |
Subject:
ژنتیک و اصلاح نژاد دام
Received: 2024/02/3 | Accepted: 2024/09/16
Received: 2024/02/3 | Accepted: 2024/09/16
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