Volume 14, Issue 2 (8-2023)
Res Anim Prod 2023, 14(2): 61-69 |
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Bolooki Z, Jafarzadeh Shirazi M R. (2023). Changes in the Rumen Microbial Population of Estrus and Anestrus Grey Shirazi Ewes in the Estrous Cycle of the Non-Breeding Season. Res Anim Prod. 14(2), 61-69. doi:10.61186/rap.14.40.61
URL: http://rap.sanru.ac.ir/article-1-1365-en.html
URL: http://rap.sanru.ac.ir/article-1-1365-en.html
1- Department of Animal Science, Faculty of Agriculture, Shiraz University, Shiraz, Iran
Abstract: (1403 Views)
Extended Abstract
Introduction and Objective: Changes in the microbial population of the digestive tract affect the reproductive and neoral systems. Both systems are involved in the process of estrus and ovulation. In this study, the main goal wasis to investigate the changes in the rumen microbial population in non-breeding seasons during the estrous period for animals that have signs of estrus and animals that do not show estrus signs.
Material and Methods: In order to investigate changes in rumen fluid microbial population in estrus cycle, two groups of 10 animals in estrus and non estrus status were selected. Once every four days rumen fluid samples were collected for microbial culture and other investigations from both groups. In order to cultivate and separate the colonies, general culture medium for anaerobic bacteria colonies such as Plate count agar, MRS agar (de Man-Rogosa - Sharpe) and starch agar was used. Subtraction of colonies was also done by subtractive cultures or different diagnostic tests to identify colonies. Data analysis was done using SAS software version 1/9. Means were compared with the least square procedure and adjusted for Tukey's test.
Results: The comparison between the two groups at 5 different times showed that only on the day of estrus, the population of lactic acid producing bacteria was higher than anestrous group (P<0.05). The comparison between two groups of estrus and anestrus at 5 different times showed that the estrus group had a higher lipolytic bacterial population than the anestrus group in days of 5, 6 and 17 of estrus cycle (P<0.05), but at the day of 13th bacterial population in anestrus group was higher (P<0.05). The comparison between estrus and anestrus groups showed that the amount of the total bacterial population in the estrus group at all the sampling times in the estrus group was higher than anestrus group (P<0.05).
Conclusion: In total, the cultures obtained from the rumen fluid in the estrus group showed that between amylolytic, lipolytic and total colonies of anaerobic bacterial populations in certain days of estrus cycle was different between animals with sign and no sign of estrus.
Introduction and Objective: Changes in the microbial population of the digestive tract affect the reproductive and neoral systems. Both systems are involved in the process of estrus and ovulation. In this study, the main goal wasis to investigate the changes in the rumen microbial population in non-breeding seasons during the estrous period for animals that have signs of estrus and animals that do not show estrus signs.
Material and Methods: In order to investigate changes in rumen fluid microbial population in estrus cycle, two groups of 10 animals in estrus and non estrus status were selected. Once every four days rumen fluid samples were collected for microbial culture and other investigations from both groups. In order to cultivate and separate the colonies, general culture medium for anaerobic bacteria colonies such as Plate count agar, MRS agar (de Man-Rogosa - Sharpe) and starch agar was used. Subtraction of colonies was also done by subtractive cultures or different diagnostic tests to identify colonies. Data analysis was done using SAS software version 1/9. Means were compared with the least square procedure and adjusted for Tukey's test.
Results: The comparison between the two groups at 5 different times showed that only on the day of estrus, the population of lactic acid producing bacteria was higher than anestrous group (P<0.05). The comparison between two groups of estrus and anestrus at 5 different times showed that the estrus group had a higher lipolytic bacterial population than the anestrus group in days of 5, 6 and 17 of estrus cycle (P<0.05), but at the day of 13th bacterial population in anestrus group was higher (P<0.05). The comparison between estrus and anestrus groups showed that the amount of the total bacterial population in the estrus group at all the sampling times in the estrus group was higher than anestrus group (P<0.05).
Conclusion: In total, the cultures obtained from the rumen fluid in the estrus group showed that between amylolytic, lipolytic and total colonies of anaerobic bacterial populations in certain days of estrus cycle was different between animals with sign and no sign of estrus.
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