Volume 8, Issue 17 (1-2018)                   rap 2018, 8(17): 43-53 | Back to browse issues page


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(2018). The Effect of Oral Administration of Levamisole on Performance and Related Parameters with Immune System in Broiler Chickens. rap. 8(17), 43-53. doi:10.29252/rap.8.17.43
URL: http://rap.sanru.ac.ir/article-1-866-en.html
Abstract:   (3554 Views)
This experiment was conducted to study the effect of levamisole at different rearing ages on performance, some internal organ weights, white blood cells and phagocytic activity in Cobb 500 strain male broilers. The experiment was a completely randomized design, with 300 male broiler chickens in four treatments with five replicates and 15 chicks in each group. The experimental groups consisted of: 1) basal diet (without levamisole), 2) basal diet containing 3 mg levamisole in the first week; 3) basal diet containing 3 mg levamisole in the second week, and 4) basal diet containing 3 mg per kg live body weight of levamisole in the third week. Test duration was 6 weeks. Parameters examined include performance and related parameters with immune system such as number of monocytes, lymphocytes, heterophils, total protein, albumin, phagocytic activity test, antibody titers against Newcastle, influenza and Gambro disease and organs weight involved in immune system. Results showed that performance of chickens treated with levamisole at different rearing ages compared to the control group was not significant. Adding of levamisole in broiler diets increased the number of lymphocytes and heterophils at different ages of rearing (P<0.05). Adding of levamisole in the third week of rearing, increased concentrations of total protein, albumin and phagocytic activity in broilers (P<0.05). Use of levamisole in the second and third weeks of rearing broilers increased antibody titers against Gambro disease at 28 days compared to the control group (P<0.05) but with levamisole receiving group in the first week, had not significant difference. In summary, the results of this study concluded that, using three mg levamisole per kg live body weight in the first weeks of rearing period, did not improve performance, weight of organs involved in the immune system and white blood cells percent, but improved albumin, total protein and phagocytic activity in broilers.
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Type of Study: Research | Subject: Special
Received: 2018/01/10 | Accepted: 2018/01/10 | Published: 2018/01/10

References
1. Amery, W.K., F. Spreafico, .A.E Rojas, .F Denissen and M. Chingos. 1978. Adjuvant treatment with levamisole in cancer. A review of experimental and clinical data. Cancer Treatment Reviews, 4: 167-194. [DOI:10.1016/S0305-7372(77)80023-6]
2. Brousseau, P., Y. Payette and H. Tryphonas. 1999. Manual of Immunological Methods. 1st edn., CRC Press, pp: 20-45.
3. Cazella, L.N., P.E. Pardo and N. Frazatti-Gallina. 2009. Effect of levamisole on the humoral response against rabies in cattle. Veterinary Record, 165: 722-723.
4. Dair, H.F. and A.M.M. Ali. 2016. Immunomodulatory effects of levamisole hydrochloride and Nigella sativa against infectious bursal disease (IBD) in chicks. Microbiology Research International, 4(3): 17-27.
5. Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42. [DOI:10.2307/3001478]
6. Ebrahimnezhad, Y. and H. Sohrabi. 2017. The effect of different levels of levamisole drug on performance and related parameters with immune system in broiler chickens. Research on Animal Production, 8(15): 105-114 (In Persian). [DOI:10.29252/rap.8.15.105]
7. El-Kholy, H. and B.W. Kemppainen. 2005. Levamisole residues in chicken tissues and eggs. Poultry Science, 84:9-13. [DOI:10.1093/ps/84.1.9]
8. Fathi, E., M. Bozorgmehrifard, M. Ahangaran and KH. Kamali. 2009. The effect of levamisole hydrochloride on changes of lymphocytes and serum antibody against Newcastle virus in broiler chickens. Veterinary Journal, 3(7): 32-36 (In Persian).
9. Funde, S.T. 2005. Effect of Ocimum sanctum, Emblica officinalis and levamisole on immune response in immunosuppresed broilers. M.V.Sc. Thesis submitted to M.A.F.S.U., Nagpur.
10. Giambrone, J.J. and P.H. Klesius. 1985. Effect of levamisole on the response of broilers to coccidiasis vaccination on poultry. Poultry Science, 64: 1083-1089. [DOI:10.3382/ps.0641083]
11. Gomi, K., M. Morimoto and K. Nomoto. 1982. Cytotoxic T-Cell-mediated antitumor effect of levamisole against murine syngeneic fibrosarcoma. Journal of Cancer Research, 42: 4197-4202.
12. Gooi H.G. and H. Chapel.1990. Clinical immunology (a practical approach). 1st edn., Oxford University Press, pp: 51-80.
13. Gwilt, P., M. Tempero, A. Kremer, M. Connolly and C. Ding. 2000. Pharmacokinetics of levamisole in cancer patients treated with 5-fluorouracil. Cancer Chemotherapy and Pharmacology, 45: 247-251. [DOI:10.1007/s002800050036]
14. Habibi, M., M.A. Zandieh, H. Ghahri, R. Salmanzade and F. Yeganeh. 2012. Effects of levamisole on the immune response of broilers against Newcastle disease vaccines. African Journal of Pharmacy and Pharmacology, 6(25):1860-1864. [DOI:10.5897/AJPP12.294]
15. Hamidi, S., T. Shomali, R. Akbarian, A.S. Zandi and S. Sadeghi. 2016. Effect of levamisole on active antibody titres and histomorphometric parameters of immune organs in broiler chickens. Bulgarian Journal of Veterinary Medicine, DOI:10.15547/bjvm.971 (Online First). [DOI:10.15547/bjvm.971]
16. Hardman, J.G., I.E. Limberd and A.G. Gilman. 2001. The pharmacological basis of therapeutics, 10th edn., McGraw Hill, New York, 1477 pp.
17. Hersey, P. and J. Werkmeister. 1981. Inhibition of suppressor T cells in pokeweed mitogen stimulated cultures of T and B cells by levamisole in vitro and in vivo. Clinical and Experimental Immunology, 46:340-349.
18. Jin, H., Y. Li, Z. Ma, F. Zhang, Q. Xie, D. Gu and D. Wang. 2004. Effect of chemical adjuvants on DNA vaccination. Vaccine, 22:2925-2935. [DOI:10.1016/j.vaccine.2003.12.026]
19. Kang ,Y., H. Jin, G. Zheng, Q. Xie, J. Yin, Y. Yu, C. Xiao, X. Zhang, A. Chen and B. Wang. 2005. The adjuvant effect of levamisole on killed viral vaccines. Vaccine, 23: 5543-5550. [DOI:10.1016/j.vaccine.2005.07.017]
20. Katzung, B. 2001. Basic and clinical pharmacology, 8th edn., McGraw Hill, New York, 979 pp.
21. Khalegi Miran, S.N., M.A. Karimi Torshizi, M.R. Bassami and H. Jandaghi. 2010. Effect of three immunostimulants on some of indicators of broiler immune response. Japan Poultry Science, 47: 321-325. [DOI:10.2141/jpsa.009114]
22. Kulkarni, V.B., A.N. Mulbagal, V.L. Paranjape, J.B. Khot and A.V. Manda. 1973. Immunostimulating effect of tetramisole on antibody formation against Newcastle disease virus in chicks. Indian Veterinary Journal, 50: 225-227.
23. Lai, W.H., S.Y. Lu and H.L. Eng. 2002. Levamisole aids in treatment of refractory oral candidiasis in two patients with thymoma associated with myasthenia gravis: report of two cases. Chang Gung Medical Journal, 25: 606-611.
24. Maclachlan, N.J. and E.J. Dubovi. 2011. Fenner's veterinary virology. 4th edn., Elsevier Publishing Company, UK, 507 pp.
25. Mani, K., K. Sundarsan and K. Vishwanathan. 2001. Effect of immunomodulators on performance of broilers in aflatoxicosis. Indian Veterinary Journal, 78:1126-1129.
26. Mayahi, M., M.R. Seifiabad Shapouri and F. Talazadeh. 2007. Effect of levamisole administration on humoral immune response against influenza vaccine in broiler chickens. Iranian Veterinary Journal, 3(1): 89-94 (In Persian).
27. Munir, K., M.A. Muneer, A. Tiwari, E. Masaoud and R.M. Chaudhry. 2009. Effects of salinomycin on cell-mediated immunity of broiler chickens against hydro pericardium syndrome and Newcastle disease viruses. Poultry Science, 88: 86-91. [DOI:10.3382/ps.2008-00345]
28. Oladele, O.A., B.O. Emikpe, C.A.O. Adeyefa and F. Enibe. 2012. Effects of levamisole hydrochloride on cellular immune response and flock performance of commercial broilers. Brazilian Journal of Poultry Science, 14(4): 259-265. [DOI:10.1590/S1516-635X2012000400005]
29. Padmavathi, P., S.R.G. Muralidharan and S. Krishnaswamy. 1988. A study of the effect of Levamisole on Eimeria tenella experimental infection. Indian Veterinary Journal, 65: 872-874.
30. Panda, B.K. and S.K. Dwivdi. 1995. Use of some noncoccidial agents in experimentally induced coccidiosis in Japanese quails (from Hydrabad), India Journal of veterinary medicine, 15(1): 13-15.
31. Panda B.K., S.K. Sahoo and M.K. Padhi. 2004. Comparative studies on immunostimulating effect of ImmuPlus, Levamisole and Vitamins (E and C) against Ranikhet disease in broilers. Indian Journal of Poultry Science, 39(3): 252-255.
32. Porchezhian, T. and N. Punniamurthy. 2006. Effect of oral levamisole hydrochloride on feed intake and body weight of broiler chicks. Journal of Animal and Veterinary Advances, 5(10): 847-848.
33. Rajput, A.B., B.R. Kolte, J.M. Shisodiya, J.M. Chandankhede and J.M. Chahande. 2009. Effect of vitamin A, vitamin C, vitamin E and levamisole on performance of broilers. Veterinary World, 2(6): 225-227.
34. Renoux, G. 1971. Immunostimulating effect of an imidotiiazole in the immunization of mice against Brucella abortus infection. Comptes Rendus Hebdomadaires des Seances de l Academie des Sciences, 272(2): 349-350.
35. Rezayi, M. and A. Mokhtarzadeh. 2012. The effect of levamisole hydrochloride on the humoral immune response in broiler chickens vaccinated against Gambro. Proceedings of the 3rd International Veterinary Poultry Congress, February 22-23, Tehran, Iran (In Persian).
36. Roostaei Ali Mehr, M., M. Haghighian Roudsari, B. Mansori and G.R. Nikbakht Broujeni. 2012. Effects of oral levamisole hydrochloride on cellular and humoral immune responses in broiler chickens. Journal of Veterinary Research, 67(3): 235-241 (In Persian).
37. Sampson, D. and A. Lui. 1976. The effect of levamisole on cell-mediated immunity and suppressor cell function. Cancer Research, 36: 952-955.
38. SAS Institute, 2003. SAS User's Guide. Statistics. Version 9.1 edn., SAS Institute Inc., Cary, NC.
39. Shomali, T., S. Hamidi and H. Solhdoost. 2015. Levamisole improves histomorphometric parameters of small intestinal wall of broiler chickens. Bulgarian Journal of Veterinary Medicine, DOI:10. 15547/bjvm.921(Online First).
40. Snyder, D.B., W.W. Marguardt, E.T. Mallinion, E. Russeteohen, P.K. Svage and D.C. Allen. 1986. Rapid serological profiling by enzyme linked imunosorbent assay. Avian Diseases, 30: 139-148. [DOI:10.2307/1590625]
41. Soppi, E., O. Lassila, M.K. Viljanen, O.P. Lehtonen and J. Eskola. 1979. In vivo effect of levamisole on cellular and humoral immunity in normal chickens. Clinical and Experimental Immunology, 38:609-614.
42. Stevenson, H.C., I. Green, J.M. Hamilton, B.A. Calabro and D.R. Parkinson. 1991. Levamisole known effects on the immune system, clinical results and future applications to the treatment of cancer. Journal of Clinical Oncology, 9: 2052-2066. [DOI:10.1200/JCO.1991.9.11.2052]
43. Symoens, J. and M. Rosenthal. 1977. Levamisole in the modulation of the immune response: The current experimental and clinical state. Journal of the Reticuloendothelial Society, 21: 175.
44. Tekade, S.H., S.G. Mode and S.P. Waghmare. 2008. Effect of Asparagus racemosus, Sida cordifolia and levamisole on immunological parameters in experimentally induced immunosuppressed broilers. Veterinary World, 1(2): 49-50.
45. Tizard, I.R. 2012. Veterinary immunology. 9th edn., Elsevier Publishing Company, UK, 568 pp.
46. Van den Berg, T.P., N. Eterradossi, D. Toquin and G. Meulemans. 2000. Infectious bursal disease (Gumboro disease). Revue Scientifique et Technique (International Office of Epizootics), 19(2): 527-543. [DOI:10.20506/rst.19.2.1227]
47. Wauwe, J.V. and P.A.J. Janssen. 1991. On the biochemical mode of action of levamisole: an update. International Journal of Immunopharmacology, 13(1): 3-9. [DOI:10.1016/0192-0561(91)90019-4]

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