Volume 15, Issue 2 (6-2024)
Res Anim Prod 2024, 15(2): 119-130 |
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Hanai Tayran Yazd M M, Bahari Kashani R, kasraei M. (2024). Effect of Adding Organic Acids and Prebiotics on the Performance, Immune System, Histology, and Microbial Population of the Digestive Tract in Laying Hens fed with Low-Protein Percentage Soybean Meal. Res Anim Prod. 15(2), 119-130. doi:10.61186/rap.15.2.119
URL: http://rap.sanru.ac.ir/article-1-1382-en.html
URL: http://rap.sanru.ac.ir/article-1-1382-en.html
1- animal science, Islamic Azad University, Mashhad, Iran & animal science, Islamic Azad University, Mashhad, Iran
2- Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran & Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3- Biochemistry, Guilan University, Rasht, Iran & Biochemistry, Guilan University, Rasht, Iran
2- Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran & Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3- Biochemistry, Guilan University, Rasht, Iran & Biochemistry, Guilan University, Rasht, Iran
Abstract: (1217 Views)
Extended Abstract
Background: In the poultry industry, additives are used to stimulate growth, improve production performance, and improve the health of the flock. On the other hand, reducing the quality of food items and their protein level, as well as the high level of anti-nutritional substances, disturbs the balance of the microbial ecosystem of the digestive system of birds and health and ultimately reduces their performance. The health and nutritional status of poultry is largely dependent on the microbial flora of the digestive system, which directly and indirectly affects the morphology of the digestive system, nutrition, and immune system. The microbial flora of the intestine is relatively unstable and is easily affected by various nutritional factors. Therefore, this research aimed to investigate the effect of the simultaneous use of organic acids and prebiotics in diets containing low-quality soybean meal on the performance of laying hens.
Methods: This experiment was conducted using a completely randomized design in a 2 x 2 factorial arrangement using two levels of mixed organic acids and prebiotics and two types of soybean meal in the diet of laying hens. Chickens were distributed among pens based on weight and production rates for 2 weeks. Then, 192 laying hens of the LSL strain at the end of 73 weeks of age, after weighing and entering the wing number, were placed in 48 experimental cages, including four treatments with 12 replications, and fed for 12 weeks. Experimental treatments were treatment I (without the combination of organic acids and probiotics and containing 42% protein soy), treatment II (without the combination of organic acids and probiotics and containing 38% protein soy), treatment III (the combination of organic acids and probiotics and containing 42% protein soy), and treatment IV (a combination of organic acids and probiotics with 38% protein soy). To add the combination of organic acids and prebiotics, organic acid was used at a level of 1 kg per ton. The composition of the product included 30% propionic acid, 10% butyric acid, and 15% acetate plus 20% prebiotic with the origin of the Saccharomyces cerevisiae yeast cell wall. Performance traits, including production percentage and egg weight, egg mass weight, and feed conversion ratio (FCR) were measured at the end of the experiment (age 85 weeks) when all the birds were killed using the cervical vertebrae displacement method. The abdominal fat response of the immune system, histomorphology of the small intestine, and microbial population of the cecum were the investigated parameters.
Results: Except for the first week of the experiment, the addition of the combination of organic acids and probiotics and the use of high-quality soybean meal in the diet significantly increased the egg-laying percentage during the rest of the experiment (p < 0.01). In the first 4 weeks of the experiment (weeks 75-78), the percentage of egg production was under the significant influence of the interaction effects of the experimental factors. The treatment with organic acids plus prebiotics and high-quality soybean meal (treatment 3) presented the highest egg production rate (p< 0.05). From the second week to the end of the experiment, the consumption of diets containing the combination of organic acids and probiotics and high-quality soybean meal significantly increased the weight of the produced eggs (p< 0.05). The interaction effects of experimental treatments during the experimental period did not show a constant trend in the weight of produced eggs (p≥ 0.05). Adding the combination of organic acids and probiotics to the diet elevated egg mass in the entire experimental period, the difference of which was significant (p< 0.05). The use of soybean meal with low-protein soybean meal in the diet during the experimental period decreased the weight of the egg mass compared to the group consuming the diet containing high-quality soybean meal (p< 0.05). Adding the combination of organic acids and probiotics and the use of high-quality soybean meal in the diet of the studied laying hens improved the FCR during the test period (p< 0.05). The FCR in the first 5 weeks of the experiment was under the interaction effects of the experimental factors (p< 0.05). Abdominal fat at the beginning (age 73 weeks) and the end of the experiment (age 85 weeks) did not show significant differences between different treatments (p≥ 0.05). Cellular immune response to DNCB injection significantly increased using the organic acids and prebiotic mixture and decreased with the presence of low-protein soybean meal in the diet (p < 0.05). The microbial population of the cecum changed significantly under the influence of the interaction effects of the mixture of organic acids and prebiotics and the quality of the feed soup (p < 0.05). The use of a mixture of organic acids and prebiotics caused a significant increase in the height of the duodenal villi (p< 0.05). The presence of low-protein soybean meal in the diet significantly elevated the depth of crypts (p< 0.05). The ratio of villus height to crypt depth changed under the significant influence of the interaction effects of the experimental factors. The highest ratio was recorded in the treatment with a mixture of organic acids and prebiotics and high-quality soybeans in the diet while the lowest ratio was measured in the treatment without a mixture of organic acids and prebiotics and low-protein soybeans in the diet (p< 0.05). The width of the duodenal villi did not change under the influence of experimental factors, with no significant differences (p≥ 0.05).
Conclusion: In general, the results showed that using a combination of organic acids and probiotics in the diet of laying hens improved their productive performance and the health of their digestive system, as well as the performance of chickens fed with low-protein soybean meal.
Background: In the poultry industry, additives are used to stimulate growth, improve production performance, and improve the health of the flock. On the other hand, reducing the quality of food items and their protein level, as well as the high level of anti-nutritional substances, disturbs the balance of the microbial ecosystem of the digestive system of birds and health and ultimately reduces their performance. The health and nutritional status of poultry is largely dependent on the microbial flora of the digestive system, which directly and indirectly affects the morphology of the digestive system, nutrition, and immune system. The microbial flora of the intestine is relatively unstable and is easily affected by various nutritional factors. Therefore, this research aimed to investigate the effect of the simultaneous use of organic acids and prebiotics in diets containing low-quality soybean meal on the performance of laying hens.
Methods: This experiment was conducted using a completely randomized design in a 2 x 2 factorial arrangement using two levels of mixed organic acids and prebiotics and two types of soybean meal in the diet of laying hens. Chickens were distributed among pens based on weight and production rates for 2 weeks. Then, 192 laying hens of the LSL strain at the end of 73 weeks of age, after weighing and entering the wing number, were placed in 48 experimental cages, including four treatments with 12 replications, and fed for 12 weeks. Experimental treatments were treatment I (without the combination of organic acids and probiotics and containing 42% protein soy), treatment II (without the combination of organic acids and probiotics and containing 38% protein soy), treatment III (the combination of organic acids and probiotics and containing 42% protein soy), and treatment IV (a combination of organic acids and probiotics with 38% protein soy). To add the combination of organic acids and prebiotics, organic acid was used at a level of 1 kg per ton. The composition of the product included 30% propionic acid, 10% butyric acid, and 15% acetate plus 20% prebiotic with the origin of the Saccharomyces cerevisiae yeast cell wall. Performance traits, including production percentage and egg weight, egg mass weight, and feed conversion ratio (FCR) were measured at the end of the experiment (age 85 weeks) when all the birds were killed using the cervical vertebrae displacement method. The abdominal fat response of the immune system, histomorphology of the small intestine, and microbial population of the cecum were the investigated parameters.
Results: Except for the first week of the experiment, the addition of the combination of organic acids and probiotics and the use of high-quality soybean meal in the diet significantly increased the egg-laying percentage during the rest of the experiment (p < 0.01). In the first 4 weeks of the experiment (weeks 75-78), the percentage of egg production was under the significant influence of the interaction effects of the experimental factors. The treatment with organic acids plus prebiotics and high-quality soybean meal (treatment 3) presented the highest egg production rate (p< 0.05). From the second week to the end of the experiment, the consumption of diets containing the combination of organic acids and probiotics and high-quality soybean meal significantly increased the weight of the produced eggs (p< 0.05). The interaction effects of experimental treatments during the experimental period did not show a constant trend in the weight of produced eggs (p≥ 0.05). Adding the combination of organic acids and probiotics to the diet elevated egg mass in the entire experimental period, the difference of which was significant (p< 0.05). The use of soybean meal with low-protein soybean meal in the diet during the experimental period decreased the weight of the egg mass compared to the group consuming the diet containing high-quality soybean meal (p< 0.05). Adding the combination of organic acids and probiotics and the use of high-quality soybean meal in the diet of the studied laying hens improved the FCR during the test period (p< 0.05). The FCR in the first 5 weeks of the experiment was under the interaction effects of the experimental factors (p< 0.05). Abdominal fat at the beginning (age 73 weeks) and the end of the experiment (age 85 weeks) did not show significant differences between different treatments (p≥ 0.05). Cellular immune response to DNCB injection significantly increased using the organic acids and prebiotic mixture and decreased with the presence of low-protein soybean meal in the diet (p < 0.05). The microbial population of the cecum changed significantly under the influence of the interaction effects of the mixture of organic acids and prebiotics and the quality of the feed soup (p < 0.05). The use of a mixture of organic acids and prebiotics caused a significant increase in the height of the duodenal villi (p< 0.05). The presence of low-protein soybean meal in the diet significantly elevated the depth of crypts (p< 0.05). The ratio of villus height to crypt depth changed under the significant influence of the interaction effects of the experimental factors. The highest ratio was recorded in the treatment with a mixture of organic acids and prebiotics and high-quality soybeans in the diet while the lowest ratio was measured in the treatment without a mixture of organic acids and prebiotics and low-protein soybeans in the diet (p< 0.05). The width of the duodenal villi did not change under the influence of experimental factors, with no significant differences (p≥ 0.05).
Conclusion: In general, the results showed that using a combination of organic acids and probiotics in the diet of laying hens improved their productive performance and the health of their digestive system, as well as the performance of chickens fed with low-protein soybean meal.
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