Volume 14, Issue 3 (10-2023)
Res Anim Prod 2023, 14(3): 89-97 |
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AhmadFazel A, DaghighKia H, HosseinKhani A, Hamidian G, Khalilvndi Behrouzyar H. (2023). Effect of Different Sources of n-3 Unsaturated Fatty Acids on the Fatty Acid Profile and Biochemical and Physiological Characteristics of Qezel Ram Sperm. Res Anim Prod. 14(3), 89-97. doi:10.61186/rap.14.41.89
URL: http://rap.sanru.ac.ir/article-1-1358-en.html
URL: http://rap.sanru.ac.ir/article-1-1358-en.html
Asef AhmadFazel1 
, Hossein DaghighKia *1 , Ali HosseinKhani1 , Gholamreza Hamidian2 , Hamed Khalilvndi Behrouzyar3
, Hossein DaghighKia *1 , Ali HosseinKhani1 , Gholamreza Hamidian2 , Hamed Khalilvndi Behrouzyar3
1- Department of Animal Science, Faculty of Agriculture, Tabriz University, Tabriz, Iran
2- Department of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz University, Tabriz, Iran
3- Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
2- Department of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz University, Tabriz, Iran
3- Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
Abstract: (1643 Views)
Extended Abstract
Introduction and Objectives: Recent studies have shown that consuming different sources of omega-3 fatty acids can play an important role in improving sperm quality in different species. The purpose of this study was to investigate the effects of using different sources of omega-3 fatty acids in the diet of Qezel ram, on volume and concentration of semen, fatty acid profile and characteristics of fresh and frozen-thawed sperm.
Materials and methods: In this study, which was carried out for three months, 16 adult rams of Qezel breed were selected and randomly divided into 4 groups, and each group received one of the diets, all of which were isocaloric and isonitrogenous. The first group: diet without oil (control), the second group: diet with palm oil, the third group: diet with flax oil and the fourth group: diet with fish oil. After habituation of the rams, sperm collection was done weekly with an artificial vagina for 13 weeks. After evaluating the appearance and measuring the sperm volume, sperm concentration and motility in the fresh sperm samples, the sperm samples were frozen packing in straws. Physiological and biochemical characteristics of sperms after freezing-thawing, including sperm motility parameters (CASA), viability percentage, HOST and Hancock test and fatty acid profile were evaluated.
Results: The results showed that the concentration, viability and integrity of sperm plasma membrane in the third and fourth groups were significantly increased compared to the control and second groups (P<0.01). Also, the abnormal morphology in the third and fourth groups was significantly reduced compared to the control and palm oil receiving groups (P<0.01). Motility parameters such as total motility, straight line velocity (VSL), curvilinear velocity (VCL) and average path velocity (VAP) in the third and fourth groups showed a significant increase compared to the control and the second group (P<0.05). Also, there was a significant increase in progressive motility in the fourth group compared to the first and second groups, and in the third group compared to the second group (P<0.05). Diet containing palm oil caused a significant increase in sperm palmitic acid in week 12 (P<0.05). Eicosapentaenoic and docosahexaenoic fatty acids increased significantly in the groups receiving fish oil and flax compared to the group receiving palm oil and control (without oil) (P<0.01). The rest of the measured fatty acids did not show any significant changes.
Conclusion: The results showed that adding fish and flax oil to the diet of Qezel rams for 3 months improves the quality of physico-chemical characteristics and improves the freezing ability of sperm.
Introduction and Objectives: Recent studies have shown that consuming different sources of omega-3 fatty acids can play an important role in improving sperm quality in different species. The purpose of this study was to investigate the effects of using different sources of omega-3 fatty acids in the diet of Qezel ram, on volume and concentration of semen, fatty acid profile and characteristics of fresh and frozen-thawed sperm.
Materials and methods: In this study, which was carried out for three months, 16 adult rams of Qezel breed were selected and randomly divided into 4 groups, and each group received one of the diets, all of which were isocaloric and isonitrogenous. The first group: diet without oil (control), the second group: diet with palm oil, the third group: diet with flax oil and the fourth group: diet with fish oil. After habituation of the rams, sperm collection was done weekly with an artificial vagina for 13 weeks. After evaluating the appearance and measuring the sperm volume, sperm concentration and motility in the fresh sperm samples, the sperm samples were frozen packing in straws. Physiological and biochemical characteristics of sperms after freezing-thawing, including sperm motility parameters (CASA), viability percentage, HOST and Hancock test and fatty acid profile were evaluated.
Results: The results showed that the concentration, viability and integrity of sperm plasma membrane in the third and fourth groups were significantly increased compared to the control and second groups (P<0.01). Also, the abnormal morphology in the third and fourth groups was significantly reduced compared to the control and palm oil receiving groups (P<0.01). Motility parameters such as total motility, straight line velocity (VSL), curvilinear velocity (VCL) and average path velocity (VAP) in the third and fourth groups showed a significant increase compared to the control and the second group (P<0.05). Also, there was a significant increase in progressive motility in the fourth group compared to the first and second groups, and in the third group compared to the second group (P<0.05). Diet containing palm oil caused a significant increase in sperm palmitic acid in week 12 (P<0.05). Eicosapentaenoic and docosahexaenoic fatty acids increased significantly in the groups receiving fish oil and flax compared to the group receiving palm oil and control (without oil) (P<0.01). The rest of the measured fatty acids did not show any significant changes.
Conclusion: The results showed that adding fish and flax oil to the diet of Qezel rams for 3 months improves the quality of physico-chemical characteristics and improves the freezing ability of sperm.
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