Volume 14, Issue 2 (8-2023)
Res Anim Prod 2023, 14(2): 44-51 |
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Taheri Nasab M, Hashemi S R, Ramzanpour S S, Rostami S, Arabiyan E. (2023). Evaluation of Metallothionein Gene as a Biomarker in the Liver of Broilers Fed Silver Nanoparticles Coated on Zeolite in Heat Stress Conditions. Res Anim Prod. 14(2), 44-51. doi:10.61186/rap.14.40.44
URL: http://rap.sanru.ac.ir/article-1-1269-en.html
URL: http://rap.sanru.ac.ir/article-1-1269-en.html
Mahmoud Taheri Nasab *1 
, Seyed Reza Hashemi1 , Seyedeh Sanaz Ramzanpour1 , Sharif Rostami1 , Elnaz Arabiyan1
, Seyed Reza Hashemi1 , Seyedeh Sanaz Ramzanpour1 , Sharif Rostami1 , Elnaz Arabiyan1
1- Gorgan University of Agricultural Sciences and Natural Recources
Abstract: (1633 Views)
Extended Abstract
Introduction and Objective: Due to the ban on the use of antibiotics as a growth promoter in the broiler industry since 2007, researchers are always looking for a suitable alternative to antibiotics. Silver nanoparticles with antibacterial, antifungal and antiviral properties were used as one of the proposed alternatives to antibiotics and the purpose of this study was to evaluate the effect of coated silver nanoparticles on zeolite on the expression of metallothionein gene as a biomarker of heavy metals in the liver cells of broiler chickens.
Materials and methods: In this experiment, Ross 308 broiler chickens were allocated to five experimental diets: control or basal diet, basal diet supplemented by 1% zeolite, basal diet supplemented by 1% zeolite coated with 0.5% nanosilver, basal diet supplemented by 0.15% organic acid and basal diet supplemented by 1% zeolite coated with 0.5% nanosilver and 0.15% organic acid were designed under normal and heat stress conditions. Liver tissue samples were collected on days 21 and 42 of rearing period. In order to perform gene expression studies, RNA was extracted and its quality was evaluated by electrophoresis and after purification, cDNA synthesis was performed. In the following the polymerase chain reaction was performed for tissue cDNA samples for metallothionein gene and also betaactin as reference gene.
Results: The results showed a significant increase in expression of metallothionein gene in the liver tissue of broilers in zeolite treatment on d 21 (p<0.05). Also, on d 42 of rearing period without heat stress conditions, a significant increase in metallothionein gene expression were observed on silver nanoparticles coated on zeolite and 0.15% organic acid (NSOA) treatment. Also, on d 42 under heat stress conditions the results showed a significant increase in zeolite (Z) and silver nanoparticles coated on zeolite (NS) treatments in liver tissue in comparison with control treatment (p<0.05).
Conclusion: The results of present study indicated that metallothionein gene could be considered as a reliable heavy metal biomarker under normal and heat stress conditions in broiler chickens.
Introduction and Objective: Due to the ban on the use of antibiotics as a growth promoter in the broiler industry since 2007, researchers are always looking for a suitable alternative to antibiotics. Silver nanoparticles with antibacterial, antifungal and antiviral properties were used as one of the proposed alternatives to antibiotics and the purpose of this study was to evaluate the effect of coated silver nanoparticles on zeolite on the expression of metallothionein gene as a biomarker of heavy metals in the liver cells of broiler chickens.
Materials and methods: In this experiment, Ross 308 broiler chickens were allocated to five experimental diets: control or basal diet, basal diet supplemented by 1% zeolite, basal diet supplemented by 1% zeolite coated with 0.5% nanosilver, basal diet supplemented by 0.15% organic acid and basal diet supplemented by 1% zeolite coated with 0.5% nanosilver and 0.15% organic acid were designed under normal and heat stress conditions. Liver tissue samples were collected on days 21 and 42 of rearing period. In order to perform gene expression studies, RNA was extracted and its quality was evaluated by electrophoresis and after purification, cDNA synthesis was performed. In the following the polymerase chain reaction was performed for tissue cDNA samples for metallothionein gene and also betaactin as reference gene.
Results: The results showed a significant increase in expression of metallothionein gene in the liver tissue of broilers in zeolite treatment on d 21 (p<0.05). Also, on d 42 of rearing period without heat stress conditions, a significant increase in metallothionein gene expression were observed on silver nanoparticles coated on zeolite and 0.15% organic acid (NSOA) treatment. Also, on d 42 under heat stress conditions the results showed a significant increase in zeolite (Z) and silver nanoparticles coated on zeolite (NS) treatments in liver tissue in comparison with control treatment (p<0.05).
Conclusion: The results of present study indicated that metallothionein gene could be considered as a reliable heavy metal biomarker under normal and heat stress conditions in broiler chickens.
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