Volume 12, Issue 34 (12-2021)                   rap 2021, 12(34): 78-88 | Back to browse issues page

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Yekani V, khalilvandi-behroozyar H, Pirmohammadi R, Donyadoost chalan M, mohtashami B. Production and Evaluation of Bioactive Peptides Resulting from Hydrolysis of Chicken Meat Waste and Whey Powder Through Autoclave and Bio-Fermentation Process. rap. 2021; 12 (34) :78-88
URL: http://rap.sanru.ac.ir/article-1-1179-en.html
Urmia University
Abstract:   (493 Views)
Extended Abstract
Introduction and Objective: Chicken meat waste and whey powder as by-products are often rich in high quality proteins and can be hydrolyzed by protease enzymes produced by microorganisms. The aim of this study was to produce bioactive peptides from chicken meat waste and whey powder by bio-fermentation by Bacillus subtilis, Bacillus licheniformis, Aspergillus oryzae, and hydrolysis by autoclave. Also, isolated and hydrolyzed protein sources were evaluated under in vitro condition.
Material and Methods: Chicken meat wastes were first degreased and then their protein was isolated through alkaline extraction and acid precipitation. The microorganisms were cultured in a specific culture medium and then added to the protein isolated in the previous step, and the fermentation hydrolysis process was performed. An autoclave was also used for hydrolysis. Isolated and hydrolyzed protein sources were evaluated in vitro to determine the amounts of crude protein, organic matter, ash, dry matter, ether extract, and the amount of gas produced in vitro. Finally, the data obtained from the laboratory evaluation were statistically analyzed using a completely randomized statistical model using SAS 9.4 software
Results: The results of production and evaluation of isolated and hydrolyzed protein sources showed that during hydrolysis, minor changes were made in the chemical composition, but the production of low molecular weight peptides increased. Also, it was found that the production of bioactive peptides from different sources by different methods has different efficiencies. Among the products of chicken meat waste and whey powder, the highest amount of low molecular weight peptides was produced by Bacillus subtilis (1.227 and 0.786 μg / ml, respectively). Autoclave treatment also led to the breaking of weak chemical bonds and increased production of small peptides. Also, the results of in vitro gas production method showed that hydrolysis of chicken meat waste and whey powder using Oryzae, increased the amount of gas produced (139.77 and 189.21, respectively) and all hydrolysis treatments improved the digestibility of dry mater.
Conclusion: The general results of the study showed that hydrolysis of chicken meat waste and whey powder through bio-fermentation and hydrolysis by autoclave increased the production of bioactive peptides and Bacillus subtilis produced the highest amount of peptides. Also, Aspergillus oryzea treatment compared to other treatments made the largest increase in the amount of gas production and fermentable part, which shows the positive effect of hydrolyzed protein production by this method in increasing the biological activity of chicken meat waste and whey protein.
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Type of Study: Research | Subject: تغذیه نشخوارکنندگان
Received: 2021/02/7 | Revised: 2022/02/5 | Accepted: 2021/06/12 | Published: 2022/02/2

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