Volume 13, Issue 35 (3-2022)
rap 2022, 13(35): 124-129 |
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Shakibamehr N, Harzandi N, Mosavari N, Mojgani N. (2022). Serological Evaluation of Horse Specimens Suspected of Glanders using Western Blot. rap. 13(35), 124-129. doi:10.52547/rap.13.35.124
URL: http://rap.sanru.ac.ir/article-1-1155-en.html
URL: http://rap.sanru.ac.ir/article-1-1155-en.html
Islamic Azad University
Abstract: (1422 Views)
Extended Abstract
Introduction and Objective: Burkholderia mallei, is the etiologic agent of the glanders disease. Clinical and bacteriological diagnosis of glanders is difficult in the early stages of the disease. Some methods such as Complement fixation test (CFT) due to false positive results and troublesome for veterinary authorities and cause financial losses to animal owners, The other one is Malleination test, which requires appropriate equipment and efficient laboratory personnel. Therefore, in order to quickly and accurately diagnose the disease, especially in areas that cannot be kept animals, new methods should be used to identify the disease. The Western blot is a serological diagnostic test and has been recommended by the World Organization for Animal Health (OIE). In this study, a Western blot assay making use of a purified lipopoly-saccharide (LPS) containing antigen of Burkholderia mallei was designated.
Material and Methods: in this study, a total of 75 sera were collected from different horse populations from several geographical areas of Iran. Specificity and sensitivity of the Complement fixation test, ELISA and a Western blot were compared for serodiagnosis of glanders. ELISA test was based on B. mallei antigens and Western blot made use of a purified LPS-containing B. mallei-antigen.
Results: The Western blot and ELISA, were more specific than the Complement fixation test. ELISA based on B. mallei antigens had more sensitivity compared to Complement fixation test and Western blot. Finally, sensitivity and specificity were obtained for Complement fixation test (92.31% and 98.38%), Western blot (92.31%, 100%) and (100% and 100%) respectively.
Conclusion: Complement fixation test for glanders is still the prescribed serological method for commercial purposes, which is used to confirm the health of animals. However, in order to maintain the biosafety of valuable and expensive horse colonies, it is important to implement a more accurate and intelligent disease control and eradication program. This enhances the laboratory diagnostic capabilities to better understand the cause of the disease and to use and optimize the diagnostic methods of glanders in the country. Therefore, efforts to further improve and optimize ELISA and Western blotting should be continue.
Introduction and Objective: Burkholderia mallei, is the etiologic agent of the glanders disease. Clinical and bacteriological diagnosis of glanders is difficult in the early stages of the disease. Some methods such as Complement fixation test (CFT) due to false positive results and troublesome for veterinary authorities and cause financial losses to animal owners, The other one is Malleination test, which requires appropriate equipment and efficient laboratory personnel. Therefore, in order to quickly and accurately diagnose the disease, especially in areas that cannot be kept animals, new methods should be used to identify the disease. The Western blot is a serological diagnostic test and has been recommended by the World Organization for Animal Health (OIE). In this study, a Western blot assay making use of a purified lipopoly-saccharide (LPS) containing antigen of Burkholderia mallei was designated.
Material and Methods: in this study, a total of 75 sera were collected from different horse populations from several geographical areas of Iran. Specificity and sensitivity of the Complement fixation test, ELISA and a Western blot were compared for serodiagnosis of glanders. ELISA test was based on B. mallei antigens and Western blot made use of a purified LPS-containing B. mallei-antigen.
Results: The Western blot and ELISA, were more specific than the Complement fixation test. ELISA based on B. mallei antigens had more sensitivity compared to Complement fixation test and Western blot. Finally, sensitivity and specificity were obtained for Complement fixation test (92.31% and 98.38%), Western blot (92.31%, 100%) and (100% and 100%) respectively.
Conclusion: Complement fixation test for glanders is still the prescribed serological method for commercial purposes, which is used to confirm the health of animals. However, in order to maintain the biosafety of valuable and expensive horse colonies, it is important to implement a more accurate and intelligent disease control and eradication program. This enhances the laboratory diagnostic capabilities to better understand the cause of the disease and to use and optimize the diagnostic methods of glanders in the country. Therefore, efforts to further improve and optimize ELISA and Western blotting should be continue.
Type of Study: Research |
Subject:
دامپزشکی
Received: 2020/11/18 | Revised: 2022/07/17 | Accepted: 2021/06/26 | Published: 2022/03/30
Received: 2020/11/18 | Revised: 2022/07/17 | Accepted: 2021/06/26 | Published: 2022/03/30
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