Association of Exon 4 Region of Gpr54 Gene Polymorphisms with Litter Size Trait in Iranian Sanjabi and Ghezel Sheep Breeds by PCR –SSCP

ghasemi, marya; hashemi, ali; mokhber, mehdi; salehi, ronak

doi:10.52547/rap.11.29.116

Volume 11, Issue 29 (10-2020) Res Anim Prod 2020, 11(29): 116-123 | Back to browse issues page

‎ 10.52547/rap.11.29.116

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ghasemi M, hashemi A, mokhber M, salehi R. (2020). Association of Exon 4 Region of Gpr54 Gene Polymorphisms with Litter Size Trait in Iranian Sanjabi and Ghezel Sheep Breeds by PCR –SSCP. Res Anim Prod. 11(29), 116-123. doi:10.52547/rap.11.29.116
URL: http://rap.sanru.ac.ir/article-1-1043-en.html

Association of Exon 4 Region of Gpr54 Gene Polymorphisms with Litter Size Trait in Iranian Sanjabi and Ghezel Sheep Breeds by PCR –SSCP

Marya Ghasemi¹, Ali Hashemi ^*¹, Mehdi Mokhber¹, Ronak Salehi²

1- Department of Animal Science, Faculty of Agriculture, Urmia University
2- Tabriz University

Abstract: (2710 Views)

G-protein-coupled receptor (GPR54) is one of the known gene affecting fecundity. This gene affects the ovulation rate and litter size performance. A total of 160 animals including Sanjabi (n=100) and Ghezel (n=60) were used to identify polymorphisms of GPR54 gene and their influence on litter size. Genomic DNA was extracted by commercial DNA kit. The genotypic patterns were detected using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The genotypic patterns frequencies for three detected patterns in Sanjabi sheep were 54.93, 17.59 and 17.59 percent, and for two detected patterns of Ghezel were 41.86, 58.14 percent, respectively. Significant association (P<0.05) was identified between detected genotypes with litter size in Sanjabi, but no significant association (P>0.05) was found between detected polymorphisms with litter size in Ghezel sheep. Although the results showed that the detected polymorphisms in this gene can be used as a marker for twinning in Sanjabi sheep but, it may be necessary to carry out further studies with larger sample sizes to find an exact correlation between GPR54 gene variants and fecundity trait.

Keywords: Litter Size, GPR54 Gene, Sheep, PCR- SSCP

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Type of Study: Research | Subject: ژنتیک و اصلاح نژاد دام
Received: 2019/09/10 | Accepted: 2019/11/9