دوره 11، شماره 27 - ( بهار 1399 )                   جلد 11 شماره 27 صفحات 9-17 | برگشت به فهرست نسخه ها

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shahravan S, ghoorchi T. Study of Cellulase Enzymes Activity in Rumen Fluid of Fattening Slaughtered Goat Kids. rap. 2020; 11 (27) :9-17
URL: http://rap.sanru.ac.ir/article-1-964-fa.html
شه روان صفورا، قورچی تقی. بررسی فعالیت آنزیم‌های سلولاز در مایع شکمبه بز‌غاله‌های پرواری کشتار شده. پژوهشهاي توليدات دامي. 1399; 11 (27) :9-17

URL: http://rap.sanru.ac.ir/article-1-964-fa.html


دانشکده علوم دامی دانشگاه کشاورزی و منابع طبیعی گرگان
چکیده:   (207 مشاهده)
     هدف از این پژوهش بررسی فعالیت آنزیم‌های شکمبه‌ای کربوکسی متیل سلولاز، میکروکریستالین سلولاز (نانومول در دقیقه) از بخش‌های مختلف شیرابه شکمبه شامل بخش وابسته به ذرات، خارج سلولی و درونسلولی بود. برای این منظور از مایع شکمبه 15 رأس بز‌غاله‌های پرواری نجدی کشتار شده در سن ۶ ماهگی با میانگین وزن ۲۸ کیلوگرم در 3 تیمار و 5 تکرار نمونه‌برداری شد.  تیمار‌های آزمایشی شامل سطوح صفر، 10 و 20 درصد تفاله زیتون جایگزین دانه جو بود. نتایج نشان­ داد که بیشترین میزان فعالیت آنزیمی برای هر دو آنزیم در بخش وابسته به ذرات مربوط به بز‌غاله‌های تغذیه‌شده با جیره 20 درصد تفاله زیتون بود (0۵/0>P). همچنین بیشترین فعالیت کل آنزیم‌های میکروکریستالین سلولاز و کربوکسی متیل سلولاز در شکمبه بز‌غاله‌های تغذیه ‌شده با جیره 20 درصد تفاله زیتون (نانومول در دقیقه) نسبت به تیمار‌های دیگر مشاهده شد (0001/0>P). میزان فعالیت کربوکسی متیل سلولاز در بخش جامد، خارج سلولی، درون ‌سلولی و کل شیرابه شکمبه نسبت به فعالیت آنزیم میکروکریستالین سلولاز در بخش­های مربوطه بیشتر بود. pH مایع شکمبه بز‌غاله‌های با افزایش سطوح تفاله زیتون در جیره کاهش معنی­ داری یافت (0۰۰۱/0>P). کمترین pH در بز‌غاله‌های تغذیه ‌شده با 20 درصد تفاله زیتون نسبت به سایر تیمار‌ها دیده شد. درمجموع، نتایج آزمایش نشان­ داد با افزایش سطح تفاله زیتون در جیره، فعالیت آنزیم­های سلولولیتیک در هر سه بخش محتویات شکمبه افزایش یافت. فعالیت آنزیم‌ها در بخش وابسته به ذرات شیرابه شکمبه در تمام تیمارها بیشترین و بخش خارج سلولی کمترین بود (05/0P<).
متن کامل [PDF 502 kb]   (47 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تغذیه نشخوارکنندگان
دریافت: 1397/8/5 | ویرایش نهایی: 1399/2/23 | پذیرش: 1398/8/21 | انتشار: 1399/2/23

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