دوره 8، شماره 17 - ( پاییز 1396 )
جلد 8 شماره 17 صفحات 200-194 |
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Akbari H, Dashab G, Vafaye Valleh M. (2018). Exploration of MEG9 Gene Polymorphism and Its Association with Birth Weight in Sistani Cattle. Res Anim Prod. 8(17), 194-200. doi:10.29252/rap.8.17.194
URL: http://rap.sanru.ac.ir/article-1-853-fa.html
URL: http://rap.sanru.ac.ir/article-1-853-fa.html
اکبری حسین، داشاب غلامرضا، وفای واله مهدی. بررسی چندشکلی ژن MEG9 و ارتباط آن با وزن تولد در گاوهای سیستانی پژوهشهاي توليدات دامي 1396; 8 (17) :200-194 10.29252/rap.8.17.194
چکیده: (4119 مشاهده)
به منظور بررسی چندشکلی ژن MEG9 و ارتباط آن با وزن تولد در گاوهای سیستانی، از تعداد 130 رأس گوساله (نر و ماده) مرکز بهگزینی گاو سیستانی به طور تصادفی خونگیری به عمل آمد. استخراج DNA از خون کامل به روش فنل-کلروفرم انجام شد. واکنش زنجیرهای پلیمراز (PCR) جهت تکثیر قطعه 210 جفت بازی واقع در اگزون 3 ژن مذکور با استفاده از آغازگرهای اختصاصی انجام شد. شناسایی جهشها با روش SSCP با الکتروفورز محصولات پیسیآر بر روی ژل پلیآکریلآمید 8 درصد و رنگآمیزی نیترات نقره انجام گرفت. به منظور بررسی ارتباط الگوهای شناسایی شده در جایگاه ژنی MEG9 با وزن تولد در گوسالههای گاو سیستانی از مدلهای ثابت خطی و مقایسه میانگینها با روش توکی-کرامر انجام گرفت. نتایج مطالعه در جایگاه ژنی MEG9 سه الگوی باندی (ژنوتیپ) q1، q2 و q3 را به ترتیب با فراوانیهای 37، 34 و 29 درصد نشان داد. مطالعه همبستگی الگوهای مشاهده شده در جایگاه ژنی MEG9با صفت وزن تولد معنیدار بود (05/0P<). در نهایت، نتایج این مطالعه نشان داد که ژن MEG9 میتواند یک نشانگر مولکولی مناسب جهت انتخاب وزن تولد در گاوهای سیستانی باشد.
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