Volume 8, Issue 16 (11-2017)
rap 2017, 8(16): 177-182 |
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(2017). Association of Genetic Variation in Exon 1 and 3 of FSHB Gene with Litter Size in Baluchi Sheep. rap. 8(16), 177-182. doi:10.29252/rap.8.16.177
URL: http://rap.sanru.ac.ir/article-1-820-en.html
URL: http://rap.sanru.ac.ir/article-1-820-en.html
Abstract: (3047 Views)
This research was conducted to find association of genetic variation in exon 1 and 3 of the follicle stimulating hormone beta (FSHB) subunit gene and litter size in Baluchi sheep. DNA was extracted using modified salting out method and polymerase chain reaction was used to amplify a fragment of 220 bp of exon 1 and a fragment of 427 bp of exon 3 along with a part of intron 2. Two methods of PCR-SSCP and DNA sequencing were used for identification the different genotypes, While no polymorphism was found for exon 3, the present study identified 3 novel nucleotide polymorphisms in exon 1 of ovine FSHB gene, resulted 7 banding patterns which significantly influenced total and average number of labs across all parties. Results also showed significant association (p<0.05) of polymorphism in exon 1 with litter size for parity 2 in a way that pattern E had the most phenotype (1.88) compared with pattern A as reference while the less phenotype was detected for pattern B. These results showed the potential usefulness of this gene in marker-assisted selection for sheep breeding.
Type of Study: Research |
Subject:
Special
Received: 2017/10/30 | Accepted: 2017/10/30 | Published: 2017/10/30
Received: 2017/10/30 | Accepted: 2017/10/30 | Published: 2017/10/30
References
1. An, X.P., D. Han, J.X. Hou, G. Li, Y.N. Wang, L. Li, G.Q. Zhu, J.G. Wang, Y.X. Song and B.Y. Cao. 2010. Polymorphism of exon 2 of FSHβ gene and its relationship with reproduction performance in two goat breeds. Agricultural Sciences in China, 9: 880-886. [DOI:10.1016/S1671-2927(09)60167-X]
2. Artimo, P., M.J. onnalagedda, K. Arnold, D. Baratin, G. Csardi and E. DeCastro. 2012. ExPASy: SIB bioinformatics resource portal. Nucleic Acids Research, 40: 597-603. [DOI:10.1093/nar/gks400]
3. Bennett, G.L. and K.A. Leymaster. 1989. Integration of ovulation rate, potential embryonic viability and uterine capacity into a model of litter size in swine. Journal of Animal Science, 67: 1230-1241. [DOI:10.2527/jas1989.6751230x]
4. Byun, S.O., Q. Fang, H. Zhou and J.G.H. Hickford. 2009. An effective method for silver-staining DNA in large numbers of polyacrylamide gels. Analytical Biochemistry, 385: 174-175. [DOI:10.1016/j.ab.2008.10.024]
5. Fan, Q.R. and W.A. Hendrickson. 2005. Structure of human follicle-stimulating hormone in complex with its receptor. Nature, 433: 269-277. [DOI:10.1038/nature03206]
6. Farhadi, A., V. Genualdo, A. Perucatti, S.H. Hafezian, G. Rahimi-Mianji, L. De Lorenzi, P. Parma, L. Iannuzzi and A. Iannuzzi. 2013. Comparative FISH mapping of BMPR1B, BMP15 and GDF9 fecundity genes on cattle, river buffalo, sheep and goat chromosomes. Journal of Genetics, 92: 595-597. [DOI:10.1007/s12041-013-0301-8]
7. Fox, K.M., J.A. Dias and P. Van Roey. 2001. Threedimensional structure of human follicle-stimulating hormone. MolecularEndocrinoligy, 15: 378-389. [DOI:10.1210/mend.15.3.0603]
8. Galloway, S.M., K.P. McNatty, L.M. Cambridge, M.P.E. Laitinen, J.L. Juengel, S. Jokiranta, R.J. McLaren, K. Luiro, K.G. Dodds, G.W. Montgomery, A.E. Beattie, G.H. Davis and O. Ritvos. 2000. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nature Genetics, 25:279-283. [DOI:10.1038/77033]
9. Gholizadeh, M., G. Rahimi-mianji, A. Nejati-javaremi, D.J. De Koning and E. Jonas. 2015. Genomewide association study to detect QTL for twinning rate in Baluchi sheep. Journal of Genetics, 93: 489-493. [DOI:10.1007/s12041-014-0372-1]
10. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95-98.
11. Hediger, R., S.E. Johnson and DJ. Hetzel. 1991. Localization of the beta-subunit of follicle stimulating hormone in cattle and sheep by in situ hybridization. Animal Genetics, 22: 237-44. [DOI:10.1111/j.1365-2052.1991.tb00673.x]
12. Hanrahan, J.P., S.M. Gregan, P. Mulsant, M. Mullen, G.H. Davis, R. Powell and S. Galloway. 2004. Mutations in the genes for oocyte derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovisaries). Biology Reproduction, 70: 900-909. [DOI:10.1095/biolreprod.103.023093]
13. Kimchi-Sarfaty, C., J.M. Oh, I.W. Kim, Z.E. Sauna, A.M. Calcagno, S.V. Ambudkar and M.M. Gottesman. 2007. A 'silent' polymorphism in the MDR1 genechanges substrate specificity. Science, 315: 525-528. [DOI:10.1126/science.1135308]
14. Kumar, T.R., Y. Wang, N. Lu and M.M. Matzuk. 1997. Follicle stimulating hormone is required for ovarian folliclematuration but not male fertility. Nature Genetics, 15: 201-204. [DOI:10.1038/ng0297-201]
15. McLahlan, R.I., N.G. Wreford, D.M. Robertson and D.M. de Kretser. 1995. Hormonal Control of Spermatogenesis. Trends in Endocrinology and Metabolism, 6: 95-101. [DOI:10.1016/1043-2760(94)00215-P]
16. Miller, S.A., D.D. Dykes and H.F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16:1215. [DOI:10.1093/nar/16.3.1215]
17. Nazifi, N., G. Rahimi-Mianji and Z. Ansari-Pirsaraei. 2011. Association of polymorphism in FSHB gene with litter size in Iran Black, Arman and Baluchi sheep. The 12th Iranian Genetics Congress. Tehran-Iran, (In Persian).
18. Ohta, T., H. Miyake, C. Miura, H. Kamei, K. Aid and T. Miura. 2007. Follicle-stimulating hormone induces spermatogenesis mediated by androgen production in Japanese eel, Anguilla japonica. Biology Reproduction, 77: 970-977. [DOI:10.1095/biolreprod.107.062299]
19. SAS. 2009. SAS Statistical Analysis Systems 2000. User's Guide. SAS Institute Incorporation, Cary, NC.
20. Souza, C.J.C., B.K. MacDougall, A. Campbell, S. McNeilly and D.T. Baird. 2001. The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B (BMPR1B) gene. Journal of Endocrinology, 169: 1-6. [DOI:10.1677/joe.0.169r001]
21. Waldron, D.F. and D.L. Thomas. 1992. Increased litter size in Rambouillet sheep: I. Estimation of genetic parameters. Journal of Animal Science, 70: 3333-3344. [DOI:10.2527/1992.70113333x]
22. Zhang, C.Y., C.J. Wu, W.B. Zeng, K.K. Huang, X. Li, J.H. Feng, D. Wang, G.H. Hua, D.Q. Xu, Q.Y. Wen and L.G. Yang. 2011. Polymorphism in exon 3 of follicle stimulating hormone beta (FSHB) subunit gene and its association with litter traits and superovulation in the goat. Small Ruminant Research, 96: 53-59. [DOI:10.1016/j.smallrumres.2010.11.002]
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