دوره 8، شماره 16 - ( تابستان 1396 )
جلد 8 شماره 16 صفحات 165-158 |
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(2017). Detection of Polymorphism in Exon 2 of GDF9 gene in Pure and Crossbred of Pakistani Sheep. rap. 8(16), 158-165. doi:10.29252/rap.8.16.158
URL: http://rap.sanru.ac.ir/article-1-817-fa.html
URL: http://rap.sanru.ac.ir/article-1-817-fa.html
خدابخش زاده رسول، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، مرادی شهربابک حسین. شناسایی چندشکلی در اگزون دوم ژن GDF9در گوسفندان خالص و آمیخته پاکستانی پژوهشهاي توليدات دامي 1396; 8 (16) :165-158 10.29252/rap.8.16.158
چکیده: (4038 مشاهده)
ژن GDF9 از مهم ترین ژن های موثر بر چندقلوزایی گوسفندان می باشد. لذا، هدف از انجام این تحقیق شناسایی چندشکلی های تکنوکلئوتیدی موجود در اگزون دوم ژن GDF9در گوسفندان خالص و آمیخته پاکستانی به روش PCR-SSCP بود. به این منظور، نمونه های خون از 30 رأس گوسفند پاکستانی، 17 رأس گوسفند آمیخته پاکستانی و لری بختیاری و 7 رأس گوسفند آمیخته پاکستانی و کرمانی تهیه و استخراج DNA به روش نمکی بهینه انجام شد. با استفاده از یک جفت پرایمر اختصاصی یک قطعه 634 جفت بازی با کمک واکنش زنجیرهای پلیمراز تکثیر شد. پس از تعیین چند شکلی فضایی تک رشته ای محصولات PCR، الگوهای باندی مربوط به ژن GDF9 روی ژل پلی اکریلآمید 8 درصد رنگ آمیزی شده با نیترات نقره مشاهده شد. در مجموع در سه جمعیت مورد مطالعه، چهار الگوی باندی 1، 2، 3 و 4 با فراوانی های 093/0، 129/0، 371/0 و 407/0 بدست آمد. نتایج توالییابی منجر به شناسایی سه جهش در موقعیتهای نوکلئوتید 443، 477 و 721 ژن GDF9 در جمعیتهای مورد مطالعه گردید.
نوع مطالعه: پژوهشي |
موضوع مقاله:
تخصصي
دریافت: 1396/8/8 | ویرایش نهایی: 1396/8/9 | پذیرش: 1396/8/8 | انتشار: 1396/8/8
دریافت: 1396/8/8 | ویرایش نهایی: 1396/8/9 | پذیرش: 1396/8/8 | انتشار: 1396/8/8
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