Volume 8, Issue 16 (11-2017)
Res Anim Prod 2017, 8(16): 152-157 |
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(2017). Investigation of Relationship between DRB2 Gene Polymorphism and egg number of Marshallagia Marshalli Parasites in Gastrointestinal of Ghezel Sheep Breed
. Res Anim Prod. 8(16), 152-157. doi:10.29252/rap.8.16.152
URL: http://rap.sanru.ac.ir/article-1-816-en.html
URL: http://rap.sanru.ac.ir/article-1-816-en.html
Abstract: (3917 Views)
Parasites and intestinal worms are one of the main causes of the economic decline in the sheep industry. This research carried out for consideration of microsatellite polymorphism in the intron 5 of DRB2 gene and its association with the egg numbers of Marshallagia Marshalli parasite in Ghezel sheep breed, Blood and fecal samples were obtained from 80 male lambs at the age of 4-6 months. Fecal samples were collected from the lambs' rectum and the number of fecal eggs was calculated by the Clayton Lane technique. The DNA was extracted using Chloroform- Isoamyl Alcohol and Microsatellite regions of intron 5 of the DRB2 gene were amplified. PCR products were electrophoresed on 3% Agarose gel. Allele sizes were determined by 25 bp ladder and UVidoc software. The PROC MIXED was considered for evaluation of genotypes effects on the traits. Statistical analysis showed there was significant correlation between the numbers of Marshallagia Marshalli parasite eggs and the polymorphism of DRB2 gene. Therefore, lambs that had the genotypes of 288-278 and 288-288 in intron 5 of DRB2 gene had significantly (P<0.01) lower number of eggs compared with the others. In conclusion, the polymorphism at this locus can be utilized as a useful tool in the selection programs based on markers.
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