Volume 8, Issue 16 (11-2017)                   rap 2017, 8(16): 145-151 | Back to browse issues page

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DNA Extraction from Hair Roots using Modified Salting out Method. rap. 2017; 8 (16) :145-151
URL: http://rap.sanru.ac.ir/article-1-815-en.html
Abstract:   (1263 Views)
The reliability and performance of the molecular diagnostic assays such as polymerase chain reaction (PCR), enzyme digestion and other applications are influence by quantity and quality of the extracted DNA strongly. Furthermore, to choose suitable and optimal tissue for genomic experiments nevertheless consider quantity and quality of the extracted DNA, we need to consider the comfort of sample collecting, transporting costs, storing costs, safety of sampling and pre-processing. I this study an efficient salting out-modified procedure for extracting DNA from hair roots, introduced. This procedure yield high quantity of DNA with adequate quality. Four hundred Hair roots and blood samples were obtained from buffalo units experiment. The yield of extracted DNA and the purity of the DNA samples were evaluated by absorbance (A2260/A280) ratio. 1% agar gel electrophoresis and PCR amplification. Mean DNA yields and the 260/280 nm absorbance ratio for buffalo hair roots and blood were 27.67 μg and 1.85 and 17.86 μg and 1.83, respectively. Comparing this results with previous studies demonstrate that our modified procedure is cost-effectiveness, comfortable, safe, efficient and recommendable procedure to carry out in biotechnology laboratories.
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Type of Study: Research | Subject: Special
Received: 2017/10/30 | Revised: 2017/11/11 | Accepted: 2017/10/30 | Published: 2017/10/30

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