Volume 13, Issue 36 (7-2022)                   rap 2022, 13(36): 104-113 | Back to browse issues page


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Najafi A, Daghigh Kia H, Mehdipour M, Mohammadi H. (2022). Improving the Quality of Rooster Sperm during Storage at 4 °C by adding Quercetin in the form of nano-Liposomes and NLC in a Diluting Medium. rap. 13(36), 104-113. doi:10.52547/rap.13.36.104
URL: http://rap.sanru.ac.ir/article-1-1241-en.html
Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Iran
Abstract:   (1473 Views)

TExtended Abstract
Introduction and Objective: The low fertility rate of frozen sperm of poultry compared to other species is a serious challenge for artificial insemination in commercial flocks. This challenge may be related to some physiological characteristics of poultry sperm. On the other hand, osmotic, chemical and mechanical stresses during cooling processes are the most important reasons that cause structural, biochemical and functional changes in sperm, which results in a series of cascade phenomena that will lead to a decrease in sperm fertility. Quercetin is a bioactive compound with a plant origin, which has a polyphenolic chemical structure and has antioxidant properties. This composition, as an important flavonoid and a strong antioxidant, eliminates free radicals and reduces oxidative stress in chicken spermatogonia. The purpose of this study is to use quercetin in nano form in the diluent of rooster sperm during storage at 4 degrees.
Materials and Methods: Immediately after collecting semen from roosters, initial evaluations were done. Semen collected from all 15 roosters were mixed together and added to the dilution medium in order to eliminate individual effects. Different concentrations of quercetin (10, 15 and 20 μM) in three forms (unprotected and inside liposomal and NLC structures) added to base diluent composition. Then the diluted semen samples were gradually cooled at 4°C for 3 hours. Then, at time zero (immediately after the samples reach the temperature of 4°C), 24 and 48, the motility parameters, membrane integrity, lipid peroxidation, the percentage of abnormal sperms and the percentage of viability of the samples were evaluated.
Results: The results indicated that the experimental treatments did not have a significant effect on the evaluated parameters at zero time. During 24 hours of storage at 4 degrees, 15 μM of quercetin loaded in NLC significantly improved the parameters of sperm total motility, progressive motility, viability and integrity of sperm membrane compared to the control group. During 48 hours of storage at 4 degrees, the treatment of 15 μM quercetin loaded in NLC caused a significant improvement in the parameters of sperm total motility, progressive motility, viability and integrity of sperm membrane compared to the control group. Also, the results showed that the experimental treatments did not have a significant effect on the abnormality percentage of rooster sperm during storage at 4 degrees in three times of 0, 24 and 48 hours. During 24 and 48 hours of storage at 4 degrees, the treatment of 15 μM quercetin loaded in NLC caused a significant decrease in sperm MDA compared to the control group.
Conclusion: The results of this experiment show that the treatment of 15 μM quercetin loaded in NLC improves many evaluated parameters and therefore it is recommended for use in rooster sperm diluent.

Keywords: Antioxidant, Nano, Sperm, Rooster
Full-Text [PDF 1358 kb]   (430 Downloads)    
Type of Study: Research | Subject: فیزیولوژی
Received: 2021/10/3 | Revised: 2022/10/3 | Accepted: 2021/11/14 | Published: 2022/10/3

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