Volume 8, Issue 18 (3-2018)
rap 2018, 8(18): 147-154 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Raufian P, Shodja Ghyas J, Jafari R, Moghaddam G, Javanmard A. (2018). Identification of Genetic Variation in two Candidate Genes of TLR2 and TNFα and its Association with Mastitis in Holstain Cattle. rap. 8(18), 147-154. doi:10.29252/rap.8.18.147
URL: http://rap.sanru.ac.ir/article-1-906-en.html
URL: http://rap.sanru.ac.ir/article-1-906-en.html
Abstract: (4424 Views)
Mastitis is one of the most common diseases in dairy cattle, which has imposed therapy heavy costs to farmers through significant reduction in total milk production. Identification of nucleotide polymorphism of two candidate immunity response genes and its effects on SCS as a mastitis incidence indicator was carried out in this research. The genes were exons 1 and 2 of TLR2 and 3 and 4 of TNFα. For this purpose, a hundred Holstein lactating cows were selected based on their breeding values for milk production, fat and somatic cell scores. Then cows based on their residual values for somatic cell scores were divided into two resistant and susceptible groups and 5 ml blood samples were collected from each animal. Selective genotyping of two target groups of susceptible and resistant animals to mastitis was done using PCR-RFLP approach. The association between genetic polymorphisms with corrected records of somatic cell scores (residual values for somatic cell score) were analyzed using the Generalized Linear Model (GLM) procedure of SAS statistical package (version 9.2) followed by means comparison with Tukey-Kramer test. There was a significant direct relationship between TLR2 and TNFα gene polymorphisms and somatic cell score residual values (P<0.001). Furthermore, For TNFα gene the most genotypic frequencies in resistant and susceptible groups were related to genotypes AA and BB, respectively. Also, KK genotype frequency within TLR2 candidate gene showed a higher frequency within susceptible group versus the resistant group. As a conclusion, the obtained results may be used to monitor and design breeding strategy to prevent mastitis.
Type of Study: Research |
Subject:
Special
Received: 2018/02/28 | Revised: 2018/03/3 | Accepted: 2018/02/28 | Published: 2018/02/28
Received: 2018/02/28 | Revised: 2018/03/3 | Accepted: 2018/02/28 | Published: 2018/02/28
References
1. A-Juan, X.U., L.I. U. Xiao-Lin, G.U.O. Jia-Zhong and X.I.A. Zhi. 2009. Polymorphism of bovine TNF- gene and its association with mastitis in Chinese Holstein cows. Hereditas (Beijing), 32(9): 929-934.
2. Atashpaz, A., A. Barzegari and R. Azarbaijani. 2008. General and DNA extraction kit. Iranian patent office. No. 48024.
3. Anoosheh, S., P. Farnia and M. Kargar. 2010. Association between TNF-Alpha (-857) gene polymorphism and susceptibility to tuberculosis. Iran Red Crescent Med J., 13(4): 243-248.
4. Beecher, C., D. Mairead, C. Stuart, B. Donagh, A. Magee David, V. McCarthy Tommie and L. Giblin. 2010. Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genet, 11(99): 1-9. [DOI:10.1186/1471-2156-11-99]
5. Carvajal, A.M., P. Huircan and A. Lepori. 2013. Single nucleotide polymorphisms in immunity-related genes and their association with mastitis in Chilean dairy cattle. Genetics and Molecular Research, 12(3): 2702-2711. [DOI:10.4238/2013.July.30.8]
6. Dadpasand, M., M.J. Zamiri, H. Atashi and A. Akhlaghi. 2012. Genetic relationship of conformation traits with average somatic cell score at 150 and 305 days in milk in Holstein cows of Iran. Journal Dairy Science, 95: 7340-7345. [DOI:10.3168/jds.2011-5002]
7. Bhatt, D., P.S. Khade, S.B. Tarate, A.K. Tripathi, D.S. Nauriyal, D.N. Rank, A.P. Kunjadia and C.G. Joshi. 2012. Cytokine expression pattern in milk somatic cells of subclinical mastitis-affected cattle analyzed by real time PCR. Korean Journal Veterinary, 52(4): 231-238.
8. Fonseca, G.R., D.S. Antunes, C. Paiva, S. Lange, F. Guimarães and M.F. Martins. 2011. Differential expression of genes during mastitis in Holstein-Zebu crossbreeds dairy cows. Genetic Molecular Research, 10(3): 1295-1303. [DOI:10.4238/vol10-3gmr1096]
9. Fonseca, V., C. Silva, M. Lange, C. Guimaraes, A.K. Weller, P. Sousa, S. Lopes and S. Guimaraes. 2009. Expression profile of genes associated with mastitis in dairy cattle. Genetics and molecular biology, 32(4): 776-781. [DOI:10.1590/S1415-47572009005000074]
10. Ghazanfari Kherabadi, M.R. 2011. Polymorphisms TLR4 gen and association with somatic cell score in Holestein and Brown Swiss in khorasan. Master's thesis, University Birjand, pp: 5-7 (In Persian).
11. Hand, K.J., A. Godkin and D.F. Kelton. 2012. Milk production and somatic cell counts: A cow-level analysis. Journal of Dairy Science, 95: 1358-1362. [DOI:10.3168/jds.2011-4927]
12. Hagnestam-Nielsen, C., Emanuelson, B. Berglund and E. Strandberg. 2009. Relationship between somatic cell count and milk yield in different stages of lactation. Journal Dairy Science, 92: 3124-3133. [DOI:10.3168/jds.2008-1719]
13. Hillerton, J.E. 1999. Balancing mastitis and quality. The Proceeding of the British Mastitis Conference, 31-36.
14. Jamali, N., A. Sadeghi Sefid Mazgi and M.M. Moeini. 2013. Evaluation of milk somatic cell count in dairy farms of industrial and traditional in Tehran. Livestock production, 14(1): 29-21 (In Persian).
15. Katsafadou, A.I. and G.C. Fthenakis. 2013. Breeding for mastitis resistance in sheep and goats. Journal Veterinary Science Technology, 4(4): 71 pp.
16. Kawasaki, Y., A. Yuka, F. Magata, A. Miyamoto, C. Kawashima, T. Hojo, K. Okuda, S. Koumei and T. Shimizu. 2014. The Effect of Single Nucleotide Polymorphisms in the Tumor necrosis factor on mastitis in dairy cattle. Journal of Dairy Science, 96: 1359-1363.
17. Lievaart. J., W. Kremer and H. Barkema. 2007. Short Communication: Comparison of Bulk Milk, Yield-Corrected, and Average Somatic Cell Counts as Parameters to Summarize the Subclinical Mastitis Situation in a Dairy Herd. Journal of Dairy Science, 90: 4145-4148. [DOI:10.3168/jds.2006-871]
18. Martins, A.M., A.M. Silvestre, M.F. Petim-Batista and J.A. Colaco. 2011. Somatic cell score genetic parameter estimates of dairy cattle in Portugal using fractional polynomials Journal of Animal Science, 89: 1281-1285. [DOI:10.2527/jas.2010-3211]
19. Miller, R.H., H.D. Norman, G.R. Wiggans and J.R. Wright. 2004. Relationship of test-day somatic cell score with test-day and lactation milk yields. Journal of Dairy Science, 87: 2299-2306. [DOI:10.3168/jds.S0022-0302(04)70051-X]
20. Naghshineh, S. 2011. Evaluation of inbreeding and its effect on milk production and subclinical mastitis in dairy cows. Master's thesis. University of Tabriz, pp: 4-5 (In Persian).
21. Ranjan, S., B. Bhushan, M. Panigrahi, A. Kumar, R. Deb, P. Kumar and D. Sharma. 2015. Association and Expression Analysis of Single Nucleotide Polymorphisms of Partial Tumor Necrosis Factor Alpha Gene with Mastitis in Crossbred Cattle. Animal Biotechnology, 26: 98-104, 2015. [DOI:10.1080/10495398.2014.929582]
22. Samore, A. and A. Groen. 2006. Proposal of an udder health genetic index for the Italian Holstein Friesian based on first lactation data. Italian Journal Animal Science, 5: 359-370. [DOI:10.4081/ijas.2006.359]
23. Sargolzaei, M. 2006. CFC, A software package for pedigree analysis and monitoring genetic diversity. Course of Environmental Management Science, Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan.
24. Swiderek, W.P., M.R. Bhide, J. Gruszczynska, K. Soltis, D. Witkowska and I. Mkula. 2006. Toll like receptor gene polymorphism and its relationship with somatic cell concentration and natural bacterial infections of the mammary gland in sheep. Folial microbial, 51(6): 647-652. [DOI:10.1007/BF02931633]
25. Wojdak-Maksymiec, K., J. Szyda and T. Strabel. 2013. Parity-dependent association between TNF-α and LTF gene polymorphisms and clinical mastitis in dairy cattle. BMC Veterinary Research, 9(114):1-8. [DOI:10.1186/1746-6148-9-114]
26. Xu, A.J., X.L. Liu, J.Z. Guo and Xia. Z. 2010. Polymorphism of bovine TNF-a gene and its association with mastitis in Chinese Holstein cows. Yi Chuan, 32(9): 929-34.
27. Yeh, F.C., R.C. Yang, T.B.J. Boyle, Z.H. Ye and J.X. Mao. 1997. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
28. Zhang, L.P., Q.F. Gan, T.H. Ma, H.D. Li, X.P. Wang, J.Y. Li, X. Gao, J.B. Chen, H.Y. Ren and S.Z. Xu. 2009. Toll-like receptor 2 gene polymorphism and its relationship with SCC in dairy cattle. Animal Biotechnology, 20: 87-95. [DOI:10.1080/10495390902873096]
29. Zamiri, M.J. 2006. Trining of dairy cattle. 6nd edn, Shiraz University Press, pp: 75-85 (In Persian).
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
