Volume 8, Issue 18 (3-2018)                   rap 2018, 8(18): 107-112 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abdi Benemar H, Seifdavati J, Fathi Achachlouei B, kachuee R. Comparison of Hach Method with Kjeldahl Method for Determining of Crude Protein Contents of Some Animal Feeds. rap 2018; 8 (18) :107-112
URL: http://rap.sanru.ac.ir/article-1-901-en.html
Abstract:   (3312 Views)
The aim of present study was to evaluation of Hach method for determining of crude protein contents of some animal feeds. Twenty one samples of animal feeds and digesta, including a variety of forages, concentrate feeds and mixtures and digesta ruminal samples and 0.025, 0.05, 0.075, 0.1, 0.175 and 0.2 mg ammonium chloride were chosen and their nitrogen and protein contents were measured using Kjeldahl, as the reference method and the Hach Methods and compared with each other. There was no significant difference between the methods in terms of crude protein content estimation of different feed samples and different amounts of ammonium chloride. The regression between the crude protein contents measured by the two methods was linear and showed a significant correlation (r=0.99). Modifing the digestion method of the original Hach method accelerate the method and it makes possible to analysis more samples without any negative effects on the obtained results. According with the reslt of this experiment the protein content of some analyzing samples in animal nutrition laboratories can be accurately measured through the Hach method.
Full-Text [PDF 970 kb]   (5757 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/02/28 | Revised: 2018/03/3 | Accepted: 2018/02/28 | Published: 2018/02/28

References
1. AOAC. 2000. Official Methods of Analysis. 17th ed. Arlington (VA): Association of official analytical chemists.
2. Barbarino, E. and S.O. Lourenço. 2009. Comparison of CHN analysis and Hach acid digestion to quantify total nitrogen in marine organisms. Limnology and oceanography: methods, 7: 751-760. [DOI:10.4319/lom.2009.7.751]
3. Bradstreet, R.B. 2013. The Kjeldahl Method for Organic Nitrogen. Elsevier, pp: 39-88.
4. Figenschou, D.L., J.P. Marais and M. De Figueiredo. 2000. A comparison of three methods of nitrogen analysis for feedstuffs. South African Journal of Animal Science: Supplement, 1: 23-23. [DOI:10.4314/sajas.v30i4.3892]
5. Florence, E. and D.F. Milner. 1979. Routine determination of nitrogen by Kjeldahl digestion without use of catalyst. Analyst, 104: 378-381. [DOI:10.1039/an9790400378]
6. Fukumoto, H.E. and G.W. Chang. 1982. Manual salicylate-hypochlorite procedure for determination of ammonia in Kjeldahl digests [Urine and faeces analysis, mankind, urine, faeces, nitrogen content, digestion, spectrometry, methods, chemical analyses]. Journal of the Association of Official Analytical Chemists, 65: 1076-1079.
7. Hach, C.C., B.K. Bowden, A.B. Kopelove and S.T. Brayton. 1987. More powerful peroxide Kjeldahl digestion method. Journal of the Association of Official Analytical Chemists, 70: 783-787.
8. Hach, C.C., S.V. Brayton, and A.B. Kopelove. 1985. A powerful Kjeldahl nitrogen method using peroxymonosulfuric acid. Journal of Agriculter and Food Chemistry, 33: 1117-1123. [DOI:10.1021/jf00066a025]
9. Kjeldahl, J. 1883. A new method for the estimation of nitrogen in organic compounds. Zeitschrift für Analytische Chemie, 22: 366-382. [DOI:10.1007/BF01338151]
10. Miller G.L. and E.E. Miller. 1948. Determination of nitrogen in biological materials. Analytical Chemistry, 20(5): 481-488. [DOI:10.1021/ac60017a022]
11. National Research Council. 2001. Nutrient Requirement of Dairy Cattle. 6th rev. ed. National Acadmic Science. Washington. DC.
12. Osborne, R.A. and J.B. Wilkie. 1935. A study of the Kjeldahl method. III-Further comparisons of selenium with mercury and with copper catalysts. journal of Association official Agricultural Chemists, 18: 604-609.
13. Reynolds, C.K. and N.B. Kristensen. 2008. Nitrogen recycling through the gut and the nitrogen economy of ruminants: An asynchronous symbiosis. Journal of animal science, 86: 293-305. [DOI:10.2527/jas.2007-0475]
14. Rossi, A.M., M. Villarreal, M.D. Juarez and N.C. Samman. 2004. Nitrogen contents in food: A comparison between the Kjeldahl and Hach methods. The journal of the Argentine Chemical Society, 92: 99-108.
15. Sahoo, B. and T.K. Walli. 2008. Effect of feeding undegradable protein with energy on nutrient utilization, milk yield and milk composition of crossbred goats. Small Ruminant Research, 75: 36-42. [DOI:10.1016/j.smallrumres.2007.07.007]
16. Wall, L.L., G.W. Gehrke, T.E. Neuner, R.D. Cathey and P.R. Rexroad. 1975. Total protein nitrogen: evaluation and comparison of four different methods. Journal of the Association of Official Analytical Chemists. 58: 1221-1226.
17. Watkins, K.L., T.L. Veum and G.F. Krause. 1986. Total nitrogen determination of various sample types: a comparison of the Hach, Kjeltec and Kjeldahl methods. Journal of the Association of Official Analytical Chemists, 70: 410-412.

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Research On Animal Production(Scientific and Research)

Designed & Developed by : Yektaweb