1- Tabriz university
Abstract: (43 Views)
Introduction and Objective: Growth rate is one of the most important economic traits in the broiler chickens. Growth rate could be modified with the influences of both genetic and non-genetics factors. Better understanding of the domestic chicken genome may help the genetic improvement of chicken to be accelerated for both meat and egg yield traits. Ascites, a metabolic-genetic disorder in the meat-type chickens, is associated with the chicken own susceptibility as well as with the environmental triggers including rich nutrition and bad rearing conditions. In the current research, the association of polymorphisms in two ascites-related genes (i.e., PDK4 and THRSP) with the body weight and growth-related traits was investigated.
Material and Methods: The RNA-seq data was used to discovery of the SNPs within the THRSP and PDK4 genes. After detecting the variants and identifying their corresponding RFLP markers using bioinformatics analysis, two suitable RFLP markers (each on one of the mentioned genes) were selected for the wet-lab analysis. Then, the growth differences among the different genotypes were monitored. The growth patterns of 114 chickens (consisting of 44 healthy males, 11 ascitic males, 55 healthy females, and 4 ascitic females) were analyzed using the ScaI restriction enzyme.
Results: Genotyping of the two mentioned genes showed that at PDK4 locus, the frequencies of CC, TC and TT genotypes were 0.105, 0.658 and 0.237, respectively. In the THRSP locus, the frequencies of AA, AG and GG genotypes were 0.185, 0.5 and 0.315, respectively. The growth rate and body weight least square means of the chickens of the three genotypes were significantly different neither for THRSP nor for PDK4 gene (p>0.05).
Conclusion: The observed genotypes did not show any significant differences in term of growth patterns. Since the studied genes are among the important genes affecting the incidence of ascites, investigating their combined effect on the growth-related traits was the main goal of the current work study. Therefore, the lack of association between the different genotypes of the PDK4 and THRSP genes with the growth-related traits emphasizes to the fact that there is no actually direct genetic relationship between the rapid growth rate and susceptibility of chickens to ascites. However, further assessment on the other variants harboring the studied genes may be needed to elucidate the inherent, if any, genetic association of the mentioned genes with the growth related traits.
Material and Methods: The RNA-seq data was used to discovery of the SNPs within the THRSP and PDK4 genes. After detecting the variants and identifying their corresponding RFLP markers using bioinformatics analysis, two suitable RFLP markers (each on one of the mentioned genes) were selected for the wet-lab analysis. Then, the growth differences among the different genotypes were monitored. The growth patterns of 114 chickens (consisting of 44 healthy males, 11 ascitic males, 55 healthy females, and 4 ascitic females) were analyzed using the ScaI restriction enzyme.
Results: Genotyping of the two mentioned genes showed that at PDK4 locus, the frequencies of CC, TC and TT genotypes were 0.105, 0.658 and 0.237, respectively. In the THRSP locus, the frequencies of AA, AG and GG genotypes were 0.185, 0.5 and 0.315, respectively. The growth rate and body weight least square means of the chickens of the three genotypes were significantly different neither for THRSP nor for PDK4 gene (p>0.05).
Conclusion: The observed genotypes did not show any significant differences in term of growth patterns. Since the studied genes are among the important genes affecting the incidence of ascites, investigating their combined effect on the growth-related traits was the main goal of the current work study. Therefore, the lack of association between the different genotypes of the PDK4 and THRSP genes with the growth-related traits emphasizes to the fact that there is no actually direct genetic relationship between the rapid growth rate and susceptibility of chickens to ascites. However, further assessment on the other variants harboring the studied genes may be needed to elucidate the inherent, if any, genetic association of the mentioned genes with the growth related traits.
Type of Study: Research |
Subject:
ژنتیک و اصلاح نژاد طیور
Received: 2023/09/8 | Accepted: 2025/06/1
Received: 2023/09/8 | Accepted: 2025/06/1
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