Volume 14, Issue 3 (10-2023)
Res Anim Prod 2023, 14(3): 105-115 |
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Akhundpour N, Hashemi S R, Farhadi A, Arabian E. (2023). Studying the Effect of Zeolite, Organic Acid and Silver Nanoparticles Coated on Zeolite on the Expression of BAX and Bcl2 Apoptosis Genes in the Liver of Broiler Chickens. Res Anim Prod. 14(3), 105-115. doi:10.61186/rap.14.41.105
URL: http://rap.sanru.ac.ir/article-1-1320-en.html
URL: http://rap.sanru.ac.ir/article-1-1320-en.html
1- Department of Animal Physiology, Faculty of Animal Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2- Department of Animal Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
2- Department of Animal Sciences, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran
Abstract: (1701 Views)
Extended Abstract
Introduction and Objective: Silver nanoparticles are one of the new achievements in nanotechnology, which are used in medicine, veterinary medicine, agriculture, and pharmaceuticals due to their antimicrobial activity. Silver nanoparticles can react with biological macromolecules and induce apoptosis by disrupting intracellular homeostasis. Hence, the aim of this study was to the effect of zeolite, organic acid and silver nanoparticles coated on zeolite on the expression of BAX and Bcl2 apoptosis genes in broiler chickens.
Material and Methods: The number of 450 one-day- old broiler chicks (Cobb 500) were used in five treatments and six repetitions with 15 chicks per replicate in the form of a completely random design. Experimental diets were: control or basal diet (1), a basal diet supplemented by 1% zeolite (2), a basal diet supplemented by 1% zeolite coated with 0.5% nanosilver (3), a basal diet supplemented by 0.15% organic acid (4) and a basal diet supplemented by 1% zeolite coated with 0.5% nanosilver and 0.15% organic (5). The experiment was conducted with broiler chicken from 1 to 42 days old. The expression of the BCL2 and BAX apoptosis genes, liver sampling was done on the 21st and 42nd days of the breeding period.
Results: The results of this experiment showed that in broiler chicks receiving zeolite treatment (Z) on d 21 and silver nanoparticles coated on zeolite treatment (NS) on d 42 of the breeding period the expression level of Bcl2 gene in liver tissue was significantly increased (P<0.05) compared to the control treatment (C Also, the expression of the BAX gene in the liver tissue in NS and NSOA treatments on d 42 of the breeding period increased significantly compared to the control treatment (P<0.05).
Conclusion: In conclusion, the results of this experiment showed that zeolite and NS and NSOA treatments have an increasing effect on the expression of Bcl2 and BAX genes, while this effect was not observed in organic acid treatment.
Introduction and Objective: Silver nanoparticles are one of the new achievements in nanotechnology, which are used in medicine, veterinary medicine, agriculture, and pharmaceuticals due to their antimicrobial activity. Silver nanoparticles can react with biological macromolecules and induce apoptosis by disrupting intracellular homeostasis. Hence, the aim of this study was to the effect of zeolite, organic acid and silver nanoparticles coated on zeolite on the expression of BAX and Bcl2 apoptosis genes in broiler chickens.
Material and Methods: The number of 450 one-day- old broiler chicks (Cobb 500) were used in five treatments and six repetitions with 15 chicks per replicate in the form of a completely random design. Experimental diets were: control or basal diet (1), a basal diet supplemented by 1% zeolite (2), a basal diet supplemented by 1% zeolite coated with 0.5% nanosilver (3), a basal diet supplemented by 0.15% organic acid (4) and a basal diet supplemented by 1% zeolite coated with 0.5% nanosilver and 0.15% organic (5). The experiment was conducted with broiler chicken from 1 to 42 days old. The expression of the BCL2 and BAX apoptosis genes, liver sampling was done on the 21st and 42nd days of the breeding period.
Results: The results of this experiment showed that in broiler chicks receiving zeolite treatment (Z) on d 21 and silver nanoparticles coated on zeolite treatment (NS) on d 42 of the breeding period the expression level of Bcl2 gene in liver tissue was significantly increased (P<0.05) compared to the control treatment (C Also, the expression of the BAX gene in the liver tissue in NS and NSOA treatments on d 42 of the breeding period increased significantly compared to the control treatment (P<0.05).
Conclusion: In conclusion, the results of this experiment showed that zeolite and NS and NSOA treatments have an increasing effect on the expression of Bcl2 and BAX genes, while this effect was not observed in organic acid treatment.
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