دوره 7، شماره 14 - ( پاییز و زمستان 1395 )
جلد 7 شماره 14 صفحات 186-192 |
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(2016). Effect of Polymorphisms in Some Candidate Genes on Twinning in Cross Breeds of Shall and Romanov. Res Anim Prod. 7(14), 192-186. doi:10.29252/rap.7.14.192
URL: http://rap.sanru.ac.ir/article-1-709-fa.html
URL: http://rap.sanru.ac.ir/article-1-709-fa.html
خان احمدی علیرضا، رحیمی میانجی قدرت، حافظیان سید حسن، خاتمی نژاد رسول، مامی زاده نوربی بی، موسوی سید ماکان. بررسی اثر چند شکلی برخی از ژنهای کاندید بر چند قلوزایی در گوسفندان آمیخته حاصل از تلاقی نژادهای شال ایرانی و رومانوف روسی پژوهشهاي توليدات دامي 1395; 7 (14) :186-192 10.29252/rap.7.14.192
علیرضا خان احمدی*، قدرت رحیمی میانجی، سید حسن حافظیان، رسول خاتمی نژاد، نوربی بی مامی زاده، سید ماکان موسوی
چکیده: (5048 مشاهده)
این تحقیق به منظور بررسی چند شکلیآللی ژنهای BMP15 (واریانت FecxG و FecxB )، GDF9 (وایانت G8 و G1) و BMPR- IB (FecB) در آمیختههای شال و رومانف انجام شد. برای این منظور 79 نمونه خون از گله موجود در سطح شهرستان گنبد کاووس جمعآوری و استخراج DNA به روش بهینه یافته نمکی انجام شد. با استفاده از پرایمرهای اختصاصی قطعات 141 و153 جفت بازی از اگزون 2 ژن BMP15 بهترتیب برای شناسایی جهشهای FecXG و FecXB قطعات 462 و 139 جفت بازی
بهترتیب از اگزونهای 1 و 2 ژن GDF9 برای شناسایی واریانتهای G1 و G8 و یک قطعه 190 جفت بازی از ژن BMPR-IB برای شناسایی جهش FecB با استفاده از واکنش زنجیرهای پلیمراز تکثیر شدند. نتایج حاصل از هضم محصولات PCR وجود جهش درجایگاه BMP15،BMPR-IB و اگزون 2 ژن GDF9 را تاًیید نکرد. در حالی که در اگزون 1 جایگاه GDF9 چندشکلی مشاهده شد، و فراوانی ژنوتیپ هتروزایگوت در این جمعیت به مقدار 11/0 برآورد شد.
فهرست منابع
1. Alimahmoodi, M., M.T. Nassiri, G.H. Mohammadi, K.H. Mirzadeh and G. Fayazi. 2009. Polymorphisms in FecB gene in Lori-Bakhtiyari and Arabi sheep with PCR-RFLP method. First Animal Science Congress in Golestan. Gorgan. Iran.
2. Amiri, A., G.H. Rahimi Mianji and M. Vatankhah. 2008. Detection allelic polymorphisms in BMP15 and GDF9 Genes and important oocyte growth factor in Lori-Bakhtiyari sheep. 5th Iranian Biotechnology Congress. Tehran. Iran.
3. Amiri, A., G.H. Rahimi Mianji and M. Vatankhah. 2009. Non detection polymorphisms in FecB and FecXI Genes in Lori-Bakhtiyari sheep. Modern Genetics, 3: 57-63.
4. Barzegari, A., S. Atashpaz, K. Ghabili, Z. Nemati, M. Rustaein and R. Azarbaijani. 2010. Polymorphisms in GDF9 and BMP15 associated with fertility and ovulation rate in Moghani and Ghezel sheep in Iran. Reprod Domest Animal, 45: 666-669.
5. Bodin, L., E. Di Pasquale, S. Fabre, M. Bontoux, P. Monget, L. Ersani and P. Mulsant. 2006. A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep. Endocrinology, 148: 393-400. [DOI:10.1210/en.2006-0764]
6. Chu, M.X., S. Wang and L. Fang. 2004. Association between PCR-SSCP of growth differentiation factor 9 genes and high prolificacy in small tail han sheep. Anim. Biotechnology, 15: 111-120. [DOI:10.1081/LABT-200032582]
7. Davis, G.H., J.C. McEwan, P.F. Fennessy, K.G. Dodds and P.A. Farquhar. 1991. Evidence for the presence of a major gene influencing ovulation rate on the X-chromosome of sheep. Biology of Reproduction, 44: 620-624. [DOI:10.1095/biolreprod44.4.620]
8. Davis, G.H., L. Balakrishnan, I.K. Ross, T. Wilson, S.M. Galloway, B.M. Lumsden, J.P. Hanrahan, M. Mullen, X.Z. Mao, G.L. Wang, Z.S. Zhao, Y.Q. Zeng, J.J. Robinson, A.P. Mavrogenis, C. Papachristoforou, C. Peter, R. Baumung, P. Cardyn, I. Boujenane, N.E. Cockett, E. Eythorsdottir, J.J. Arranz and D.R. Notter. 2006. Investigation of the Booroola (FecB) and Inverdale (FecXI) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. Animal Reproduction Science, 92: 87-96. [DOI:10.1016/j.anireprosci.2005.06.001]
9. Davis, G.H. 2004. Fecundity genes in sheep. Journal of Animal Reproduction Science, 82: 247-253. [DOI:10.1016/j.anireprosci.2004.04.001]
10. Davis, G.H., J.C. McEwan, P.F. Fennessy, K.G. Dodds and K.P. McNatty. 1992. Infertility due to bilateral ovarian hypoplasia in sheep homozygous (FecX/FecX) for the inverdale prolificacy gene located on the X chromosome. Biology of Reproduction, 46: 636-640. [DOI:10.1095/biolreprod46.4.636]
11. Escandari, G.H. and E. Ayaran. 2011. Polymorphisms in FecB in Lori-Bakhtiyari sheep with PCR-RFLP. 4th Animal Science Congress. Tehran. Iran.
12. Farajzadeh, M. 2006. Polymorphisms in BMP15 and GDF9 genes in Zel sheep and relationship with reproduction. M.Sc. Thesis. University of Mazandaran.
13. Forotanifar, S., M.R. Nassrir, F. Eftekhari Shahrodi, A. Samei and M. Soltani. 2007. Non detection polymorphisms in FecB Gene in Ghare gol, Balochi and Iran black sheep. First Agricultural Biotechnology Congress. Kermanshah. Iran.
14. Galloway, S.M., K.P. McNatty, L.M. Cambridge, M.P.E. Laitinen, J.L. Juengel, T.S. Jokiranta, R.J. McLaren, K. Luiro, K.G. Dodds, G.W. Montgomery, A.E. Beattie, G.H. Davis and O. Ritvos. 2000. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Journal of Natural Genetics, 25: 279-283. [DOI:10.1038/77033]
15. Ghaderi, A., M.T. Nassiribeyg, K.H. Mirzadeh, J. Fayazi, G.H. Mohammadi and A. Sadatsadr. 2009. Polymorphisms in GDF9 gene in Arabian sheep with PCR-RFLP method. First Animal Science congress in Golestan. Gorgan. Iran.
16. Ghafari, M., A. Nejati Javaremi and G.H. Rahimi Miyanji. 2008. Detection Allelic Polymorphisms in GDF9 Gene and important oocyte growth factor in Shall sheep with PCR-RFLP. 5th Iranian Biotechnology Congress. Tehran. Iran.
17. Ghanbari, S., R. Osfori, M. Escandari Nasab, R. Rostamkhani and R. Saeid Mohammadi. 2008. Polymorphisms in FecB gene in Afshari sheep with Molecular method. Second Iranian Animal and Fishery Sceicne. Karaj. Iran.
18. Hanrahan J.P., S.M. Gregan, P. Mulsant, M. Mullen, G.H. Davis, R. Powell and S.M. Galloway. 2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol Reprod 70: 900-909. [DOI:10.1095/biolreprod.103.023093]
19. Hanrahan, J.P. and J.B. Owen. 1985. Variation and repeatability of ovulation rate in Cambridge ewes. Animal Production, 40: 529.
20. Jamshidi, A., M. Kasiriyan, G.H. Rahimi Miyanji and M.R. Banaeiyan. 2011. Polymorphisms in GDF9 gene in Sangsari sheep with PCR-RFLP. 11th Iranian Genetics Congress. Tehran. Iran.
21. Javanmard, A., N. Azadzadeh and A.K. Esmailizadeh. 2011. Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds. Vet Res Commun, 35: 157-167. [DOI:10.1007/s11259-011-9467-9]
22. Kasiriyan, M., G.H. Rahimi Miyanji and A. Jamshidi. 2009. Polymorphisms in genes Related to Reproduction in Sangsari sheep with PCR-RFLP method. 10th Iranian Genetics Congress.
23. Khanahmadi, A.R., R. Khatami Nejad, M. Ahani Azari, S. Zereh Daran and A. Jalil. 2011. Polymorphisms in GDF9 gene in Dalagh sheep with PCR-RFLP. 11th Iranian Genetics Congress. Tehran. Iran.
24. Khanahmadi, A.R., R. Khatami Nejad, S.H. Ghareh Veysi and A. Jalil. 2011. Polymorphisms in FecB gene in Dalagh sheep with PBR method. 4th Iranian Animal Science Congress. Tehran. Iran.
25. Khanahmadi, A.R., R. Khatami Nejad, S.H. Ghareh Veysi and M. Khodadadi Sorkhi. 2011. Polymorphisms in FecB gene in Dalagh sheep with PCR-RFLP method and relationship with reproduction. 4th Iranian Animal Science Congress. Tehran. Iran.
26. Khederzadeh, S., A. Yazdanpanah, A. Mohammadi Kaftarkari and A. Salari. 2011. Polymorphisms in FecB gene in Dalagh sheep with PBR method and relationship with reproduction. 11th Iranian Genetics Congress. Tehran. Iran.
27. Knight, P.G. and C. Glister. 2006. TFG- beta superfamily members and ovarian follicle development. Reproduction, 132: 191-206. [DOI:10.1530/rep.1.01074]
28. Laitinen M.P.E., M. Cranfield, N.P. Groome, O. Ritvos and K.P. McNatty. 2002. Growth differentiation factor 9 and bone morphogenetic protein 15 are essential for ovarian follicular development in sheep. Biol Reprod, 67: 1777-1789. [DOI:10.1095/biolreprod.102.007146]
29. Miller, S.A., D.D. Dyckes and H.F. Polesky. 1988. A simple salting-outprocedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16: 1215. [DOI:10.1093/nar/16.3.1215]
30. Mirabzadeh Ardakani, A. 2010. Detection of polymorphisms and allelic frequency MTNR1A and GDF9 genes in Ghezel sheep. M.Sc. Thesis. University of Tehran.
31. Mohammadi, G.H. 2007. Polymorphisms in FecB gene in Ghezel sheep and relationship with reproduction. PhD. Thesis. University of Oromiyeh.
32. Mohammadi, G.H., M.T. Beygi Nassiri, M. Ali Mahmodi, K.H. Mirzadeh and J. Fayazi. 2010. Detection of Polymorphisms in FecB gene in Arabian and Lori-Bakhtiyari sheep in Khozestan province with PCR-RFLP method. Iranian Journal of Veterinary, 5: 54-59.
33. Mohammadi, G.H., A. Saberivand and F. Barati. 2009. Polymorphisms in FecB gene in Macoei sheep. 10th Iranian Genetic Congress. Tehran. Iran.
34. Moradband. M., G. Rahimi and M. Gholizadeh. 2011. Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep. Asian-Aust. Journal Animal Science, 24: 1179 -1183.
35. Polly, S., B. Brahma, A. Mukherjee, P.V. Vinesh, S. Batabyal, J.S. Arora, S. Pan, A. Kumar Samanta, T. Kumar Datta and S. Lal Goswami. 2010. Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep.Tropical Animal Health and Production, 42: 985-993. [DOI:10.1007/s11250-009-9518-1]
36. Ricordeau, G., J. Thimonier, I.P. Polvey, M.A. Driancourt, M.T. Hochereau-de-Reviers and L. Tchamitchian, INRA. Research on the Romanov sheep breed in France: a review. Livestock Production Science, 24: 305-332. [DOI:10.1016/0301-6226(90)90009-U]
37. Souza, C.J.H., C. MacDougall, B.K. Campbell, A.S. McNeilly and D.T. Baird. 2001. The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR-1B) gene. Endocrinology.169: 1-6. [DOI:10.1677/joe.0.169R001]
38. Vacca, G.M., A. Dhaouadi, M. Rekik, V. Carcangiu, M. Pazzola and M.L. Dettori. 2010. Prolificacy genotype at BMPR-1B, BMP15 and GDF9 genes in North African sheep breeds. Small Ruminant Research, 88: 67-71. [DOI:10.1016/j.smallrumres.2009.11.005]
39. Yeh, F.C., R.C. Yang, T.B.j. Boyle, Z.H. Ye and J.X. Mao. 1997. PopGene, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
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