دوره 8، شماره 18 - ( زمستان 1396 )
جلد 8 شماره 18 صفحات 160-155 |
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Abangah E, Zandi M, Sanjabi M R, Hadi Tavatori M H. (2018). Investigating of Polymorphism in Intron 1 of Prolactin Receptor Gene in Shal Breed and Shal×Romanov Crossbreed using PCR-SSCP Technique. Res Anim Prod. 8(18), 155-160. doi:10.29252/rap.8.18.155
URL: http://rap.sanru.ac.ir/article-1-907-fa.html
URL: http://rap.sanru.ac.ir/article-1-907-fa.html
آبانگاه اسحق، زندی محمد، سنجابی محمد رضا، هادی تواتری محمد حسین. بررسی چند شکلی اینترون یک ژن گیرنده پرولاکتین در نژاد شال و آمیخته های شال و رومانوف با استفاده از روش PCR-SSCP
پژوهشهاي توليدات دامي 1396; 8 (18) :160-155 10.29252/rap.8.18.155
چکیده: (4009 مشاهده)
پرولاکتین از هیپوفیز قدامی ترشح می شود و نقشهای مهمی از جمله در توسعه پستان، تمایز و شیرواری دارد. هدف از این مطالعه ارزیابی چند شکلی ژن گیرنده پرولاکتین در نژاد شال و آمیختههای شال و رومانوف بود. برای این منظور پس از خونگیری از دامها و استخراج DNA به روش فنول کلروفرم، دو قطعه 176 و 215 جفت بازی از اینترون 1 ژن گیرنده پرولاکتین توسط آغازگرهای اختصاصی و طی واکنش PCR تکثیر شدند. سپس محصولات PCR با استفاده از الکتروفورز ژل آکریل آمید و رنگ آمیزی نقره مورد آنالیز قرار گرفتند. نتایج نشان داد در قطعه 176 جفت بازی از ژن گیرنده پرولاکتین (1PRLR) در نژاد شال دو ژنوتیپ AB و AC مشاهده شد. فراوانی این ژنوتیپها به ترتیب 92/0 و 08/0 و فراوانی آللهای A، B و C به ترتیب 50/0،46/0 و 04/0 بود. همچنین در جایگاه 1PRLR از این ژن در نژاد آمیخته شال و رومانف فراوانی ژنوتیپ AB و AC به ترتیب 94/0 و 06/0 مشاهده شد و فراوانی آللهای A، B و C نیز به ترتیب 50/0، 47/0 و 03/0 بود. بررسی چند شکلی در قطعه 215 جفت بازی از ژن گیرنده پرولاکتین (2PRLR) در نژاد شال و آمیخته شال و رومانف، تنها ژنوتیپ AA را نشان داد. نتایج این مطالعه نشان داد که جایگاه 1PRLR در نژاد شال و آمیخته شال و رومانف چند شکل در حالی که جایگاه 2PRLR در نمونههای مورد مطالعه یک شکل بود.
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