Volume 8, Number 15 (6-2017)                   rap 2017, 8(15): 115-123 | Back to browse issues page

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Design and Construction of Multiple-Epitope Recombinant Vaccine Against Foot-and-Mouth Disease Virus Type O . rap. 2017; 8 (15) :115-123
URL: http://rap.sanru.ac.ir/article-1-763-en.html

Abstract:   (183 Views)

     Foot-and-mouth disease (FMD) is an extremely infectious and occasionally fatal viral disease with a rapid onset and a short course that affects cloven-hoofed animals and results in considerable financial losses. For this reason, availability of more reliable and efficient vaccines against Foot-and-mouth disease virus (FMDV) is felt as a necessity. The purpose of this study is to develop a perfectly safe immunogen in order to supplant the usual inactivated vaccine. A tandem repeat multiple-epitope recombinant vaccine against foot-and-mouth disease (FMD) virus (FMDV) type O was developed. In order to develop recombinant epitope vaccines against foot-and-mouth disease virus (FMDV), multiple-epitope recombinant vaccine (rP) comprising various combinations of B cell and T cell epitopes from VP1 capsid protein were synthesized. A 1155-bp gene fragment was amplified by PCR using specific primers. The amplicon was purified and then cloned into expression vector pET-32a. For expression of recombinant protein, pET32a-rP plasmid was transformed into Escherichia coli BL21 (DE3) cells. Recombinant protein was overexpressed with isopropythio-beta-D-galctoside (IPTG). SDSPAGE and DOT blotting were performed for protein determination and verification.The multiple-epitope recombinant was validated by colony-PCR and enzymatic digestion. IPTG with a dosage of 1.0 mmol/L could effectively promote protein expression.  SDS-PAGE analysis demonstrated that our constructed prokaryotic expression system pET32a-rP efficiently produces a target recombinant protein with a molecular weight of about 60 kDa. Results showed  that prokaryotic expression system is effective in producing the multiple-epitope recombinant immunogen for FDM virus and can be used as a potential method for poly epitope constructs.

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Type of Study: Research | Subject: Special

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