Volume 8, Issue 15 (6-2017)                   rap 2017, 8(15): 115-123 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Design and Construction of Multiple-Epitope Recombinant Vaccine Against Foot-and-Mouth Disease Virus Type O . rap. 2017; 8 (15) :115-123
URL: http://rap.sanru.ac.ir/article-1-763-en.html
Abstract:   (1173 Views)

     Foot-and-mouth disease (FMD) is an extremely infectious and occasionally fatal viral disease with a rapid onset and a short course that affects cloven-hoofed animals and results in considerable financial losses. For this reason, availability of more reliable and efficient vaccines against Foot-and-mouth disease virus (FMDV) is felt as a necessity. The purpose of this study is to develop a perfectly safe immunogen in order to supplant the usual inactivated vaccine. A tandem repeat multiple-epitope recombinant vaccine against foot-and-mouth disease (FMD) virus (FMDV) type O was developed. In order to develop recombinant epitope vaccines against foot-and-mouth disease virus (FMDV), multiple-epitope recombinant vaccine (rP) comprising various combinations of B cell and T cell epitopes from VP1 capsid protein were synthesized. A 1155-bp gene fragment was amplified by PCR using specific primers. The amplicon was purified and then cloned into expression vector pET-32a. For expression of recombinant protein, pET32a-rP plasmid was transformed into Escherichia coli BL21 (DE3) cells. Recombinant protein was overexpressed with isopropythio-beta-D-galctoside (IPTG). SDSPAGE and DOT blotting were performed for protein determination and verification.The multiple-epitope recombinant was validated by colony-PCR and enzymatic digestion. IPTG with a dosage of 1.0 mmol/L could effectively promote protein expression.  SDS-PAGE analysis demonstrated that our constructed prokaryotic expression system pET32a-rP efficiently produces a target recombinant protein with a molecular weight of about 60 kDa. Results showed  that prokaryotic expression system is effective in producing the multiple-epitope recombinant immunogen for FDM virus and can be used as a potential method for poly epitope constructs.

Full-Text [PDF 570 kb]   (581 Downloads)    
Type of Study: Research | Subject: Special
Received: 2017/06/17 | Accepted: 2017/06/17 | Published: 2017/06/17

1. Abdul-Hamid, N.F., N.M. Hussein, J. Wadsworth, A.D. Radford, N.J. Knowles and D.P. King. 2011. Phytogeography of foot and mouth disease virus types O and A in malaysia and surrounding countries. Infection, Genetics and Evolution, 11: 320-328. [DOI:10.1016/j.meegid.2010.11.003]
2. Al-Shawkany, A., M. Nassiri, S. Zibaei, M. Tahmoorespour, S.M. Ziaratnia, M.F. Najafi, A.R. Haghparast, S. Ghovvati, S.H. Pourseyed and M. Rashtibaf. 2012. Amino acid diversity of antigenic sites of iranian type O foot-and-mouth disease virus. Journal of Cell and Molecular Research, 3: 66-74.
3. Bachrach, H.L. 1968. Foot-and-Mouth Disease. Annual Reviews in Microbiology, 22: 201-244. [DOI:10.1146/annurev.mi.22.100168.001221]
4. Bittle, J.L., R.A. Houghten, H. Alexander, T.M. Shinnick, J.G. Sutcliffe and R.A. Lerner. 1982. Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature, 298: 30-33. [DOI:10.1038/298030a0]
5. Brinkmann, U., R.E. Mattes and P. Buckel. 1989. High-level expression of recombinant genes in Escherichia Coli is dependent on the availability of the DNA Y gene product. Gene, 85: 109-114. [DOI:10.1016/0378-1119(89)90470-8]
6. Brown, F. 1992. New approaches to vaccination against foot-and-mouth disease. Vaccine 10: 1022-1026. [DOI:10.1016/0264-410X(92)90111-V]
7. Caron, L., M. Brum, M.P. Moraes, W.T. Golde, C.W. Arns and M.J. Grubman. 2005. Granulocyte-macrophage colony-stimulating factor does not increase the potency or efficacy of a foot-and-mouth disease virus subunit vaccine. Pesquisa Veterinária Brasileira, 25: 150-158. [DOI:10.1590/S0100-736X2005000300005]
8. DiMarchi, R., G. Brooke, C. Gale, V. Cracknell, T. Doel and N. Mowat. 1986. Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science, 232: 639-641. [DOI:10.1126/science.3008333]
9. Du, Y., Y. Li, H. He, J. Qi, W. Jiang, X. Wang, B. Tang, J. Cao, X. Wang and P. Jiang. 2008. Enhanced immunogenicity of multiple-epitopes of foot and mouth disease virus fused with porcine interferon α in mice and protective efficacy in guinea pigs and swine. Journal of Virological Methods, 149: 144-152. [DOI:10.1016/j.jviromet.2007.12.018]
10. Elmorjani, K., V. Lurquin, A. Lelion, H. Rogniaux and D. Marion. 2004. A bacterial expression system revisited for the recombinant production of cystine-rich plant lipid transfer proteins. Biochemical and Biophysical Research Communications, 316: 1202-1209. [DOI:10.1016/j.bbrc.2004.02.173]
11. Fang, M., J. Li, H. Wang, M. Yang, Y. Zhang, L. Zhou, H. Wei, G. Yang, Y. Yu and X. Wei. 2012. Correlation between efficacy and structure of recombinant epitope vaccines against bovine type O foot and mouth disease virus. Biotechnology letters, 34: 839-847. [DOI:10.1007/s10529-012-0856-0]
12. Gerner, W., M.S. Denyer, H.H. Takamatsu, T.E. Wileman, K.H. Wiesmüller, E. Pfaff and A. Saalmüller. 2006. Identification of novel foot-and-mouth disease virus specific T-cell epitopes in c/c and d/d haplotype miniature swine. Virus Research, 121: 223-228. [DOI:10.1016/j.virusres.2006.05.006]
13. W.N. P., S.K. Handelman, J.K. Everett, S.N. Tong, A. Bracic, J.D. Luff, V. Naumov, T. Acton, P. Manor and R. Xiao. 2011. Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli. . Microb Inform, 27:1(1):6 [DOI:10.1186/2042-5783-1-6]
14. King, A., B. Underwood, D. McCahon, J. Newman and F. Brown. 1981. Biochemical identification of viruses causing the 1981 outbreaks of foot and mouth disease in the UK. [DOI:10.1038/293479a0]
15. Li, G., W. Chen, W. Yan, K. Zhao, M. Liu, J. Zhang, L. Fei, Q. Xu, Z. Sheng and Y. Lu. 2004. Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or β-galactosidase as a carrier of immunogenic epitopes. Virology, 328: 274-281. [DOI:10.1016/j.virol.2004.07.025]
16. Liu, X.-S., Y.L. Wang, Y.G. Zhang, Y.Z. Fang, L. Pan, J.L. Lu, P. Zhou, Z.W. Zhang and S.T. Jiang. 2011. Identification of H-2d restricted T cell epitope of foot-and-mouth disease virus structural protein VP1. Virol Journal, 7: 426. [DOI:10.1186/1743-422X-8-426]
17. Mateu, M.G., J.A. Camarero, E. Giralt, D. Andreu and E. Domingo. 1995. Direct evaluation of the immunodominance of a major antigenic site of foot-and-mouth disease virus in a natural host. Virology, 206: 298-306. [DOI:10.1016/S0042-6822(95)80045-X]
18. Morgan, D. and D. Moore. 1990. Protection of cattle and swine against foot-and-mouth disease, using biosynthetic peptide vaccines. American Journal of Veterinary Research, 51: 40-45.
19. Rodriguez, L.L. and M.J. Grubman. 2009. Foot and mouth disease virus vaccines. Vaccine, 27: 90-94. [DOI:10.1016/j.vaccine.2009.08.039]
20. Shao, J.J., C.K. Wong, T. Lin, S.K. Lee, G.Z. Cong, F.W.Y. Sin, J.Z. Du, S.D. Gao, X.T. Liu and X.P. Cai. 2011. Promising multiple-epitope recombinant vaccine against foot-and-mouth disease virus type O in swine. Clinical and Vaccine Immunology, 18: 143-149. [DOI:10.1128/CVI.00236-10]
21. Sobrino, F., M. Sáiz, M.A. Jiménez-Clavero, J.I. Núñez, M.F. Rosas, E. Baranowski and V. Ley. 2001. Foot and mouth disease virus: a long known virus, but a current threat. Veterinary research, 32:1-30. [DOI:10.1051/vetres:2001106]
22. Song, H., L. Zhou, W. Fang, Y. Li, X. Wang, H. Fang, X. Li, M. Wu and B. Qiu. 2004. High-level expression of codon optimized foot-and-mouth disease virus complex epitopes and cholera toxin B subunit chimera in Hansenula polymorpha. Biochemical and Biophysical Research Communications, 315: 235-239. [DOI:10.1016/j.bbrc.2004.01.037]
23. Sutmoller, P., S.S. Barteling, R.C. Olascoaga and K.J. Sumption. 2003. Control and eradication of foot-and-mouth disease. Virus Research, 91: 101-144. [DOI:10.1016/S0168-1702(02)00262-9]
24. Upadhyay, A.K., A. Murmu, A. Singh and A. K. Panda. 2012. Kinetics of inclusion body formation and its correlation with the characteristics of protein aggregates in Escherichia coli. PLoS One, 7: e33951. [DOI:10.1371/journal.pone.0033951]
25. Zamorano, P., A. Wigdorovitz, M. Perez-Filgueira, C. Carrillo, J. Escribano, A. Sadir and M. Borca. 1995. A 10-Amino-Acid linear sequence of VP1 of foot and mouth disease virus containing B-and T-cell epitopes induces protection in mice. Virology, 212: 614-621. [DOI:10.1006/viro.1995.1519]
26. http://www.genscript.com/cgi-bin/tools/rare_codon_analysis
27. www.expasy.org/

Add your comments about this article : Your username or Email:

© 2019 All Rights Reserved | Research On Animal Production(Scientific and Research)

Designed & Developed by : Yektaweb