Research On Animal Production

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Rohipoor M, Nazari M, Beigi Nassiri M T. (2019). Genetic and Phylogenetic Analysis of Adani Goat Population Based on Cytochrome B Gene. rap. 10(26), 84-89. doi:10.29252/rap.10.26.84
URL: http://rap.sanru.ac.ir/article-1-994-en.html

Identification of genetic characteristics is an important factor for preservation of species life. The aim of this study was to identify the genetic characteristics of the Adani goat populations based on the cytochrome b (Cyt b) gene and to detec its phylogenetic relationships with the domestic and wild goat species using NCBI database. Blood samples were taken from 12 Adani goat and subsequently DNA was extracted using Sinaclon kit. The target area (892 base pair) was proliferated by specific primers using polymerase chain reaction (PCR) method and then sequenced. By analyzing the sequences, 5 haplotypes were identified based on 12 polymorphic sites. All mutation sites were transition and nonsense. The haplotype and nucleotide diversity and the average of a different nucleotide based on Cyt b region were estimated 0.57, 0.002 and 2.273, respectively. These results indicated high genetic variation in Adani goat. Using the NJ phylogenetic test results indicated that the Adani goat was located in the C. hircus branch and C. Aegagrus was in closer to them in compare with C. ibex. The placement of Capra hircus's goats and Adani goats in a similar branch is rational because of the dispersion of various races of this species throughout the world.