Volume 8, Issue 18 (3-2018)
Res Anim Prod 2018, 8(18): 76-82 |
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Ashrafi I, Daghigh Kia H, Moghaddam G, Saberivand A. (2018). New Formulation of Bull Semen Freezing Extender via Egg Yolk and Sodium Dodecyl Sulphate. Res Anim Prod. 8(18), 76-82. doi:10.29252/rap.8.18.76
URL: http://rap.sanru.ac.ir/article-1-897-en.html
URL: http://rap.sanru.ac.ir/article-1-897-en.html
Abstract: (4884 Views)
During the semen cryopreservation, various biochemical and anatomical compartments in the sperm cells may be altered. Therefore, the most important part of sperm-freezing protocols is use of cryoprotectant agents in freezing. The aim of this study was to determine the effect of different dosage of egg yolk (15, 20 and 25 %) as natural a cryoprotectant plus different dosage of sodium dodecyl sulphate (0, 0.05 and 0.1 %) as surfactant on quality cryopreserved Holstein bull sperm. In this study Semen was collected from three mature Holstein bulls twice a week using an artificial vagina and pooled ejaculates equally in order to eliminate the individual effects. After cooling, filling and sealing of the samples, they were frozen with nitrogen vapor and immersed in liquid. Results showed that semen extender with base of different dosage of egg yolk supplemented with various doses of SDS (0.05 and 0.1 %) increased (p < 0.05) total motility, progressive motility, linearity, lateral head displacement, straight linear velocity, curvilinear velocity, average path velocity, viability, integrity of the sperm membrane after the freeze–thawing process. Usage of 0.1% SDS with different dosage of egg yolk had higher value rather than 0.05% of SDS. whereas, there were no significant difference between 0.1% SDS +25% egg yolk and 0.1% SDS + 15% egg yolk, in conclusion, it could be suggested that the most effective combination of egg yolk and SDS in microscopic evaluations of the bull sperm freezing extender is 0.1% SDS + 15% egg yolk.
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