Volume 9, Issue 21 (11-2018)                   rap 2018, 9(21): 73-79 | Back to browse issues page

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Heydari S, Deldar H, Ansari Pirsaraei Z. Effect of Royal Jelly, Vitamin C and Vitamin E on Genes Expression of Antioxidant Enzymes in in vitro Maturation of Goat Oocytes . rap. 2018; 9 (21) :73-79
URL: http://rap.sanru.ac.ir/article-1-884-en.html
Abstract:   (607 Views)
The purpose of this study was to compare the antioxidant ability of royal jelly, vitamin E and vitamin C as additives to the maturation media on in vitro maturation of goat oocyte. To prepare goat oocytes, ovaries collected from local slaughterhouse were transported to the laboratory into the flask containing warm saline (30-34oC( in less than an hour. Cumulus oocyte complexs were removed from small antral follicles (2-6 mm) with slicing method, and were transferred to IVM medium. Cumulus oocyte complexs were put in maturation medium for 24 hours, and were reached to metaphase meiosis II (nuclear maturation). This study was performed in a completely randomized design, with six treatments included control, 10 mg/mL royal jelly, 200 µM of vitamin E, 100 µM of vitamin C, 200 µM of vitamin E + 100 µM of vitamin C and DMSO (as a solvent of vit E) in ten replicates. The results of this study showed that addition of royal jelly, vitamin E and vitamin C to the maturation medium, increased in vitro maturation rate of goat oocyte in comparison of control group. Supplementation of vitamin E, vitamin C and their combination significantly increased the oocyte meiotic maturation rate (87.26% ± 4.45) compared with the royal jelly (76.26% ± 2.55) and the control group (71.1% ± 1.26). Also, the addition of vitamin E + vitamin C combination was significantly increased relative gene expression of glutathione peroxidase but the superoxide dismutase and catalase were not affected by the treatment groups. In conclusion, the combination of vitamin C and vitamin E improved maturation rate and redox status of oocyte during in vitro maturation.
Full-Text [PDF 568 kb]   (222 Downloads)    
Type of Study: Research | Subject: فیزیولوژی
Received: 2018/02/14 | Revised: 2018/11/27 | Accepted: 2018/05/16 | Published: 2018/11/27

1. Barzegari Firozabadi, F. 2001. Effect of in vitro ascorbic acid and FSH on oocyte maturation and mouse follicles. Scientific Journal of Medical University shahid sadoghi yazd, 19: 586-597.
2. Mohammadi, S., H. Deldar and Z. Ansari pirsaraei. 2015. Effect of royal jelly on genes expression of antioxidant enzymes in in vitro maturation of goat oocytes. Master's thesis at Sari University of Agricultural Sciences and Natural Resources,
3. Shariatzade, S., M. Soleymani Mehranjani, A. Hamta and M. Ghandizade. 2012. Study the effect of vitamin E on the structure and number of ovarian follicles during ovarian development in rats treated with sodium arsenite (sterological examination). Arak University of Medical Sciences Scientific Journal, 15: 54-64.
4. Asadi, E., M. Jahanshahi and M.J. Golalipour. 2012. Effect of Vitamin E on Oocytes Apoptosis in Nicotine-Treated Mice. Iranian Journal of Basic Medical Science, 3: 880-884.
5. Cetica, P.L. and G. Pintos. 2002. Activity of key enzymes involved in glucose and triglyceride catabolism during bovine oocyte maturation in vitro. Reproduction, 124: 675-681. [DOI:10.1530/reprod/124.5.675]
6. Chow, C.K. 1991. Vitamin E and oxidative stress. Free Radical Biology and Medicine, 11: 215-232. [DOI:10.1016/0891-5849(91)90174-2]
7. Donnelly, E.T., N. McClure and S.E. Lewis. 1999. The effect of ascorbate and alpha-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa. Mutagenesis, 14: 505-512. [DOI:10.1093/mutage/14.5.505]
8. Elhassan, Y.M. and R.W. Wright. 1995. The effect of selenium and vitamin E addition on cleavage rate of IVM/IVF bovine oocytes. Theriogenology, 43: 206-273. [DOI:10.1016/0093-691X(95)92360-L]
9. Eshtiyaghi, M., H. Deldar and Z. Ansari pirsaraei. 2016. Royal jelly may improve the metabolism of glucose and redox state of ovin oocytes matured in vitro and emdryonic development following in vitro fertilization. Theriogenology, 86: 2210-2221. [DOI:10.1016/j.theriogenology.2016.07.019]
10. Guerin, P. and Y. Menezo. 1995. Hypotaurine and taurine in gamete and embryo environments: de novo synthesis via the cysteine sulfinic acid pathway in oviduct cells. Zygote, 3: 333-343. [DOI:10.1017/S0967199400002768]
11. Jamnik, P., D. Goranovic and P. Raspor. 2007. Antioxidative action of royal jelly in the yeast cell. Experimental Gerontology, 42: 594-600. [DOI:10.1016/j.exger.2007.02.002]
12. Kamakura, M., M. Maebuchi., S. Ozasa and T. Ogawa. 2005. Influence of royal jelly on mouse hepatic gene expression and safety assessment with a DNA microarray. Nutritional Science and Vitaminology, 51: 148-155. [DOI:10.3177/jnsv.51.148]
13. Kodama, H., R. Yamaguchi., J. Fukuda., H. Kasai and T. Tanaka. 1997. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertility and Sterility, 68: 519-524. [DOI:10.1016/S0015-0282(97)00236-7]
14. Olson, S.E. and G.E. Seidel. 2000. Culture of in vitro-produced bovine embryos with vitamin E improves development in vitro and after transfer to recipients. Biology of Reproduction, 62: 248-252. [DOI:10.1095/biolreprod62.2.248]
15. Valiollahpoor Amiri, M., H. Deldar and Z. Ansari Pirsaraei. 2016. Impact of supplementary royal jelly on in vitro maturation of sheep oocytes: genes involved in apoptpsis and embryonic development. System Biology in Reproductive Medicine, 62: 31-38. [DOI:10.3109/19396368.2015.1088102]
16. Fraga, C.G., P.A. Motchnik and M.K. Shigenaga. 1991. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proceedings of the National Academy of Sciences, 88: 11003-11006. [DOI:10.1073/pnas.88.24.11003]
17. Pavel, C.L., L.A.O. Marghitas, D.S. Bobis, A. Dezmirian, I. Sapcaliu and M.N. Madas. 2011. Biological activities of royal jelly. Animal Science and Biotechnology, 44: 108-118.
18. Pourmoradian, S., R. Mahdavi, M. Mobasseri, E. Faramarzi and M. Mobasseri. 2012. Effects of royal Jelly supplementation on body weight and dietary intake in type 2 diabetic females. Health Promotion Perspectives, 2: 231-235.
19. Guo, H., Y. Kouzuma and M. Yonekura. 2008. Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry, 113: 238-245. [DOI:10.1016/j.foodchem.2008.06.081]
20. Karadeniz, A., N. Simsek, E. Karakus, S. Yildirim, A. Kara, I. Can, F. Kisa, H. Emre and M. Turkeli. 2011. Royal Jelly Modulates Oxidative Stress and Apoptosis in Liver and Kidneys of Rats Treated with Cisplatin. Oxidative Medicine and Cellular Longevity, 10 pp. [DOI:10.1155/2011/981793]
21. Aslan, A., M. Cemek, M.E. Buyukokuroglu, K. Altunbas, O. Bas and Y. Yurumez. 2012. Royal jelly can diminish secondary neuronal damage after experimental spinal cord injury in rabbits. Food and Chemical Toxicology, 50: 2554-2559. [DOI:10.1016/j.fct.2012.04.018]
22. Martos, M., Y.R. Navajas, J.F. Lopez and J.A.P. Alvarez. 2008. Functional properties of honey, propolis, and royal jelly. Journal of Food Science, 73: 117-124. [DOI:10.1111/j.1750-3841.2008.00966.x]

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