Volume 9, Issue 21 (11-2018)                   rap 2018, 9(21): 105-112 | Back to browse issues page

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Ghazi Khani Shad A, Sharifi Shourabi M K. Study of the Polymorphism of Leptin Gene and Its Association with some Growth Traits in Lori Bakhtiari and Lori Bakhtiari-Afshari Croessbreed Sheep. rap. 2018; 9 (21) :105-112
URL: http://rap.sanru.ac.ir/article-1-831-en.html
Abstract:   (1052 Views)


The purpose of this study was evaluation of leptin gene polymorphism by PCR-SSCP and its relationship with some growth traits in Lori Bakhtiari and crossbred of Lori Bakhtiari- Afshari sheep. Blood samples were collected from 58 sheep (male and female) of Lori-Bakhtiari in Shahr-e-Kord Sholi station and 42 sheep (male and female) of Lori Bakhtiari-Afshari crossbreed from villages of Shahr-e-Kord. DNA was extracted, using extraction kit of Sinnagen co. Evaluation of quality and quantity of DNA was performed using agarose gel electrophoresis and spectrophotometry. Polymerase chain reaction (PCR) was conducted to amplify 275 bp fragment of exon 3 of leptin gene. Then single strand conformation polymorphism (SSCP) of PCR products was performed and genotypic patterns were obtained using acrylamid gel and silver staining. For leptin gene in Lori bakhtiari sheep, 8 band patterns including L1to L8 and for crossbreeds sheep, 10 band patterns including L1to L10 were obtained. There was a high polymorphism in exon 3 of the leptin gene in both breeds. Also results of means comparison showed that the leptin gene was significantly associated with weight at 6 and 12 months.

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Type of Study: Research | Subject: ژنتیک و اصلاح نژاد دام
Received: 2017/11/12 | Revised: 2018/11/27 | Accepted: 2018/07/17 | Published: 2018/11/27

1. Ahmadpanah, J. 2016. Phylogenetic analysis and molecular evolution of the leptin gene. Research on Animal Production, 7(13): 208-213. [DOI:10.18869/acadpub.rap.7.13.213]
2. Bahrenberg, G., I. Behrmann, A. Barthel and P. Hekerman. 2002. Indentifition of the critical sequence elements in the cytoplasmic domain of leptin receptor isoforms required for Janus kinas/ singal transducer and activator of transcription activation by receptor heterodimers. Molecular Endocrinology, 16(4): 859-872. [DOI:10.1210/mend.16.4.0800]
3. Boucher, D, M.F. Palin, F. Castonguay, C. Gariépy and F. Pothier. 2006. Detection of polymorphisms in the ovine leptine (LEP) gene: Association of a single nucleotide polymorphism with muscle growth and meat quality traits. Canadian Journal of Animal Science, 86: 31-35.
4. Buchanan, F.C., C.J. Fitzsimmons, A.G. Van Kessel, T.D. Thue, D.C. Winkelman Sim and S.M. Schmutz. 2002. Association of a missense mutation in the bovine Leptin gene with carcass fat content and leptin mRNA levels. Genetic Selection Evolution, 34: 105- 116. [DOI:10.1051/gse:2001006]
5. Buchanan, F.C, V. kessel and A.G. Waldner. 2003. Hot topic. An association between A Leptin single polymorphism and milk and protein yield, Journal of Dairy Science. 86: 3164-3166. [DOI:10.3168/jds.S0022-0302(03)73918-6]
6. Daridia, S., J. Williams, U. Bruggemand, M. Onagbesand, N. Raverc, E. Decuypred, J. Djianed, A. Gertlerc and M. Taouis. 2000. Achichen leptin -specific radio immunoassay. Domestic Animal Endocriology, 18: 325-335. [DOI:10.1016/S0739-7240(00)00053-9]
7. Friedman, J.M. and J.L. Halaas. 1998. Leptin and the regulation of body weight in mammals. Nature 395: 763-770. [DOI:10.1038/27376]
8. 8- Javanmard, A. 2001. Study the polymorphism of growth hormone receptor gene promotor (GHR) in Sistani cows. M.Sc. Dissertation in Animal Science. University of Tehran (In Persian).
9. Keshavarz, A. 2011. Study the polymorphism in Leptin gene in Najdi cows using PCR-RFLP teqnique. MSc Dissertation on Animal Science. Ramin University. 67 pp (In Persian).
10. Lagonigro, R.P., F. Wiener, J.A. Pilla and J. Woolliams. 2003. A new mutation in the coding region of the bovine Leptin gene associated with feed intake. Animal Genetics, 34(5): 371-374. [DOI:10.1046/j.1365-2052.2003.01028.x]
11. Liefers, S.C., M.F. Te Pas, R.F. Veerkamp, Y. Chilliard, C. Delavaud and R. Gerritsen. 2003. Association of Leptin gene polymorphisms with serum leptin concentration in dairy cows. Mammalian Genome, 14: 657-663. [DOI:10.1007/s00335-003-2275-y]
12. Matarese, G., A. La Cava, V. Sanna, GM. Lord, R.I. Lechler and S. Fontana. 2002. Balancing susceptibility to infection and autoimmunity: a role for leptin. Trends in Immunology, 23:182-187.1 [DOI:10.1016/S1471-4906(02)02188-9]
13. Schenkel, F.S., S.P. Miller, X.Ye.S. Moores, J.D. Nkrumah and C. Li. 2005. Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle. Journal of Animal Science, 83: 2009-2020. [DOI:10.2527/2005.8392009x]
14. Shojaei, M., M.R. Mohammadabadi, M. Asadi Fozi, K.A. Esmailizadeh, M.H. Ferdowsi, A. Torabi, M. Tayyarzadeh and H. Mirzakhani. 2010. Using PCR-SSCP technique to investigate polymorphism of Leptin gene in Kermani sheep. Animal Science Researches, 20: 115-122.
15. Solgi, H., A. Tohidi and M. Moeini. 2004. Relationship of Leptin concentration and FSH with lambing percent in Iranian sheep. Proceeding of First Congress on Animal Aquatic Science. University of Tehran. 31 August- 2 September (In Persian).
16. Shibak, A., B. Montazertorbati, H. Farhangfar, R. Ashkani Far. 2014. Study of genetic polymorphisms in exon 3 of the Leptin gene and its assosiation with birth weight and growth traits in North Khorasan Kurdi sheep. Modern Genetic, 4: 511-516 (In Persian).
17. Talebi, M.A., S.R. Miraei Ashtiani, M. Moradi Shahre Babak and A. Nejati Javaremi. 2010. Econimic values for reproduction, growth and carcass in Lori Bakhtiari Sheep. Animal Science Journal, 41(13) 203-213 (In Persian).
18. Tahmoorespoor, M., M.R. Nasiri, M. Ansari, A. Heravi and F. Eftekhar Shahroodi. 2008. Polymorphism of Leptin gene and its relationship with daily dain in Baloochi sheep. Proceeding of 3th Congress on Animal Aquatic Science. University of Ferdoosi. Mashhad (In Persian).
19. Tahmures poor, M., M.R. Nassiry, M. Ansary, A. Heravi Mousavi, M. Vafaye vale and F. Eftekhar Shahroudi. 2009. Analysis of Leptin genepolymorphism and their association with average dailygain trait in Baluchi sheep. 3rd Congress of animal science, Mashhad, Iran.
20. Taouis, M., J.W. Chen, C. Daviaud, J. Dupont, M. Derouet and J. Simon. 1998. Clonning the chiken Leptin gene. Gene, 208: 239-242. [DOI:10.1016/S0378-1119(97)00670-7]
21. Van der Lende, T., M.F. Te Pas, R.F. Veerkamp and S. Liefers. 2005. Leptin gene polymorphisms and their phenotypic associations. Vitamins and Hormones, 71: 373-404. [DOI:10.1016/S0083-6729(05)71013-X]
22. Vatan Khah, M., M. Moradi Shahr Babak, A. Nejati Javaremi, R. Vaez Torshizi and S.R. Miraei Ashtiani. 2005. Study the phenotypic and genetic characterizations for growth traits in Lori Bakhtiari lambs. Iranian Agricultural Science Journal, 36(6): 1455-1463 (In Persian).
23. Yang, D., H. Chen, H.X. Wang, Zh. Tian and L. Tang. 2007. Association of polymorphisms of leptin gene with body weight and body sizes indexes in Chinese indigenous cattle. Journal of Genetics and Genomics, 34(5): 400-405. [DOI:10.1016/S1673-8527(07)60043-5]
24. Zhang, Y., R. Proenca, M. Maffei, M. Barone, L. Leopold, J.M. Friedman.1994. Positional cloning of the mouse obese gene and its human homologue. Nature, 372: 425-432 [DOI:10.1038/372425a0]
25. Zhou, H., J.G. Hickford and H. Gong. 2009. Identification of allelic polymorphism in the ovine leptin gene. Mol Biotechnology, 41: 22-25. [DOI:10.1007/s12033-008-9090-3]

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