Volume 8, Issue 15 (6-2017)                   rap 2017, 8(15): 105-114 | Back to browse issues page


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The Effect of Different Levels of Levamisole Drug on Performance and Related Parameters with Immune System in Broiler Chickens. rap. 2017; 8 (15) :105-114
URL: http://rap.sanru.ac.ir/article-1-760-en.html
Abstract:   (1419 Views)

     This experiment was conducted to study the effect of different levels of levamisole on performance and related parameters with immune system in Cobb 500 strain male broilers. The experiment was carried out a completely randomized design, with 300 male broiler chickens in four treatments and five replicates 15 birds/replicates. The experimental groups consisted of zero, 2.5, 5 and 7.5 mg per kg live body weight of levamisole in the third week. Parameters examined include performance and related parameters with immune system, phagocytic activity test, antibody titers against Newcastle, Influenza and Gambro disease and organs weight involved in immune system. Results showed that adding of levamisole into diet had no effect on body weight gain, feed intake and feed conversion ratio in broilers. Adding of levamisole into diet increased the number of lymphocytes, monocytes and heterophils in broiler (P<0.05). Levamisole improved cellular immune system in broiler chickens (P<0.05). Phagocytic activity was increased in broilers treated with levamisole at 7.5 kg per kg of live body weight (P<0.05). Use of levamisole in broiler chickens increased antibody titers than controls, but this increase was not significant at 28 days except against Gambro disease. At 42 days, Levamisole had a significant effect on the size of the bursa Fabricius in broilers (P<0.05). Also, there were no mortalities in any of the experimental treatments. It seems, levamisole may have a beneficial effect on the immune system of broiler chickens. Between three different levels of levamisole, the levels of 5 and 7.5 mg per kg of live body weight had better resultion parameters involved in immune system of broiler chicks.

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Type of Study: Research | Subject: Special
Received: 2017/06/17 | Revised: 2017/06/19 | Accepted: 2017/06/17 | Published: 2017/06/17

References
1. Amery, W.K., F. Spreafico, .A.E Rojas, .F Denissen and M. Chingos. 1978. Adjuvant treatment with levamisole in cancer. A review of experimental and clinical data. Cancer Treatment Reviews, 4: 167-194. [DOI:10.1016/S0305-7372(77)80023-6]
2. Cazella, L.N., P.E. Pardo and N. Frazatti-Gallina. 2009. Effect of levamisole on the humoral response against rabies in cattle. Veterinary Record, 165: 722-723.
3. Cuesta, A., M . Esteban and J. Meseguer. 2002. Levamisole is a potent enhancer of gilthead seabream natural cytotoxic activity. Veterinary Immunology and Immunopathology, 89: 169-174. [DOI:10.1016/S0165-2427(02)00220-9]
4. Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42. [DOI:10.2307/3001478]
5. El-Kholy, H. and B.W. Kemppainen. 2005. Levamisole residues in chicken tissues and eggs. Poultry Science, 84:9-13. [DOI:10.1093/ps/84.1.9]
6. Euseby J.P. 1986. Proprietes immunos timulantes du levamisole. Revue de Medecine Veterinaire, 137: 417-426.
7. Fathi, E., M. Bozorgmehrifard, M. Ahangaran and Kh. Kamali. 2009. The effect of levamisole hydrochloride on changes of lymphocytes and serum antibody against Newcastle virus in broiler chickens. Veterinary Journal, 3: 32-36 (In Persian).
8. Funde, S.T. 2005. Effect of Ocimum sanctum, Emblica officinalis and Levamisole on immune response in immunosuppressed broilers. M.V.Sc. Thesis submitted to M.A.F.S.U., Nagpur.
9. Giambrone, J.J. and P.H. Klesius. 1985. Effect of levamisole on the response of broilers to coccidiasis vaccination on poultry. Poultry Science, 64: 1083-1089. [DOI:10.3382/ps.0641083]
10. Gomi, K., M. Morimoto and K. Nomoto. 1982. Cytotoxic T-Cell-mediated antitumor effect of levamisole against murine syngeneic fibrosarcoma. Journal of Cancer Research, 42: 4197-4202.
11. Gwilt, P., M. Tempero, A. Kremer, M. Connolly and C. Ding. 2000. Pharmacokinetics of levamisole in cancer patients treated with 5-fluorouracil. Cancer Chemotherapy and Pharmacology, 45: 247-251. [DOI:10.1007/s002800050036]
12. Habibi, M., M.A. Zandieh, H. Ghahri, R. Salmanzade and F. Yeganeh. 2012. Effects of levamisole on the immune response of broilers against Newcastle disease vaccines. African Journal of Pharmacy and Pharmacology, 6: 1860-1864. [DOI:10.5897/AJPP12.294]
13. Hardman, J.G., I.E. Limberd and A.G. Gilman. 2001. The pharmacological basis of therapeutics, 10th Ed. McGraw Hill. New York. pp: 1477.
14. Heller, E.D., G. Leitner, A. Friedman, Z. Uni, M. Gutman and A. Cahaner. 1992. Immunological parameters in meat-type chicken lines divergently selected by antibody response to Escherichia coli vaccination. Veterinary Immunology and Immunopathology, 34: 159-172. [DOI:10.1016/0165-2427(92)90159-N]
15. Hersey, P. and J. Werkmeister. 1981. Inhibition of suppressor T cells in pokeweed mitogen stimulated cultures of T and B cells by levamisole in vitro and in vivo. Clinical and Experimental Immunology, 46: 340-349.
16. Jin, H., Y. Li, Z. Ma, F. Zhang, Q. Xie, D. Gu and D. Wang. 2004. Effect of chemical adjuvants on DNA vaccination. Vaccine, 22: 2925-2935. [DOI:10.1016/j.vaccine.2003.12.026]
17. Kang ,Y., H. Jin, G. Zheng, Q. Xie, J. Yin, Y. Yu, C. Xiao, X. Zhang, A. Chen and B. Wang. 2005. The adjuvant effect of levamisole on killed viral vaccines. Vaccine, 23: 5543-5550. [DOI:10.1016/j.vaccine.2005.07.017]
18. Katzung, B. 2001. Basic and clinical pharmacology, 8Th Ed. McGraw Hill. New York. pp: 979.
19. Khalegi Miran, S.N., M.A. Karimi Torshizi, M.R. Bassami and H. Jandaghi. 2010. Effect of three immunostimulants on some of indicators of broiler immune response. Japan Poultry Science, 47: 321-325. [DOI:10.2141/jpsa.009114]
20. Kulkarni, V.B., A.N. Mulbagal, V.L. Paranjape, J.B. Khot and A.V. Manda. 1973. Immunostimulating effect of Tetramisole on antibody formation against Newcastle disease virus in chicks. Indian Veterinary Journal, 50: 225-227.
21. Lai, W.H., S.Y. Lu and H.L. Eng. 2002. Levamisole aids in treatment of refractory oral candidiasis in two patients with thymoma associated with myasthenia gravis: report of two cases. Chang Gung Medical Journal, 25: 606-611.
22. Li, P., Y. Yin, D. Li, S.W. Kim and G. Wu. 2007. Amino acids and immune function. British Journal Nutrition, 98: 237-252. [DOI:10.1017/S000711450769936X]
23. Mani, K., K. Sundarsan and K. Vishwanathan. 2001. Effect of immunomodulators on performance of broilers in aflatoxicosis. Indian Veterinary Journal, 78: 1126-1129.
24. Mayahi, M., M.R. Seifiabad Shapouri and F. Talazadeh. 2007. Effect of levamisole administration on humoral immune response against influenza vaccine in broiler chickens. Iranian Veterinary Journal, 3: 89-94 (In Persian).
25. Munir, K., M.A. Muneer, A. Tiwari, E. Masaoud and R.M. Chaudhry. 2009. Effects of salinomycin on cell-mediated immunity of broiler chickens against hydro pericardium syndrome and Newcastle disease viruses. Poultry Science, 88: 86-91. [DOI:10.3382/ps.2008-00345]
26. Padmavathi, P., S.R.G. Muralidharan and S. Krishnaswamy. 1988. A study of the effect of Levamisole on Eimeria tenella experimental infection. Indian Veterinary Journal, 65: 872-874.
27. Panda, B.K. and S.K. Dwivdi. 1995. Use of some noncoccidial agents in experimentally induced coccidiosis in Japanese quails (from Hydrabad), India Journal of Veterinary Medicine, 15: 15-13.
28. Panda B.K., S.K. Sahoo and M.K. Padhi. 2004. Comparative studies on immunostimulating effect of ImmuPlus, Levamisole and Vitamins (E and C) against Ranikhet disease in broilers. Indian Journal of Poultry Science, 39: 252-255.
29. Porchezhian, T. and N. Punniamurthy. 2006. Effect of oral levamisole hydrochloride on feed intake and body weight of broiler chicks. Journal of Animal and Veterinary Advances, 5: 847-848.
30. Rajput, A.B., B.R. Kolte, J.M. Shisodiya, J.M. Chandankhede and J.M. Chahande. 2009. Effect of vitamin A, vitamin C, vitamin E and levamisole on performance of broilers. Veterinary World, 2: 225-227.
31. Ramteke, B.R., N.P. Dakshinkar and A.V. Pandit. 1992. Senility field isolates of E. tenella to anticoccidial drugs in chickens. Poultry Adviser, 25: 69-73.
32. Renoux, G. 1971. Immunostimulating effect of an imidotiiazole in the immunization of mice against Brucella abortus infection. Comptes Rendus Hebdomadaires des Séances de l'Académie des Sciences, 272: 349-350.
33. Reyero, C., W. Stockl and G. Thalhammer. 1979. Stimulation of the antibody response to sheep red blood cells in piglets and young pigs by levamisole. Journal British Veterinary, 135: 17-24. [DOI:10.1016/S0007-1935(17)32983-4]
34. Rezayi, M. and A. Mokhtarzadeh. 2012. The effect of levamisole hydrochloride on the humoral immune response in broiler chickens vaccinated against Gambro. Proceedings of the 3rd International Veterinary Poultry Congress, February 22-23, Tehran, Iran (In Persian).
35. Roostaei Ali Mehr, M., M. Haghighian Roudsari, B. Mansori and G.R. Nikbakht Broujeni. 2012. Effects of oral levamisole hydrochloride on cellular and humoral immune responses in broiler chickens. Journal of Veterinary Research, 67: 235-241 (In Persian).
36. Sampson, D. and A. Lui. 1976. The effect of levamisole on cell-mediated immunity and suppressor cell function. Journal of Cancer Research, 36: 952-955.
37. SAS Institute. 2003. SAS User's Guide. Statistics. Version 9.1 ed. SAS Institute Inc., Cary, NC.
38. Soppi, E., O. Lassila, M.K. Viljanen, O.P. Lehtonen and J. Eskola. 1979. In vivo effect of levamisole on cellular and humoral immunity in normal chickens. Clinical and Experimental Immunology, 38: 609-614.
39. Soppi, E., M. Lassila, O. Viljanen, P. Lehtonen and J. Eskola. 1999. In vivo effect of levamisole on cellular and humoral immunity in normal chickens. Clinical and Experimental Immunology, 38: 609-614.
40. Stevenson, H.C., I. Green, J.M. Hamilton, B.A. Calabro and D.R. Parkinson. 1991. Levamisole known effects on the immune system, clinical results and future applications to the treatment of cancer. Journal of Clinical Oncology, 9: 2052-2066. [DOI:10.1200/JCO.1991.9.11.2052]
41. Symoens, J. and M. Rosenthal. 1977. Levamisole in the modulation of the immune response: The current experimental and clinical state. Journal of the Reticuloendothelial Society, 21: 175.
42. Szeleszczuk, P., E. Karpinska, W. Bielecki, W. Borzemska and G. Kosowska. 2003. Evaluation of chosen immune-modulators toxicity for chicken embryos and one-day-old chicks. Bulletin of the Veterinary Institute in Pulawy, 47: 411-417.
43. Tekade, S.H., S.G. Mode and S.P. Waghmare. 2008. Effect of Asparagus racemosus, Sida cordifolia and levamisole on immunological parameters in experimentally induced immunosuppressed broilers. Veterinary World, 1: 49-50.
44. Wauwe, J.V. and P.A.J. Janssen. 1991. On the biochemical mode of action of levamisole: an update. International Journal of Immunopharmacology, 13: 3-9. [DOI:10.1016/0192-0561(91)90019-4]

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