Volume 2, Issue 3 (5-2011)
rap 2011, 2(3): 49-58 |
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Khani M, Daghigh kia H, Zare-Shahneh A, Alijani S. (2011). Effect of Retinoic Acid on in Vitro Maturation of Ovine Immature Oocytes Containing Cumulus Cells
. rap. 2(3), 49-58.
URL: http://rap.sanru.ac.ir/article-1-137-en.html
URL: http://rap.sanru.ac.ir/article-1-137-en.html
Collage of Animal Science, University of Tabriz
Abstract: (4891 Views)
This study was carried out to investigate the effect of all-trans retinoic acid (t-RA) on in vitro maturation of ovine immature oocytes containing cumulus cells. The Oocytes with cumulus cells were recovered from follicles (2-6 mm in diameter). Different concentrations of t-RA (0, 1, 1.5 and 2 μmol) were added in the maturation medium. For oocytes maturation, all groups were located in the CO2 incubator for 24 hours. The meiosis and nuclear maturation status of the oocytes in each experimental group were assessed using an invert microscope and the data were analyzed with GLM procedure. There are not any oocytes in GV stage. The percentage of oocytes in GVBD stage at control group in comparison with 1 μmol and 2 μmol treatments was significant (p<0.05). The rate of oocytes maturation in 2 μmol retinoic acid treatments were significantly more than other groups (P<0.05). In conclusion, the use of 2 μmol retinoic acid increased the ovine oocyte maturation significantly in the commercial TCM199 medium.
Type of Study: Research |
Subject:
Special
Received: 2013/04/21 | Revised: 2019/02/19 | Accepted: 2013/04/29 | Published: 2013/04/29
Received: 2013/04/21 | Revised: 2019/02/19 | Accepted: 2013/04/29 | Published: 2013/04/29
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