Volume 2, Issue 4 (4-2011)                   rap 2011, 2(4): 35-47 | Back to browse issues page

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Zhandi­ M, Towhidi A, Zare-Shahneh A, Khodaei Motlagh M, Deldar H, Akhlaghi A. Effect of Insulin Like Growth Factor-1 on Developmental Competence of Bovine Embryos Produced From Heat-Stressed Oocytes in in vitro Culture Medium. rap. 2011; 2 (4) :35-47
URL: http://rap.sanru.ac.ir/article-1-129-en.html
University of Tehran
Abstract:   (2118 Views)

     The goal of this study was to assess the effect of Insulin like growth factor-1 (IGF-1) during in vitro culture on developmental competence of bovine embryos produced from heat-stressed oocytes. In this experiment, immatured oocytes were randomly assigned into two groups and matured at 38.5 or 41 °C for the first 12 hours of maturation followed by 12 hours at 38.5 °C. After in vitro maturation and fertilization, putative zygotes in each group were randomly allocated and cultured in the presence or absence of 100 ng/ml human recombinant (hr)-IGF-1. The results showed that cleavage rate was not affected by IGF-1 and heat stress, whereas blastocyst formation rate was significantly increased by IGF-1 in the presence and/or absence of heat stress. In addition, heat stress decreased blastocyst formation rate compared to the control
(38.5 °C without IGF-1) group. The number of trophectoderm cells, inner cell mass and total cell number were significantly increased by IGF-1 in the presence and/or absence of heat stress. In addition, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive nuclei were significantly decreased by IGF-1 in the presence and/or absence of heat stress. Results of this experiment demonstrated that IGF-1 can improve developmental competence of bovine embryos produced from heat-stressed oocytes. It seems that IGF-1 improve bovine embryo developmental competence via increasing of total cell number and decreasing of apoptosis.
 
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Type of Study: Research | Subject: Special
Received: 2013/04/21 | Revised: 2019/02/19 | Accepted: 2013/04/29 | Published: 2013/04/29

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