fa تأثیر مکمل سازی رقیق‌کننده با سطوح مختلف ال-کارنیتین بر کیفیت منی قوچ قزل بعد از فرآیند انجماد یخ گشایی در خارج فصل تولیدمثلی فیزیولوژی فیزیولوژی پژوهشي Research <p dir="RTL"><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">این</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تحقیق</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">باهدف</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بررسی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">اثر</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">آنتی‌اکسیدانی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">سطوح</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مختلف</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ال-کارنیتین بر</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">فراسنجه‌های</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">اسپرم</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">قوچ</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">پس</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">از فرآیند</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">انجماد</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">-</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">یخ گشایی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">انجام</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شد. نمونه‌ های</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">منی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">قوچ</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">پس</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">از</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">جمع‌آوری،</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">با</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">رقیق‌ کننده</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تریس-لسیتین</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">همراه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">غلظت‌های</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مختلف</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">35، 70، 105 میلی</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> مولار </span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در میلی&shy; لیتر</span></span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ال-کارنیتین رقیق‌شده و تا 5 درجه سانتی‌گراد به مدت 2 ساعت سرد شده و سپس</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">معرض</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بخار</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ازت</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">منجمد کرده و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">درنهایت</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تانک</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ازت</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مایع</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ذخیره</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شدند. پس از یخ&shy;گشایی صفات حرکتی، زنده‌مانی، مورفولوژی، یکپارچگی غشاء و مقدار مالون&shy;دی آلدئید مورد ارزیابی قرار گرفتند. بیشترین میزان تحرک کل و حرکت پیش&shy;رونده، در محیط حاوی 105 </span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">میلی</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مولار </span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در میلی&shy; لیتر</span></span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ال-کارنیتین مشاهده شد </span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ولی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نسبت به گروه شاهد تفاوت معنی‌ داری نداشت. افزودن ال-کارنیتین باعث افزایش یکپارچگی غشاء و کاهش معنی&shy; دار درصد اسپرم&shy;های غیرطبیعی نسبت به گروه شاهد نشد. همچنین افزودن 105 میلی</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مولار در میلی &shy;لیتر از ال-کارنیتین باعث افزایش معنی &shy;دار درصد زنده‌مانی و کاهش معنی‌ دار میزان مالون&shy;دی آلدئید تولیدی اسپرم &shy;ها نسبت به گروه 35 میلی&shy; مولار شد </span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">(05/0></span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:8.0pt;">p</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">).</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به‌طورکلی می&shy;توان نتیجه گرفت که</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">استفاده</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">از</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ال-کارنیتین در رقیق‌کننده منی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">قوچ قزل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">سبب</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بهبود</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">برخی فراسنجه &shy;های</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">اسپرم و کاهش اثرات منفی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ناشی از</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">فرآیند</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">انجماد-یخ گشایی نمی&shy; شود.</span></span></strong><br> &nbsp;<br> <br> &nbsp;</p> <p>The objective of this study was to investigate the effect of different levels of L-Carnitine on <em>Ghezel</em> ram semen characteristics after freeze-thawing. Semen samples were collected from 5 healthy and mature rams using an artificial vagina. Different concentrations of 35, 70, 105, mM L-Carnitine were diluted with lecithin-based semen extender and cooled to 5&deg;C and then placed in liquid nitrogen vapor and finally were stored in liquid nitrogen tank. The experiment was carried out on the basis of completely randomized design with 5 replicates. The results showed that the highest total motility and progressive motility were observed in groups containing 70 and 105 mM L-Carnitine that were not significantly different from the control group. The lowest motility recorded in this experiment was the first level of L-Carnitine. In other parameters only VAP, ALH, VCL and LIN were significantly different from the control group. The first level of L-Carnitin was statistically significant in morphology compared to the control group. Addition of 105 mM of L-Carnitine caused higher membrane integrity and lower MDA level compared to 35 mM group (p<0.05). MDA was not significant at any level. Therefore, supplementation of L-Carnitine in lecithin-based semen extender can improve semen quality of <em>Ghezel</em> ram and reduce negative effects of freeze-thawing process.<br> &nbsp;</p> ال-کارنیتین, انجماد-یخگشایی, اسپرم, قوچ Freeze-Thawing, L-Carnitin, Ram, Sperm 48 53 http://rap.sanru.ac.ir/browse.php?a_code=A-10-294-2&slc_lang=fa&sid=1 Hossein Daghigh Kia حسین دقیق کیا daghighkia@tabrizu.ac.ir 10031947532846008927 10031947532846008927 Yes University of Tabriz دانشگاه تبریز Sepehr Jafari سپهر جعفری sepehrjafari8@gmail.com 10031947532846008928 10031947532846008928 No University of Tabriz دانشگاه تبریز Gholamali Moghaddam غلامعلی مقدم ghmoghaddam@hotmail.com 10031947532846008929 10031947532846008929 No University of Tabriz دانشگاه تبریز Marziyeh Ebrahimi مرضیه ابراهیمی mrzh_ebrah@yahoo.com 10031947532846008930 10031947532846008930 No University of Tabriz دانشگاه تبریز Abozar Najafi ابوذر نجفی abozar.najafi@yahoo.com 10031947532846008931 10031947532846008931 No University of Tabriz دانشگاه تبریز