fa چندشکلی واریانت های G1 و B4 ژن‌های GDF9 و BMP15 در گوسفند دالاق تخصصي Special پژوهشي Research <strong><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">در سال&shy;های اخیر جهش&shy;هایی با اثرات عمده روی نرخ تخمک&shy;ریزی در دو جایگاه ژنی مربوط به لیگاند&shy;های </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">TGF&beta;</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> و نیز گیرنده&shy;های مربوط به </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">TGF&beta;</span><span dir="rtl"></span></strong><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><strong> شناسایی شده &shy;است. این مطالعه برای شناسایی چند&shy;شکلی&shy;های مربوط به لیگاندهای </strong></span><strong><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">BMP15</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> (واریانت </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">B4</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span>) و </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">GDF9</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> (واریانت</span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">G1</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span>) در گوسفند دالاق انجام شد. 100 نمونه خون از دو گله در سطح شهرستان گنبد کاووس جمع_&shy;آوری شد و استخراج </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">DNA</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> <span lang="FA"><font face="B Nazanin" size="3">به روش بهینه یافته نمکی انجام گرفت. با استفاده از دو جفت آغازگر اختصاصی قطعات 153 و 462<span style="mso-spacerun: yes">&nbsp; </span>جفت بازی از اگزون 2 ژن</font></span></span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">BMP15 </span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><font size="3"><font face="B Nazanin"><span style="mso-spacerun: yes">&nbsp;</span>و نیز اگزون 1 ژن </font></font></span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">GDF9</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> <span lang="FA"><font face="B Nazanin" size="3">توسط واکنش&shy;های زنجیره&shy;ای پلیمراز تکثیر انجام گرفت. هضم محصولات</font></span></span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">PCR </span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><font size="3"><font face="B Nazanin"><span style="mso-spacerun: yes">&nbsp;</span>به ترتیب با استفاده از آنزیم&shy;های برشی </font></font></span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">DdeI</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> و </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">HhaI</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> انجام شد. نتایج حاصل از هضم محصولات </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">PCR</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> وجود جهش در جایگاه </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">B4</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> را تأیید نکرد در حالیکه در جایگاه </span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">G1</span><span dir="rtl"></span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> چند</span><span 10pt="" 12.0pt="" b="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">&shy;</span><span 10.0pt="" 12pt="" b="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" roman="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">شکلی مشاهده شد. فراوانی ژنوتیپ هتروزایگوت در جمعیت مورد مطالعه برابر با 15% برآورد شد.</span></strong> <span 12pt="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-no-proof:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">Recently, mutations with major influence on ovulation rate and litter size were identified in </span><span 12pt="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">TGF&beta;<font size="3"><font face="Times New Roman"><span style="mso-no-proof: yes"> superfamily ligands and receptors. This </span>study was conducted to identify polymorphisms in GDF9 and BMP15 genes in Dalagh sheep breed. One hundred mature ewes from two flocks in Golestan province were genotyped for the GDF9 (G1) and BMP15 (B4) variants. Two fragments of 462bp and 153bp of GDF9 and BMP15 genes were amplified by PCR-RFLP method. PCR product was digested using HhaI and DdeI endonuclease restriction enzymes.</font></font></span><span 10pt="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">&nbsp;</span><span 12pt="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">The results of the digested PCR products, did not confirm mutation at position B4 but Polymorphism was observed at G1position. The heterozygote genotype frequency in the population was estimated at 15%</span><span 10pt="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">.</span> گوسفند دالاق, GDF9, BMP15, دوقلوزایی Dalagh sheep, GDF9, BMP15, Litter size 148 155 http://rap.sanru.ac.ir/browse.php?a_code=A-10-1-91&slc_lang=fa&sid=1 Alireza Khanahmadi علیرضا خان احمدی 100319475328460010174 100319475328460010174 Yes University of Gonbad Kavus دانشگاه گنبد کاووس Shahabeddin Gharehveysi شهابدین قره ویسی 100319475328460010175 100319475328460010175 No Islamic Azad University, Ghaemshahr Branch دانشگاه آزاد اسلامی واحد قائم شهر Rasul Khataminejad رسول خاتمی نژاد 100319475328460010176 100319475328460010176 No University of Gonbad Kavus دانشگاه گنبد کاووس Jalil Arab جلیل عرب 100319475328460010177 100319475328460010177 No Islamic Azad University, Ghaemshahr Branch دانشگاه آزاد اسلامی واحد قائم شهر