Volume 8, Issue 17 (1-2018)                   rap 2018, 8(17): 99-106 | Back to browse issues page


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(2018). Study of Candidate Genes Affecting Fat-Tail Traits in Specific Region of Chromosome 5 in Lori-Bakhtiari and Zel Sheep by PCR-SSCP . rap. 8(17), 99-106. doi:10.29252/rap.8.17.99
URL: http://rap.sanru.ac.ir/article-1-872-en.html
Abstract:   (3576 Views)
     Meat quality and carcass composition are the most important economic traits in sheep. Body fat and fat-tail are key factors affecting carcass quality and meat production in each breed, and mutations in sequences of the genes that control these traits can affect the animal performance and therefore the breeding value. The aim of this study was to investigate the polymorphism in some regions of SAR1B, SEC24A and VDAC1 genes in candidate genomic regions for fat traits on ovine chromosome 5. Blood samples were collected randomly from 300 Lori-Bakhtiari and 100 Mazandaran sheep and DNA was extracted using modified salting out method. Polymerase chain reaction (PCR) was performed for amplification of 478, 579 and 348 bp fragments of studied genes using specific primer pairs and genotyping of samples was done by SSCP technique. The obtained results showed three banding patterns of A, B and C for SAR1B marker site in Lori-Bakhtiari sheep but it was monomorph in Zel sheep. For SEC24 gene, three banding patterns of A, B and C were observed in both sheep breeds, but VDAC1 was monomorph in both sheep breed. The effect of banding patterns of SAR1B gene on fat-tail characteristics were significant (P<0.001), so that the A banding pattern showed highest measurement of the upper and lower, height and weight of fat-tail. The band patterns of SEC24A gene significantly associated with fat-tail characteristics, and banding pattern of B showed highest values. Considering the monomorphic pattern of SAR1B gene in Zel sheep and polymorphic pattern in fat-tailed Lori-Bakhtiary sheep, and also polyomorphic patterns of SEC24A gene in both breeds, these two marker site could be considered as potential candidate genes affecting fat tail characteristic in fat-tailed sheep breeds. More studies on different parts of these genes (exon, intron, regulatory regions) and their association with fat-tail traits is recommended for confirmation if the results.
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Type of Study: Research | Subject: Special
Received: 2018/01/10 | Revised: 2018/01/28 | Accepted: 2018/01/10 | Published: 2018/01/10

References
1. Archibald, A.L., N.E. Cockett, B.P. Dalrymple, T. Faraut, J.W. Kijas, J.F. Maddox, J.C. McEwan, V.Hutton Oddy, H.W. Raadsma, C. Wade, J. Wang, W. Wang and X. Xun. 2010. International Sheep Genomics Consortium: The sheep genome reference sequence: a work in progress. Animal Genetics, 41: 449-453. [DOI:10.1111/j.1365-2052.2010.02100.x]
2. Asadi, A., H. Moradi Shahrbabak, P. Azizi, S. Elahian and S. Abbasi. 2015. Investigation of Polymorphism in Exon 4 of GH Gene and Its Association with Growth Traits in Kermani Sheep using PCR- SSCP. Research on Animal Production, 6(12): 139-144 (In Persian).
3. Barzehkar, R., A. Salehi and F. Mahjoubi. 2009. Polymorphisms of the ovine leptingene and its association with growth and carcass traits in three Iranian sheep breeds. Iranian Journal of Biotechnology, 7: 241-246 (In Persian).
4. Bianchi, P., E. Fermo, C. Vercellati, C. Boschetti, W. Barcellini and A. Iurlo. 2009. Congenital dyserythropoietic anemia type II (CDAII) is caused by mutations in the SEC23B gene. Human Mutation, 30: 1292-1298. [DOI:10.1002/humu.21077]
5. Chen, X.W., H. Wang, K. Bajaj, P. Zhang, Z.M. Meng, D. Ma, Y. Bai, H.H. Liu, E. Adams, A. Baines, G. Yu, M.A. Sartor, B. Zhang, Z. Yi, J. Lin, S.G. Young, R. Schekman and D. Ginsburg. 2013. SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion. elife.elifesciences.org [DOI:10.7554/eLife.00444]
6. Cockett, N.E., M.A. Smith, C.A. Bidwell, K. Segers, T.L. Hadfield, G.D. Snowder, M. Goerges and C. Charlier. 2005. The callipyge mutation and other genes that affect muscle hypertrophy in sheep. Genetic Selection Evolution, 37: S65-S81. [DOI:10.1051/gse:2004032]
7. Freking, B.A., J.W. Keele, S.D. Shackelford, T.L. Wheeler, M. Koohmaraie, M.K. Nielsen and K.A. Leymaster. 1999. Evaluation of the ovine callipyge locus: III. Genotypic effects on meat quality traits. Journal of Animal Science, 77: 2336-2344. [DOI:10.2527/1999.7792336x]
8. GAN, S.Q., Z. Wei, S. Min, L. Huan, Y. Jing-Quan, L. Yao-Wei, G. Lei, L. Shou-Ren and W.
9. Xin-Hua. 2013. Correlation analysis between polymorphism of the 9383635th locus on X chromosome and fat-tail trait in sheep. Hereditas, 10: 1209-1218. [DOI:10.3724/SP.J.1005.2013.01209]
10. Ge, W., M.E. Davis, H.C. Hines, K.M. Irvin and R.C.M. Simmen. 2001. Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. Journal of Animal Science, 79: 1757-1762. [DOI:10.2527/2001.7971757x]
11. Gokdal, O., H. Ulker, F. Karakus, F. Cengiz, C. Temur and H. Handil. 2004. Growth, feedlot performance and carcass characteristics of Karakas and crossbred lambs (F1)(Ile de France x Akkaraman (G1) x Karakas) under rural farm conditions in Turkey. South African Journal of Animal Science, 34: 223-232.
12. Karamichou, E., R. Richardson, G. Nute, K. Gibson and S. Bishop. 2006. Genetic analyses and quantitative trait loci detection, using a partial genome scan, for intramuscular fatty acid composition in Scottish Blackface sheep. Journal of Animal Science, 84: 3228-3238. [DOI:10.2527/jas.2006-204]
13. Meuwissen, T.H.E. and M.E. Goddard. 1996. The use of marker haplotypes in animal breeding schemes. Genetics Selection Evolution, 28: 161-176. [DOI:10.1051/gse:19960203]
14. Moradi, M.H., A. Nejati-Javaremi, M. Moradi-Shahrbabak and J.C. McEwan. 2011. Genomic scan of selective sweeps in thin and fat tail sheep breeds for identifying of candidate regions associated with fat deposition. PhD Thesis, Tehran University, Karadj, Iran. 95 pp (In Persian). [DOI:10.1186/1471-2156-13-10]
15. Moradi, M.H., A. Nejati-Javaremi, M. Moradi-Shahrbabak, K.G. Dodds and J.C. McEwan. 2012. Genomic scan of selective sweeps in thin and fat tail sheep breeds for identifying of candidate regions associated with fat deposition. BMC Genetics, 13: 10-25. [DOI:10.1186/1471-2156-13-10]
16. Negussie, E., O. Rottmann, F. Pirchner and J. Rege. 2003. Patterns of growth and partitioning of fat depots in tropical fat-tailed Menz and Horro sheep breeds. Meat science, 64:491-498. [DOI:10.1016/S0309-1740(02)00227-9]
17. POPGENE. 1999. Version 1.32: the user friendly software for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, AB, Canada.
18. Oligo7. 2009. Version 7. OLIGO Primer Analysis Software. Molecular Biology Insights, Inc., Cascade, CO, USA. http://www.oligo.net/
19. SAS Institute. 2000. Statistical Analysis System User's Guide. Statistics (8th Edition) SAS Institute Inc. North Carolina, USA.
20. Sun, Y., G. Zhao, R. Liu, M. Zheng, Y. Hu, D. Wu, L. Zhang, P. Li and J. Wen. 2013. The identification of 14 new genes for meat quality traits in chicken using a genome-wide association study. BMC Genomics, 14: 458-464. [DOI:10.1186/1471-2164-14-458]
21. Vatankhah, M., M. Moradi-Shahrbabak, A. Nejati-Javaremi, S.R. Miraie Ashtiani and R. Vaez Torshizy. 2006. Investigation on fat-tail dimension and its relationship with Lori-Bakhtiari sheep's tail weight. Journal of Science and Technology of Agriculture and Natural Resources, Tenth year, No. III (B), autumn (In persian).
22. Wang, Q., H. Li, N. Li, L. Leng, Y. Wang and Z. Tang. 2006. Identification of single nucleotide polymorphism of adipocyte fatty Acid-Binding protein gene and its association with fatness traits in the chicken. Poultry Science, 85: 429-434. [DOI:10.1093/ps/85.3.429]
23. Xia, W. and S.F.A. Grant. 2013. The genetics of human obesity. Annals of New York Academy of Science, 1281: 178-190. [DOI:10.1111/nyas.12020]
24. Zerehdaran, S., S. Alijani and M. Salehinasab. 2016. Detecting Major Genes for Some Economic Traits in Native Fowl of Yazd Province using Different Statistical Methods. Research on Animal Production, 7(13): 163-170 (In Persian). [DOI:10.18869/acadpub.rap.7.13.170]
25. Zhu, X., N. Niu, Y. Liu, T. Du, D. Chen and X. Wang. 2006. Improvement of the sensitivity and resolution of PCR-SSCP analysis with optimized primer concentrations in PCR products. Journal of genetics, 85: 233-243. [DOI:10.1007/BF02935339]

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