Volume 8, Issue 17 (1-2018)                   rap 2018, 8(17): 88-92 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

(2018). Association of Polymorphisms of Exon 2 of Transcription Factor 7 Gene with Fat Traits of Blood and Fat Tail in Lori-Bakhtiari and Zel Sheep Breeds. rap. 8(17), 88-92. doi:10.29252/rap.8.17.88
URL: http://rap.sanru.ac.ir/article-1-856-en.html
Abstract:   (3504 Views)
Transcription factor 7 gene (TCF7) is located on genomic candidate region for fat traits on sheep chromosome no. 5  and it is excepted to affect fat traits. In the present study, blood samples were collected from 164 Lori-Bakhtiari sheep in Shouli breeding station  located at Shahr-e-Kord city, and 113 Zel sheep in Shirang breeding station located at Gorgan city. DNA was extracted from blood samples using modified salting out method and polymerase chain reactions were performed for amplification of 643 bp fragment of exon 2 of locous TCF7 gene. Determination of genotype patterns of sheep were obtained according to result of vertical electrophoresis and AA, AB and BB genotypes were revealed. In  Zel breed, AA and AB genotype patterns  were observed with frequency of 71 and 29 percent respectively and in Lori-bakhtiari breed, AB and BB genotype patterns were observed with frequency of 10 and 90 percent. There was no significant effect of  genotype patterns  on fat traits In Zel breed but it was significant effect in Lori-Bakhtiari breed. AB genotype had the greatest impact on fat-tail weight and blood cholesterol and BB genotype had considerable effect on blood triglycerides.
Full-Text [PDF 442 kb]   (887 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/01/10 | Accepted: 2018/01/10 | Published: 2018/01/10

References
1. Bonifácio, C., I. Santos, C. Belo and A. Cravador. 2001. Single-Strand conformation polymorphism (SSCP) analysis of alfas1-casein, beta-casein and k-casein genes in charnequeira portuguese indigenous goat breed. Archivos de zootecnia, 50: 105-111.
2. Gokdal, O., H. Ulker, F. Karakus, F. Cengiz, C. Temur and H. Handil. 2004. Growth, feedlot performance and carcass characteristics of Karakas and crossbred lambs (F1)(Ile de France x Akkaraman (G1) x Karakas) under rural farm conditions in Turkey. South African Journal of Animal Science, 34: 223-232.
3. Karamichou, E., R. Richardson, G. Nute, K. Gibson and S. Bishop. 2006. Genetic analyses and quantitative trait loci detection, using a partial genome scan, for intramuscular fatty acid composition in Scottish Blackface sheep. Journal of animal science, 84: 3228-3238. [DOI:10.2527/jas.2006-204]
4. Kingsmore, S., M. Watson and M. Seldin. 1995. Genetic mapping of the T lymphocyte-specific transcription factor 7 gene on mouse Chromosome 11. Mammalian Genome, 6: 378-382. [DOI:10.1007/BF00364808]
5. Miller, S., D. Dykes and H. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic acids research, 16: 1215-1220. [DOI:10.1093/nar/16.3.1215]
6. Moradi, MH., A. Nejati-Javaremi, M. Moradi-Shahrbabak, KG. Dodds and JC. McEwan. 2012. Genomic scan of selective sweeps in thin and fat tail sheep breeds for identifying of candidate regions associated with fat deposition. BMC genetics, 13: 10-19. [DOI:10.1186/1471-2156-13-10]
7. Negussie, E., O. Rottmann, F. Pirchner and J. Rege. 2003. Patterns of growth and partitioning of fat depots in tropical fat-tailed Menz and Horro sheep breeds. Meat science, 64: 491-498. [DOI:10.1016/S0309-1740(02)00227-9]
8. Orita, M., H. Iwahana, H. Kanazawa, K. Hayashi and T. Sekiya. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proceedings of the National Academy of Sciences, 86: 2766-2770. [DOI:10.1073/pnas.86.8.2766]
9. Roose, J., G. Huls, M. Van Beest, P. Moerer, K. vander Horn and R. Goldschmeding. 1999. Synergy between tumor suppressor APC and the β-catenin-Tcf4 target Tcf1. Science, 285: 1923-1926. [DOI:10.1126/science.285.5435.1923]
10. Untergasser, A., H. Nijveen, X. Rao, T. Bisseling, R. Geurts and J.A. Leunissen. 2007. Primer3Plus, an enhanced web interface to Primer3. Nucleic acids research, 35: 71-74. [DOI:10.1093/nar/gkm306]
11. van de Wetering, M., M. Oosterwegel, D. Dooijes and H. Clevers. 1991. Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box. The EMBO journal, 10: 123-127. [DOI:10.1002/j.1460-2075.1991.tb07928.x]
12. Vatankhah, M., M. Moradi-Shahebabak, A. Nejati-Javaremi, R. Miraei-Ashtiani and R. Vaez Torshizi. 2005. The relationships between body and fat-tail measurements with weights of live, hot carcass and hot carcass without fat-tail in lori-bakhtiari sheep. Pajouhesh and sazandeghi, 64:66-74.
13. Zhu, X., N. Niu, Y. Liu, T. Du, D. Chen and X. Wang. 2006. Improvement of the sensitivity and resolution of PCR-SSCP analysis with optimized primer concentrations in PCR products. Journal of genetics, 85: 233-238. [DOI:10.1007/BF02935339]

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Research On Animal Production

Designed & Developed by : Yektaweb