Volume 9, Issue 19 (6-2018)
rap 2018, 9(19): 26-31 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shawrang P, Mortaheb M, Motamedi-sede F, Askari H. (2018). Application of Gamma Irradiation for Eliminating Bacterial Contamination of Bovine Colostrums. rap. 9(19), 26-31. doi:10.29252/rap.9.19.26
URL: http://rap.sanru.ac.ir/article-1-723-en.html
URL: http://rap.sanru.ac.ir/article-1-723-en.html
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran
Abstract: (3697 Views)
In order to colostrum pasteurization and creating of dose-response curve for Escherichia coli and Staphylococcus aurous, the colostrum of cow was gamma irradiated at doses of 0, 0.5, 1 and 2 KGy. Chemical composition, true protein, electrophoretic pattern of proteins, lactose content, peroxide level and pH of colostrums samples before and after of irradiation were determinate. The obtained data were analysed using proc GLM of SAS software appropriate for completely randomized design. The effect of irradiation was significant on Escherichia coli and Staphylococcus aurous contamination of colostrums (p<0.05). The Escherichia coli and Staphylococcus aurous contamination decreased as irradiation dose increased and reach to zero at dose of 2 kGy. Gamma irradiation had no effect (p>0.05) on chemical composition, true protein, fatty acid composition, peroxide level, pH and electrophoretic pattern of proteins and lactose content. Based on the results of this study, the appropriate dose for decrease in log cycle of Escherichia coli and Staphylococcus aurous population in colostrum is 0.68 and 1.15 kGy respectively. Therefore, gamma irradiation at low doses can eliminate Escherichia coli and Staphylococcus aurous contamination without negative impact on colostrum quality.
Keywords: Colostrum, Chemical composition, Escherichia coli, Gamma irradiation, Staphylococcus aurous
Type of Study: Research |
Subject:
تغذیه نشخوارکنندگان
Received: 2017/02/8 | Revised: 2018/06/24 | Accepted: 2017/11/6 | Published: 2018/06/24
Received: 2017/02/8 | Revised: 2018/06/24 | Accepted: 2017/11/6 | Published: 2018/06/24
References
1. Amini-robati, E., P. Shawrang, F. Fattahnia and A. Mehrabi. 2013. The effect of irradiation on immunoglobulin G concentration in colostrums. Proceeding of National Congress on Animal and Poultry in North of Iran. Sari Agricultural Science and Natural Resources University, pp: 1614-1617 (In Persian).
2. Amini-robati, E., P. Shawrang, F. Fattahnia and A. Mehrabi. 2014. The comparison between irradiation and freezing effects on colostrum quality. The 6th Iranian Congress on Animal Science. The University of Tabriz, 113 pp (In Persian).
3. Association of Official Analytical Chemists. 2000. Official Methods of Analysis of the Association of Official Analytical Chemists, 17th edition Arlington, VA.
4. Bradford, M.M. 1997. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248. [DOI:10.1016/0003-2697(76)90527-3]
5. Campbell, J.M., L.E. Russell, J.D. Crenshaw, E.M. Weaver, S. Godden, J.D. Quigley, J. Coverdale and H. Tyler. 2007. Impact of irradiation and immunoglobulin G concentration on absorption of protein and immunoglobulin G in calves fed colostrum replacer. Journal of Dairy Science, 90: 5726-5731. [DOI:10.3168/jds.2007-0151]
6. Esterbauer, H. and K.H. Cheeseman. 1990. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in Enzymology, 186: 407-421. [DOI:10.1016/0076-6879(90)86134-H]
7. Food and Agriculture Organization. 2003. General Standard for Irradiated Foods, CODEX STAN106-1983, Rev.1-2003, FAO/WHO, Rome.
8. Garbarino, C., G. Cammi, I. Archetti, M. Amadori, G. Panella, M. Ricchi, V. Tranquillo and N. Arrigoni. 2012. Heat treatment and gamma-irradiation of bovine colostrum: Impact on survival of Mycobacterium avium subsp. paratuberculosis (MAP) and other pathogens and on immunoglobulin content Proceedings of the 11th International Colloquium on Paratuberculosis. Sydney, Australia, 197.
9. Garin-Bastuji, B., B. Perrin, M.F. Thorel and J.L. Martel. 1990. Evaluation of γ-ray irradiation of cows' colostrum for Brucella abortus, Escherichia coli K99, Salmonella dublin and Mycobacterium paratuberculosis decontamination. Letters in Applied Microbiology, 11: 163-16. [DOI:10.1111/j.1472-765X.1990.tb00150.x]
10. Green, L., S. Godden and J. Feirtag. 2003. Effect of batch and high temperature-short time pasteurization on immunoglobulin G concentrations in colostrum. Journal of Dairy Science, 86 246 pp.
11. Godden, S.M., S. Smith, J.M. Feirtag, L.R. Green, S.J. Wells and J.P. Fetrow. 2003. Effect of On-Farm Commercial Batch Pasteurization of Colostrum on Colostrum and Serum Immunoglobulin Concentrations in Dairy Calves. Journal of Dairy Science, 86: 1503-1512. [DOI:10.3168/jds.S0022-0302(03)73736-9]
12. Godden, S., S. McMartin, J. Feirtag, J. Stabel, R. Bey, S. Goyal, L. Metzger, J. Fetrow, S. Wells and H. Chester-Jones. 2006. Heat-Treatment of Bovine Colostrum. II: Effects of Heating Duration on Pathogen Viability and Immunoglobulin G. Journal of Dairy Science, 89: 3476-3483. [DOI:10.3168/jds.S0022-0302(06)72386-4]
13. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680 pp. [DOI:10.1038/227680a0]
14. McGuirk, S. and M. Collins. 2004. Managing the production, storage and delivery of colostrum. in Veterinary Clinics of North America Food Animal Practice. W.B. Saunders, New York, NY, 593-603. [DOI:10.1016/j.cvfa.2004.06.005]
15. McMartin, S., S. Godden, L. Metzger, J. Feirtag, R. Bey, J. Stabel, S. Goyal, J. Fetrow, S. Wells and H. Chester-Jones. 2006. Heattreatment of bovine colostrums. I: Effects of temperature on viscosity and immunoglobulin G level. Journal of Dairy Science, 89: 2110-2118. [DOI:10.3168/jds.S0022-0302(06)72281-0]
16. Negrulescu, A., V. Patrulea, M.M. Mincea, C. Ionascu, B.A. Vlad-Oros and V. Ostafe. 2012. Adapting the reducing sugars method with dinitrosalicylic acid to microtiter plates and microwave heating. Journal of the Brazilian Chemical Society, 23: 2176-2182. [DOI:10.1590/S0103-50532013005000003]
17. Poulsen, K.P., F.A. Hartmann and S.M. McGuirk. 2002. Bacteria in colostrum: impact on calf health, in Proc. 20th American College of Internal Veterinary Medicine: Mira Digital Publishing. 773 pp.
18. Shannon, L.H., C. Vargas-Aburto, M.U. Roberto and C.J. Woolverton. 2005. Inactivation of Bacillus Endospores in envelopes by electron beam irradiation. Applied and Environmental Microbiology, 11-71. [DOI:10.1128/AEM.71.11.7029-7032.2005]
19. Trampuz, A., K.E. Piper, J.M. Steckelberg and R. Patel. 2006. Effect of gamma irradiation on viability and DNA of Staphylococcus epidermidis and Escherichia coli. Journal of Medical Microbiology, 55: 1271-1275. [DOI:10.1099/jmm.0.46488-0]
20. Tran, H., K. Marlowe, K. McKenney, G. Petrosian, Y. Griko, W.H. Burgess, W.N. Drohan, MA. Imboden, C. Kempf, N. Boschetti and D.M. Mann. 2004. Functional integrity of intravenous immunoglobulin following irradiation with a virucidal dose of gamma radiation. Biologicals, 32: 94-104. [DOI:10.1016/j.biologicals.2004.01.002]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
