Research On Animal Production

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Veshkini A, Kouhkan F, Assadi Alamouti A, Salehi A, Mohammadi-sangcheshmeh A. (2021). The Inhibitory Effect of Fish Oil on Mesenchymal Stem Cells Differentiation to Adipocytes in Sheep. rap. 12(32), 88-95. doi:10.52547/rap.12.32.88
URL: http://rap.sanru.ac.ir/article-1-1139-en.html

    In this study, the expression of genes and miRNAs related to differentiation, cell cycle and metabolism was investigated in Zandi sheep mesenchymal cells (ADSC) under fish oil treatment as a source of omega-3 fatty acids. Isolated sheep mesenchymal stem cells were confirmed for pluripotency. ADSCs were treated in the presence or absence of 15 mg / ml fish oil, and cell cycle and apoptosis induction were measured in each group (three independent repeat) via Flow cytometry. In a similar experiment, the expression of downstream miRNAs and genes at the growth and differentiation stages to adipocyte was compared in both groups. Mesenchymal cells treated with fish oil had a higher percentage of late apoptosis, while the percentage of cells in S phase decreased. The expression of miRNAs did not change significantly, however, let-7a was significantly decreased in the fish oil group. Fish oil treatment decreased (0.39) relative expression of peroxisome proliferator-activated receptor G (PPARG) and increased (1.85) Interleukin 1 beta expression. In adipocyte-differentiated cells, PPARG expression was decreased. The results of this study showed the inhibitory properties of fish oil on adipocyte differentiation of mesenchymal cells through direct or indirect effects on PPAR pathway. However, no significant difference was observed in the expression of selected miRNAs.