Research on Animal Production
پژوهشهاي توليدات دامي
Res Anim Prod
Agriculture
http://rap.sanru.ac.ir
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admin
2251-8622
2676-461X
8
10.61882/rap
14
8888
13
fa
jalali
1405
7
1
gregorian
2026
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3
online
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fulltext
fa
مطالعه چند شکلی اگزون 2 ژن گیرنده هورمون گرلین (GHSR) و ارتباط آن با صفات رشد و ابعاد بدن در گوسفند نژاد لری بختیاری
Study of polymorphism of Exon 2 on growth hormone secretagogue receptor gene (GHSR) and its association with growth and body dimensions traits in Lori-Bakhtiari breed sheep
ژنتیک و اصلاح نژاد دام
ژنتیک و اصلاح نژاد دام
پژوهشي
Research
<p><span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">مقدمه وهدف:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">یکی از اهداف تشخیص جایگاه­های ژنی مرتبط با صفات کمی، یافتن جایگاه ژن­های مؤثر بر صفات اقتصادی مرتبط با رشد است، در این حالت شناسایی ژن­های با اثرات عمده بر روی این صفات اهمیت خاصی در پیشرفت برنامه­های اصلاح نژادی می­تواند داشته باشد. هدف ازاین پژوهش ارتباط ژن گیرنده هورمون گرلین </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">(GHSR)</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> با صفات ریخت شناسی و صفات رشد در گوسفندان نژاد لری بختیاری با استفاده از روش</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">PCR-SSCP </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">و توالی­یابی محصولات </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">PCR</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> بود.</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">مواد و روش­ها:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">به این منظور، از</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">سیاهرگ</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">وداج تعداد</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">147</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">رأس</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">گوسفند</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">نژاد لری بختیاری</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">خونگیری</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">شد. استخراج </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">DNA</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">به روش فنول کلروفرم انجام گرفت، تکثیر قطعه 452 جفت بازی از اگزون 2 ژن </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">GHSR</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> با استفاده از یک جفت آغازگر اختصاصی طراحی شده انجام گرفت. برای مشاهده چند شکلی فضایی تک رشته</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">­</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">ای</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black"> (SSCP) </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">محصولات</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black"> PCR </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">از ژل اکریل آمید 12% و رنگ آمیزی نیترات نقره استفاده گردید. </span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">یافته­ها:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">پس از توالی­یابی، یک چندشکلی تک نوکلئوتیدی در این ژن در موقعیت نوکلئوتیدی باز 155 تبدیل</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">T </span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">به </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">C</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> برای اولین بار شناسایی شد. اثر این </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">SNP</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">بر صفات وزن تولد و عرض بالای دنبه در نژاد لری بختیاری، معنی دار( 05/0></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">P</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">) بود و ژنوتیپ </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">CC</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> اثر بیشتری نسبت به ژنوتیپ </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:115%"><span style="color:black">CT</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black"> داشت.</span></span></span></span> <span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">نتیجه گیری:</span></span></span></span></b> <span lang="FA" style="font-size:10.0pt"><span style="line-height:115%"><span b="" mitra="" style="font-family:"><span style="color:black">نتایج این پژوهش نشان می­دهد که ژن گیرنده هورمون گرلین می­تواند کاندید مناسبی برای انتخاب برای صفت وزن تولد در نژاد لری بختیاری به عنوان یک نشانگر ژنتیکی باشد.</span></span></span></span><span lang="AR-SA" style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span></span><br>
</p>
<p><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Introduction and purpose:</span></span></b><span style="font-size:11.0pt"><span style="line-height:115%"> One of the goals of identifying gene locations related to quantitative traits is to find the location of genes that affect economic traits related to growth, in this case, identifying genes with major effects on these traits can be of particular importance. in the progress of the eugenics program.The association of Ghrelin receptor gene (GHSR) with morphological and growth traits in Lori-Bakhtiari sheep breed were studied using PCR-SSCP and DNA sequencing.</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Material and Methods:</span></span></b><span style="font-size:11.0pt"><span style="line-height:115%"> Blood samples were collected from jugular vein of 147 Lori-Bakhtiari sheep. Genomic DNA was extracted using phenols chloroform method. The exon 2 (452 bp) of GHSR gene was amplified using designed specific primers. The single stranded conformation polymorphism (SSCP) was used to identify polymorphism on the patterns of PCR product using 12% Acrylamide gel and silver staining method.</span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Results:</span></span></b><span style="font-size:11.0pt"><span style="line-height:115%"> After sequencing, one novel single nucleotide polymorphisms (silent substitution) identified in position 155 which changed nucleotide T to C. The association of identified SNP with birth weight and the upper wide fat-tail in Lori-Bakhtiari, was significant (p<0.05) and the CC genotype was higher effect than CT genotype. The studied traits CC genotype was more desirable than CT genotype.</span></span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:115%">Conclusion:</span></span></b><span style="font-size:11.0pt"><span style="line-height:115%"> The results showed that the gherlin receptor gene (GHSR) could be a possible candidate in birth weight trait for using selection in Lori-Bakhtiari sheep as a genetic marker.</span></span></span></span></span></span><br>
</p>
چندشکلی, صفات رشد, گوسفند لری بختیاری, .GHSR
Polymorphism, GHSR gene, growth traits, Lori-Bakhtiari sheep.
0
0
http://rap.sanru.ac.ir/browse.php?a_code=A-10-185-5&slc_lang=fa&sid=1
Abbas
Abbasi-Firoozjaei
مهدی
عباسی فیروزجایی
mahdiabbasi644@yahoo.com
100319475328460027242
100319475328460027242
No
University of Tehran
دانشگاه تهران
Hossein
Moradi Shahrbabak
حسین
مرادی شهربابک
hmoradis@ut.ac.ir
100319475328460027243
100319475328460027243
Yes
University of Tehran
دانشگاه تهران
Hassan
Mehrbani Yeganeh
حسن
مهربانی یگانه
hmehrbani@ut.ac.ir
100319475328460027244
100319475328460027244
No
University of Tehran
دانشگاه تهران
Milad
Gholami Gholamitahoone
میلاد
غلامی طاحونه
gholami.milad@ut.ac.ir
100319475328460027245
100319475328460027245
No
University of Tehran
دانشگاه تهران