fa تاثیر اسید رتینوئیک بر بلوغ برون تنی تخمک های نابالغ کومولوس دار گوسفند تخصصي Special پژوهشي Research <p align="right" style="DIRECTION: rtl"><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span style="mso-spacerun: yes"><font face="Times New Roman" size="2"><strong>&nbsp;&nbsp;&nbsp;&nbsp; </strong></font></span></span><strong><font face="Times New Roman"><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">هدف از این تحقیق بررسی تاثیر دوزهای مختلف اسید رتینوئیک ال ترانس بر بلوغ برون تنی<br> تخمک های کومولوس دار نابالغ گوسفند بود. تخمک های کومولوس دار از فولیکول هایی با قطر 6-2<br> میلی متر به روش آسپیره</span><span dir="ltr"></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" lang="FA" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="ltr"></span> </span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">کردن گرفته شدند. اسید رتینوئیک ال ترانس در دوزهای 0، 1، 5/1 و 2 میکرومولار به محیط کشت بلوغ اضافه شدند. برای انجام فرآیند بلوغ همه تیمارها به مدت 24 ساعت داخل انکوباتور </span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">C</span><span dir="rtl"></span>^{<span 10.0pt="" 12pt="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span>&deg;</span>}<span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">5/38 قرار داده شدند. فرآیند آغاز میوز و بلوغ تخمک پس از رنگ آمیزی با میکروسکوپ اینورت بررسی و نتایج حاصل با رویه </span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">GLM</span><span dir="rtl"></span></font></strong><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><strong><font face="Times New Roman"> تجزیه و تحلیل شدند. هیچ یک از<br> تخمک ها در گامه </font></strong></span><strong><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">GV</span><span dir="rtl"></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><font face="Times New Roman"> باقی نماندند. تعداد تخمک ها در گامه </font></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">GVBD</span><span dir="rtl"></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><font face="Times New Roman"> در تیمار کنترل نسبت به تیمار دو میکرومولار افزایش معنی داری را نشان داد</font></span><span dir="ltr"></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" lang="FA" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="ltr"></span> </span><span dir="rtl"></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><font face="Times New Roman">(05/0></font></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">P</span><span dir="rtl"></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span><font face="Times New Roman">).</font></span><span dir="ltr"></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" lang="FA" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="ltr"></span> </span><font face="Times New Roman"><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">نرخ </span><span dir="ltr"></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" lang="FA" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="ltr"></span><span style="mso-spacerun: yes"><font size="2">&nbsp;</font></span></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">بلوغ تخمک در غلظت 2 میکرومولار نیز نسبت به تیمار کنترل و تیمار یک میکرومولار افزایش معنی داری را نشان داد</span><span dir="ltr"></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" lang="FA" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="ltr"></span> </span><span dir="rtl"></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span>(05/0></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">P</span><span dir="rtl"></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span>). نتایج حاصل از این بررسی نشان داد، افزودن دو میکرومولار اسید رتینوئیک به محیط کشت بلوغ حاوی تخمک های نابالغ، بلوغ تخمک ها را در محیط کشت</span><span dir="ltr"></span><span 10pt="" 12.0pt="" 2="" calibri="" en-us="" fa="" font-size:="" lang="FA" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="ltr"></span> </span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">تجاری </span><span 10pt="" 12.0pt="" 2="" ar-sa="" calibri="" en-us="" font-size:="" mso-ansi-language:="" mso-bidi-font-family:="" mso-bidi-font-size:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times="">TCM199</span><span dir="rtl"></span><span 10.0pt="" 12pt="" 2="" calibri="" dir="rtl" en-us="" fa="" font-size:="" lang="FA" mso-ansi-font-size:="" mso-ansi-language:="" mso-ascii-font-family:="" mso-bidi-language:="" mso-fareast-font-family:="" mso-fareast-language:="" mso-hansi-font-family:="" new="" style="LINE-HEIGHT: 115% FONT-FAMILY: " times=""><span dir="rtl"></span> بهبود می بخشد.</span></font></strong></p> <br> &nbsp;&nbsp;&nbsp;&nbsp; This study was carried out to investigate the effect of all-trans retinoic acid (t-RA) on in vitro maturation of ovine immature oocytes containing cumulus cells. The Oocytes with cumulus cells were recovered from follicles (2-6 mm in diameter). Different concentrations of t-RA (0, 1, 1.5 and 2 &mu;mol) were added in the maturation medium. For oocytes maturation, all groups were located in the CO2 incubator for 24 hours. The meiosis and nuclear maturation status of the oocytes in each experimental group were assessed using an invert microscope and the data were analyzed with GLM procedure. There are not any oocytes in GV stage. The percentage of oocytes in GVBD stage at control group in comparison with 1 &mu;mol and 2 &mu;mol treatments was significant (p<0.05). The rate of oocytes maturation in 2 &mu;mol retinoic acid treatments were significantly more than other groups (P<0.05). In conclusion, the use of 2 &mu;mol retinoic acid increased the ovine oocyte maturation significantly in the commercial TCM199 medium.<span dir="RTL"></span><br> &nbsp; اسید رتینوئیک, بلوغ برون تنی, GV, GVBD, تخمک نابالغ In vitro maturation, Retinoic acid, GV, GVBD, Ovine oocyte 49 58 http://rap.sanru.ac.ir/browse.php?a_code=A-10-1-28&slc_lang=fa&sid=1 Marzieh Khani مرضیه خانی 10031947532846009894 10031947532846009894 Yes Collage of Animal Science, University of Tabriz گروه علوم دامی، دانشگاه تبریز Hossein Daghigh kia حسین دقیق‌کیا 10031947532846009895 10031947532846009895 No Collage of Animal Science, University of Tabriz گروه علوم دامی دانشگاه تبریز Ahmad Zare-Shahneh احمد زارع شحنه 10031947532846009896 10031947532846009896 No Collage of Animal Science, University of Tehran, Karaj گروه علوم دامی دانشگاه تهران Sadegh Alijani صادق علیجانی 10031947532846009897 10031947532846009897 No Collage of Animal Science, University of Tabriz گروه علوم دامی دانشگاه تبریز