Volume 10, Issue 24 (9-2019)                   rap 2019, 10(24): 76-84 | Back to browse issues page


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Deldar H. The use of Royal Jelly as a Replacement of Fetal Bovine Serum in In Vitro Production of Goat Embryo with Emphasis on Apoptosis Related Genes. rap. 2019; 10 (24) :76-84
URL: http://rap.sanru.ac.ir/article-1-950-en.html
Sari Agricultural Sciences and Natural Resources University
Abstract:   (849 Views)
The present study was conducted to investigate the effect of different concentrations of royal jelly as a fetal bovine serum replacement on in vitro embryo production of goat oocytes and the gene expression involved in apoptosis. In vitro maturation (IVM) of oocyte was performed in the presence of control (10% FBS), 10 mg/ml RJ (without FBS), 5% FBS and 5 mg/ml RJ, 2.5% FBS and 7.5 mg/ml RJ, 7.5% FBS and 2.5 mg/ml RJ. Nuclear statusof matured oocyte and mRNA abundance of selected genes were evaluated following 24 h of IVM. Following the IVM, fertilization and embryo culture were carried out in all groups and embryonic development was examined. Our data suggested that the number of oocytes at metaphase II stage, cleavage and blastocyst stage of the embryo were gradually increased followed by the dose of royal jelly gradually increased in the maturation medium. The addition of 10 mg/ml royal jelly to the maturation media was significantly increased (P <0.05) maturation rate (91.35%) of goat oocyte, cleavage (83.39%) and blastocyst formation (30.18%) compared with the control groups (71.31%, 62.50% and 21.42%, respectively). By increasing of royal jelly concentrations, the mRNA transcript of the BCL2 gene was increased, while transcript abundance of BAX was significantly decreased. BCL2/BAX ratio has also been significantly increased (P <0.05) by increasing of royal jelly concentrations in the maturation media. However, relative gene expression of CASPAS3 gene was not significantly different between treatments. It seems that the gradual increase of royal jelly as a replacement of FBS in the maturation media had a desirable effect on oocyte maturation and the embryo development condition of caprine oocyte.
Full-Text [PDF 560 kb]   (163 Downloads)    
Type of Study: Research | Subject: فیزیولوژی
Received: 2018/08/15 | Revised: 2019/09/18 | Accepted: 2018/10/21 | Published: 2019/09/18

References
1. Eshtiyaghi, M., H. Deldar, Z. Ansari Pisaraie and B. Shohreh. 2014. Effect of royal jelly on glucose metabolism in in vitro maturation and fertilization of sheep oocyte: glycolysis and penthose phosphate pathway. 6th Iranian Animal Science Congress, Tabriz, Iran, 1-4.
2. Mohamadi, S., H. Deldar, Z. Ansari Pisaraie and B. Shohreh. 2016. Effect of royal jelly on genes encoding antioxidant enzymes in in vitro embryo production of goat. Animal Production, 18: 867-876.
3. Valioallahpor Amiri, M., H. Deldar and Z. Ansari pisaraie. 2012. Role pf royal jelly on in vitro maturation of sheep oocyte. International congress on reservation of genetic resources of Zel and Dalagh sheep. Gonbad, Iran, 527-531.
4. Bavister, B.D. 1995. Culture of preimplantation embryos: facts and artifacts. Human Reproduction Update, 1: 91-148. [DOI:10.1093/humupd/1.2.91]
5. Chen, D., X.X. Xin, H.C. Qian, Z.Y. Yu and L.R. Shen. 2016. Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 17(6): 476-483. [DOI:10.1631/jzus.B1500295]
6. Fakhr, M., M. Mirzaei, A. Rafei, S. Armat and M. Mojtahedian. 2012. Comparative evaluation of hydatid cyst fluid and fetal bovine serum (fbs) in culture medium of rat fibroblast cells. Journal of Mazandaran University of Medical Sciences, 22: 222-230.
7. Gaddiner, C. and D.J. Reed. 1994. Status of glutathione during oxidant-induced oxidative stress in the pre-implantation mouse embryo. Biology of Reproduction, 51: 1307-1314. [DOI:10.1095/biolreprod51.6.1307]
8. Gardner, D.K. 1994. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biology International, 18: 1163-1179. [DOI:10.1006/cbir.1994.1043]
9. Gardner, D.K. 1999. Development of serum-free culture systems for the ruminant embryo and subsequent assessment of embryo viability.In: Proceedings 5th International Symposium on Reproduction in Domestic Ruminants, pp: 461-475.
10. Gardner, D.K. and M. Lane. 1997. Culture and selection of viable blastocysts: a feasible proposition for human IVF. Human Reproduction Update, 3: 367-382. [DOI:10.1093/humupd/3.4.367]
11. Gstraunthaler, G. 2003. Alternatives to the use of fetal bovine serum: Serum-free cell culture. Altex., 20: 275-281.
12. Guo, H., Y. Kouzuma and M. Yonekura, 2008a. Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry, 113: 238-245. [DOI:10.1016/j.foodchem.2008.06.081]
13. Inoue, T. 1986. The use and utilization of royal jelly and the evaluation of the medical efficacy of royal jelly in Japan.Proc. XXXthInternat.Congr.Apicult., Nagoya, Apimondia, 444-447.
14. Marghitaş, L.A. 2008. Produseleapicolesiprincipalelelorinsuşiriterapeutice In: Albinelesiproduselelor. Ceres, Bucharest, pp: 280-378.
15. Mazangi, H.R., H. Deldar, N.E. Kashan and A. Mohammadi-Sangcheshmeh. 2014. Royal jelly treatment during oocyte maturation improves in vitro meiotic competence of goat oocytes by influencing intracellular glutathione synthesis and apoptosis gene expression. Reproduction, Fertility and Development, 27: 241. [DOI:10.1071/RDv27n1Ab305]
16. Nagai, T. and R. Inoue. 2004. Preparation and the functional properties of water and alkaline extract of royal jelly. Food Chemistry, 84: 181-186. [DOI:10.1016/S0308-8146(03)00198-5]
17. Nagai, T.R., R. Inoue, N. Suzuki and T. Nagashima. 2006. Antioxidant properties of enzymatic hydrolysates from royal jelly. Journal of Medicinal Food, 9: 363-367. [DOI:10.1089/jmf.2006.9.363]
18. Nakahara, K., H. Saito, T. Saito, M. Ito, N. Ohta, T. Takahashi and M. Hiroi. 1997. The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs. Fertility and Sterility, 68: 312-7. [DOI:10.1016/S0015-0282(97)81521-X]
19. Onal, A.G., M. Kuran, I. Tapki, E. Sirin and O. Gorgulu. 2005. Honey bee royal jelly: an alternative source to serum for in vitro maturation of ovine oocytes. European Association for Animal Production-56th Annual Meeting, Uppsala, 283.
20. Schmitzová, J., J. Klaudiny, S. Albert, W. Schröder, W. Schreckengost, J. Hanes, J. Júdová and J. Simúth. 1998. A family of major royal jelly proteins of the honeybee Apis mellifera L. Cellular and Molecular Life Sciences, 54(9): 1020-1030. [DOI:10.1007/s000180050229]
21. Valiollahpoor Amiri, M., H. Deldar and Z. Asari Pirsaraei. 2015. Impact of supplementary royl jelly on in vitro maturation of sheep oocytes: genes involved in apoptosis and embryonic development. System Biology in Reproductive Medicine, 62: 31-38. [DOI:10.3109/19396368.2015.1088102]
22. Zhang, G.M., C.H. Gu, Y.L. Zhan, H.Y. Sun, W.P. Qian and Z.R. Zhou. 2013. Age-associated changes in gene expression of goat oocytes. Theriogenology, 80:328-336. [DOI:10.1016/j.theriogenology.2013.04.019]
23. Eshtiaghi M., H. Deldar, Z. Ansari Pirsaraei and B. Shohre. 2016. Royal jelly may improve the metabolism of glucose and redox state of ovine oocytes matured in vitro and embryonic development following in vitro fertilization, Theriogenology, 86: 2210-2221. [DOI:10.1016/j.theriogenology.2016.07.019]
24. Eshtiyaghi, M., H. Deldar, Z. Ansari Pisaraie and B. Shohreh. 2014. Effect of royal jelly on glucose metabolism in in vitro maturation and fertilization of sheep oocyte: glycolysis and penthose phosphate pathway. 6th Iranian Animal Science Congress, Tabriz, Iran, 1-4.
25. Mohamadi, S., H. Deldar, Z. Ansari Pisaraie and B. Shohreh. 2016. Effect of royal jelly on genes encoding antioxidant enzymes in in vitro embryo production of goat. Animal Production, 18: 867-876.
26. Valioallahpor Amiri, M., H. Deldar and Z. Ansari pisaraie. 2012. Role pf royal jelly on in vitro maturation of sheep oocyte. International congress on reservation of genetic resources of Zel and Dalagh sheep. Gonbad, Iran, 527-531.
27. Bavister, B.D. 1995. Culture of preimplantation embryos: facts and artifacts. Human Reproduction Update, 1: 91-148. [DOI:10.1093/humupd/1.2.91]
28. Chen, D., X.X. Xin, H.C. Qian, Z.Y. Yu and L.R. Shen. 2016. Evaluation of the major royal jelly proteins as an alternative to fetal bovine serum in culturing human cell lines. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 17(6): 476-483. [DOI:10.1631/jzus.B1500295]
29. Fakhr, M., M. Mirzaei, A. Rafei, S. Armat and M. Mojtahedian. 2012. Comparative evaluation of hydatid cyst fluid and fetal bovine serum (fbs) in culture medium of rat fibroblast cells. Journal of Mazandaran University of Medical Sciences, 22: 222-230.
30. Gaddiner, C. and D.J. Reed. 1994. Status of glutathione during oxidant-induced oxidative stress in the pre-implantation mouse embryo. Biology of Reproduction, 51: 1307-1314. [DOI:10.1095/biolreprod51.6.1307]
31. Gardner, D.K. 1994. Mammalian embryo culture in the absence of serum or somatic cell support. Cell Biology International, 18: 1163-1179. [DOI:10.1006/cbir.1994.1043]
32. Gardner, D.K. 1999. Development of serum-free culture systems for the ruminant embryo and subsequent assessment of embryo viability.In: Proceedings 5th International Symposium on Reproduction in Domestic Ruminants, pp: 461-475.
33. Gardner, D.K. and M. Lane. 1997. Culture and selection of viable blastocysts: a feasible proposition for human IVF. Human Reproduction Update, 3: 367-382. [DOI:10.1093/humupd/3.4.367]
34. Gstraunthaler, G. 2003. Alternatives to the use of fetal bovine serum: Serum-free cell culture. Altex., 20: 275-281.
35. Guo, H., Y. Kouzuma and M. Yonekura, 2008a. Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry, 113: 238-245. [DOI:10.1016/j.foodchem.2008.06.081]
36. Inoue, T. 1986. The use and utilization of royal jelly and the evaluation of the medical efficacy of royal jelly in Japan.Proc. XXXthInternat.Congr.Apicult., Nagoya, Apimondia, 444-447.
37. Marghitaş, L.A. 2008. Produseleapicolesiprincipalelelorinsuşiriterapeutice In: Albinelesiproduselelor. Ceres, Bucharest, pp: 280-378.
38. Mazangi, H.R., H. Deldar, N.E. Kashan and A. Mohammadi-Sangcheshmeh. 2014. Royal jelly treatment during oocyte maturation improves in vitro meiotic competence of goat oocytes by influencing intracellular glutathione synthesis and apoptosis gene expression. Reproduction, Fertility and Development, 27: 241. [DOI:10.1071/RDv27n1Ab305]
39. Nagai, T. and R. Inoue. 2004. Preparation and the functional properties of water and alkaline extract of royal jelly. Food Chemistry, 84: 181-186. [DOI:10.1016/S0308-8146(03)00198-5]
40. Nagai, T.R., R. Inoue, N. Suzuki and T. Nagashima. 2006. Antioxidant properties of enzymatic hydrolysates from royal jelly. Journal of Medicinal Food, 9: 363-367. [DOI:10.1089/jmf.2006.9.363]
41. Nakahara, K., H. Saito, T. Saito, M. Ito, N. Ohta, T. Takahashi and M. Hiroi. 1997. The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs. Fertility and Sterility, 68: 312-7. [DOI:10.1016/S0015-0282(97)81521-X]
42. Onal, A.G., M. Kuran, I. Tapki, E. Sirin and O. Gorgulu. 2005. Honey bee royal jelly: an alternative source to serum for in vitro maturation of ovine oocytes. European Association for Animal Production-56th Annual Meeting, Uppsala, 283.
43. Schmitzová, J., J. Klaudiny, S. Albert, W. Schröder, W. Schreckengost, J. Hanes, J. Júdová and J. Simúth. 1998. A family of major royal jelly proteins of the honeybee Apis mellifera L. Cellular and Molecular Life Sciences, 54(9): 1020-1030. [DOI:10.1007/s000180050229]
44. Valiollahpoor Amiri, M., H. Deldar and Z. Asari Pirsaraei. 2015. Impact of supplementary royl jelly on in vitro maturation of sheep oocytes: genes involved in apoptosis and embryonic development. System Biology in Reproductive Medicine, 62: 31-38. [DOI:10.3109/19396368.2015.1088102]
45. Zhang, G.M., C.H. Gu, Y.L. Zhan, H.Y. Sun, W.P. Qian and Z.R. Zhou. 2013. Age-associated changes in gene expression of goat oocytes. Theriogenology, 80:328-336. [DOI:10.1016/j.theriogenology.2013.04.019]
46. Eshtiaghi M., H. Deldar, Z. Ansari Pirsaraei and B. Shohre. 2016. Royal jelly may improve the metabolism of glucose and redox state of ovine oocytes matured in vitro and embryonic development following in vitro fertilization, Theriogenology, 86: 2210-2221. [DOI:10.1016/j.theriogenology.2016.07.019]

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