دوره 8، شماره 18 - ( زمستان 1396 )                   جلد 8 شماره 18 صفحات 155-160 | برگشت به فهرست نسخه ها

XML English Abstract Print


چکیده:   (817 مشاهده)
   پرولاکتین از هیپوفیز قدامی ترشح می شود و نقش­های مهمی از جمله در توسعه پستان، تمایز و شیرواری دارد. هدف از این مطالعه ارزیابی چند شکلی ژن گیرنده پرولاکتین در نژاد شال و آمیخته­های شال و رومانوف بود. برای این منظور پس از خونگیری از دام­ها و استخراج DNA به روش فنول کلروفرم، دو قطعه 176 و 215 جفت بازی از اینترون 1 ژن گیرنده پرولاکتین توسط آغازگرهای اختصاصی و طی واکنش PCR تکثیر شدند. سپس محصولات PCR با استفاده از الکتروفورز ژل آکریل آمید و رنگ آمیزی نقره مورد آنالیز قرار گرفتند. نتایج نشان داد در قطعه 176 جفت بازی از ژن گیرنده پرولاکتین (1PRLR) در نژاد شال دو ژنوتیپ AB  و AC مشاهده شد. فراوانی این ژنوتیپ­ها به ترتیب 92/0 و 08/0 و فراوانی آلل­های A، B و C به ترتیب 50/0،46/0 و 04/0 بود. همچنین در جایگاه 1PRLR از این ژن در نژاد آمیخته شال و رومانف فراوانی ژنوتیپ AB و AC به ترتیب 94/0 و 06/0 مشاهده شد و فراوانی آلل­های A، B و C نیز به ترتیب 50/0، 47/0 و 03/0 بود. بررسی چند شکلی در قطعه 215 جفت بازی از ژن گیرنده پرولاکتین (2PRLR) در نژاد شال و آمیخته شال و رومانف، تنها ژنوتیپ AA را نشان داد. نتایج این مطالعه نشان داد که جایگاه 1PRLR در نژاد شال و آمیخته شال و رومانف چند شکل در حالی که جایگاه 2PRLR در نمونه­های مورد مطالعه یک شکل بود.
متن کامل [PDF 406 kb]   (303 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: تخصصي
دریافت: ۱۳۹۶/۱۲/۹ | ویرایش نهایی: ۱۳۹۶/۱۲/۱۲ | پذیرش: ۱۳۹۶/۱۲/۹ | انتشار: ۱۳۹۶/۱۲/۹

فهرست منابع
1. Alinaghizadeh, H., M.R. Mohammad Abadi and S. Zakizadeh. 2010. Exon 2 of BMP15 gene polymorphisminJabalBarez Red Goat. Journal of Agricultural Biotechnology, 2(1): 69-80 (In Persian).
2. Amiri, F., S.R. Miraei Ashtiani and M. Sadeghi. 2011. Polymorphism in the GDF8 gene and their effects on carcass traits in lori- bakhtiari and zel sheep breeds, 2(4): 12-23.
3. Brym, P., S. Kaminski and E. Wojcik. 2005. Polymorphism within the bovine prolactin receptor gene (PRLR). Animal Science Papers and Reports, 23: 61-66.
4. Chu, M.X., Y.L. Mu, L. Fang and S.C. Ye. 2007. Prolactin receptor as a candidate gene for prolificacy of small tail han sheep. Animal Biotechnology, 18: 65-73. [DOI:10.1080/10495390601090950]
5. Gao, W., X.Y. Lv, X.N. Zhang, Q.Z. Wang, H. Hassan Musa, H.S. Xu, W.H. Hua, Q. Liu, W.Z. Liu and W. Sun. 2015. Association between genetic polymorphsim of exon 10 of prolactin receptor gene and litter size of sheep. Journal of Science, 5(12): 1325-1331.
6. Hadizadeh, M., M.R. Mohammadbadi, A. Niazi, A.K. Esmailizadeh and Y.M. Gazooei. 2014. Search for polymorphism in growth and differentiation factor 9 (GDF9) gene in prolific beetal and tali goats (Capra hircus). Journal of Biodiversity and Environmental Sciences, 4(4): 186-191.
7. Hadizadeh, M., M.R. Mohammadabadi, A. Niazi, A.K. Esmailizadeh, Y.G. Mehdizadeh and S. Molaei. 2013. Use of bioinformatics tools to study exon 2 of GDF9 gene in Tali and Beetal goats. Modern Genetics Journal, 8(334): 283-288.
8. Hadizadeh, M., A. Niazi, M. Mohammad Abadi, A. Esmailizadeh Mehdizadeh and Y. Gazooei. 2014. Bioinformatics analysis of the BMP15 exon 2 in Tali and Beetal goats. Modern Genetics, 9(1): 117-120.
9. Hossein-Zadeh, N.G. 2015. Modeling the growth curve of Iranian Shal sheep using non-linear growth models. Small Ruminant Research, 130: 60-66. [DOI:10.1016/j.smallrumres.2015.07.014]
10. Javanmard, A., M.R. Mohammadabadi, G.E. Zarrigabayi, A.A. Gharahedaghi, M.R. Nassiry, A. Javadmansh and N. Asadzadeh. 2008. Polymorphism within the intron region of the bovine leptin gene in Iranian Sarabi cattle (Iranian Bostaurus). Russian Journal of Genetics, 44(4): 495-497. [DOI:10.1134/S1022795408040169]
11. Ji, D., G. Cheng, Y. Li, H. Zhang, H. Guo and M. Cai. 2016. Association of polymorphisms at PRLR gene loci with litter size in Haimen goat. Biochemistry and Molecular Biology, 1: 23-26.
12. Kamjoo, B., G. Rahimi Mianji, M. abdoli, E. Roomi zadeh and K. Ahmadian. 2011. Detection of allelic polymorphisms in prolactin receptor gene and its association with reproduction trait in Baluchi sheep. 7 national biotechnology congress of I.R. Iran (In Persian).
13. Khanahmadi, A., G. Rahimi Mianji, S.H. Hafezian, R. Khataminejad, N. Mamizadeh, S.M. Mosavi. 2016. Effect of polymorphisms in some candidate genes on twinning in cross breeds of Sall and Romanov. Research on Animal Production, 7(14): 187-192. [DOI:10.29252/rap.7.14.192]
14. Khodabakhshzadeh, R., M.R. Mohamadabadi, A.K. Esmailizadeh, H. Moradi Shahrebabak, F. Bordbar and S. Ansari Namin. 2016a. Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Polish Journal of Veterinary Sciences, 19(2): 281-289. [DOI:10.1515/pjvs-2016-0035]
15. Khodabakhshzadeh, R., M.R. Mohammadabadi, A.K. Esmailizadeh, A. Koshkoieh, H. Moradi-Shahrebabak and S. Ansari Namin. 2015a. Study of mutations available in first-halfexon 2 of GDF9 gene in crossbred sheep born from crossing of Romanov rams with Kermani ewes. Iranian Journal of Animal Science Research, 6(4): 395-403.
16. Khodabakhshzadeh, R., M.R. Mohammadabadi, H. Moradi and A.K. Esmailizadeh. 2016b. Identification of available mutations in the first-half (from 5'end) of exon 2 of GDF9 gene in crossbred sheep from crossing of Romanov and Lori-Bakhtiari breeds. Animal Production Research, 4(4): 15-26.
17. Khodabakhshzadeh, R., M.R. Mohammadabadi, H. Moradi, A.K. Esmailizadeh and S. Ansari Namin 2015b. Identify of G-›A point mutation at positions 477 and 721 in exon 2 of GDF9 gene in Kermani sheep. Modern Genetics, 10(2): 261-268. [DOI:10.1515/pjvs-2016-0035]
18. Kuchtík, J., D. Zapletal and K. Sustova. 2012. Chemical and physical characteristics of lamb meat related to crossbreeding of Romanov ewes with Suffolk and Charollais sires. Meat Science, 90: 426-430. [DOI:10.1016/j.meatsci.2011.08.012]
19. Lu, A., X. Hu, H. Chen, Y. Dong and Y. Pang. 2011. Single nucleotide polymorphisms of the prolactin receptor (PRLR) gene and its association with growth traits in chinese cattle. Molecular Biology Reports, 38: 261-266. [DOI:10.1007/s11033-010-0103-5]
20. Mishra, C. 2014. Genetic Basis of Prolificacy in Sheep. International Journal of Livestock Research, 4(1): 46-57. [DOI:10.5455/ijlr.20131227083421]
21. Moghadaszadeh, M., M.R. Mohammadabadi and A.K. Esmailizadeh. 2015. Association of Exon 2 of BMP15 Gene with the Litter Size in the Raini Cashmere Goat. Genetics in the 3rd Millennium, 13(3): 4062-4067.
22. Mohammadabadi, M.R. and R. Sattayimokhtari. 2013. Estimation of (co) variance components of ewe productivity traits in kermani sheep. Slovak Journal of Animal Science, 46: 45-51.
23. Mohammadi, A., M.R. Nassiry, J. Mosafer, M.R. Mohammadabadi and G.E. Sulimova. 2009. Distribution of BoLA-DRB3 allelic frequencies and identification of a new allele in the Iranian cattle breed Sistani (Bosindicus). Russian journal of genetics, 45(2): 198-202. [DOI:10.1134/S1022795409020100]
24. Mousavizadeh, A., M.R. Mohammad Abadi, A. Torabi, M.R. Nassiry and G.A.K. Esmailizadeh. 2009. Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iranian Journal of Biotechnology, 7(1): 51-53.
25. Notter, D.R. 2012. Genetic improvement of reproductive efficiency of sheep and goats. Animal Reproduction Science, 130: 147-151. [DOI:10.1016/j.anireprosci.2012.01.008]
26. Ozmen, O., I. Seker, O. Ertugrul, E. Ozkan and N. Tekin. 2011. Prolactin receptor (PRLR) gene polymorphism in Chios, White Karaman and Awassi sheep breeds. Archiv Tierzucht, 54(4): 381-390. [DOI:10.5194/aab-54-381-2011]
27. Psifidi, A., C.I. Dovas, G. Bramis, T. Lazou, C.L. Russel, G. Arsenos and G. Banos. 2015. Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples. PLoS One, 10: 0115960. [DOI:10.1371/journal.pone.0115960]
28. Sattai Mokhtari, M., A. Rashidi, M.R. Mohammadabadi and H. Moradishahrbabak. 2009. Estimation of Genetic, Phenotypic and Environmental Trends of Growth Traits in Kermani Sheep. Iranian Journal of Animal Science, 4(1): 51-58.
29. Shamsalddini, S., M.R. Mohammadabadi and A.K. Esmailizadeh. 2016. Polymorphism of the prolactin gene and its effect on fiber traits in goat. Russian Journal of Genetics, 52(4): 405-408. [DOI:10.1134/S1022795416040098]
30. Shojaei, M., M.R. Mohammad Abadi, M. AsadiFozi, O. Dayani, A. Khezri and M. Akhondi. 2010. Association of growth trait and Leptin gene polymorphism in Kermani sheep. Journal of Cell and Molecular Research, 2: 67-73.
31. Soufy, B., M.R. Mohammadabadi, K. Shojaeyan, A. Baghizadeh, S. Ferasaty, N. Askari and O. Dayani. 2009. Evaluation of Myostatin gene polymorphism in Sanjabi sheep by PCR-RFLP method. Animal Science Reserches, 19(1): 81-89.
32. Wang, L.P., R.Q. Geng, X.N. Zhang and W. Sun. 2015. Identification of SNPs within the PRLR gene and effects on maternal behavior in sheep. Genetics and Molecular Research, 14(4): 17536-17543. [DOI:10.4238/2015.December.21.26]
33. Zamani, P., M. Akhondi, M.R. Mohammadabadi, A.A. Saki, A. Ershadi, M.H. Banabazi and A.R. Abdolmohammadi. 2013. Genetic variation of Mehraban sheep using two intersimple sequence repeat (ISSR) markers. African Journal of Biotechnology, 10: 1812-1817.
34. Zhang, C., Y. Wang, H. Chen, X. Lan and C. Lei. 2007. Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining. Analytical Biochemistry, 15(2): 286-287. [DOI:10.1016/j.ab.2007.03.023]