The present study was conducted to evaluate the effect of L-carnitine supplementation accompanied with two sources of fat supplemented diets (ω3 and ω6) on flushing period of Farahani ewes. 20 Farahani ewes were allocated in four different treatments which were as follow: 1) 2.5% dry matter intake ω3 source without L-carnitine 2) 2.5% dry matter intake ω6 source without L-carnitine 3) 2.5% dry matter intake ω3 source with 500 mg L-carnitine 4) 2.5% dry matter intake ω6 source with 500 mg L-carnitine. The experiment was conducted as completely randomized design and the study was lasted 42 days. Some blood metabolites were studied and fertility parameters were considered as well. The results of the present study showed that blood metabolites concentrations such as glucose, cholesterol, hepatics enzymes (AST, ALT), HDL lipoprotein and insulin were not significantly different among the treatments (P<0.05). However serum triglyceride and LDL lipoprotein showed to have a tendency to be significant (P≤0.1). Serum progesterone concentration was affected by treatments (P<0.05). Lamb birth weights were not significantly different among treatments. According to the obtained results sources of omega 3 and 6 had positive impact but the accompanying of L-carnitine with omega-6 showed to have more positive effect on ewes in flushing period.