1- Islamic Azad University-Sanandaj Branch
2- University of Tehran
3- university of Tehran
2- University of Tehran
3- university of Tehran
Abstract: (131 Views)
Extended Abstract
Introduction and Objectives: Leptospirosis is considered one of the most important zoonotic diseases that can cause a wide range of clinical manifestations from subacute to acute forms. The disease is associated with complications such as infertility, abortion, and reduced production performance, leading to significant economic losses in the livestock industry. Laboratory diagnosis of this disease can be performed using various methods including direct microscopic observation, bacterial culture, polymerase chain reaction (PCR), microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assay (IFA). The objective of this study was to compare three diagnostic methods: serology (MAT), urine culture, and PCR method for detecting leptospirosis in infected cattle.
Materials and Methods: In this cross-sectional descriptive study, blood and urine samples were collected from 400 cattle showing at least one clinical sign suggestive of leptospirosis. Blood samples were obtained from the jugular vein following standard hygienic procedures. Serum samples were used for serological testing (MAT), while urine samples were used for bacterial culture and PCR testing.
Results: The results showed that the MAT test was positive in 5 cattle (1.2%). The predominant identified serovars were Icterohaemorrhagiae (2 cases, 40%), Grippotyphosa (2 cases, 40%), and Canicola (1 case, 20%). All MAT-positive samples along with all clinically symptomatic cases (totaling 5 samples) were selected for PCR testing, of which 4 cases (1%) showed positive results. Urine culture was performed for all suspected cases (those with clinical signs and/or positive tests). Although two cultures were contaminated, no growth of Leptospira bacteria was observed in any of the culture media (0%).
Conclusion: The findings of this study indicate a much lower prevalence of leptospirosis in cattle in Markazi Province compared to other studies, which limited the precise comparison between the three diagnostic methods. The notable discrepancy between positive results in MAT and PCR tests highlights the need for further research in this area. Given the technical complexities, time-consuming nature, and high sensitivity requirements of the culture method, it appears that this method may not be practical for routine diagnostic purposes.
Introduction and Objectives: Leptospirosis is considered one of the most important zoonotic diseases that can cause a wide range of clinical manifestations from subacute to acute forms. The disease is associated with complications such as infertility, abortion, and reduced production performance, leading to significant economic losses in the livestock industry. Laboratory diagnosis of this disease can be performed using various methods including direct microscopic observation, bacterial culture, polymerase chain reaction (PCR), microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assay (IFA). The objective of this study was to compare three diagnostic methods: serology (MAT), urine culture, and PCR method for detecting leptospirosis in infected cattle.
Materials and Methods: In this cross-sectional descriptive study, blood and urine samples were collected from 400 cattle showing at least one clinical sign suggestive of leptospirosis. Blood samples were obtained from the jugular vein following standard hygienic procedures. Serum samples were used for serological testing (MAT), while urine samples were used for bacterial culture and PCR testing.
Results: The results showed that the MAT test was positive in 5 cattle (1.2%). The predominant identified serovars were Icterohaemorrhagiae (2 cases, 40%), Grippotyphosa (2 cases, 40%), and Canicola (1 case, 20%). All MAT-positive samples along with all clinically symptomatic cases (totaling 5 samples) were selected for PCR testing, of which 4 cases (1%) showed positive results. Urine culture was performed for all suspected cases (those with clinical signs and/or positive tests). Although two cultures were contaminated, no growth of Leptospira bacteria was observed in any of the culture media (0%).
Conclusion: The findings of this study indicate a much lower prevalence of leptospirosis in cattle in Markazi Province compared to other studies, which limited the precise comparison between the three diagnostic methods. The notable discrepancy between positive results in MAT and PCR tests highlights the need for further research in this area. Given the technical complexities, time-consuming nature, and high sensitivity requirements of the culture method, it appears that this method may not be practical for routine diagnostic purposes.
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