Volume 1, Issue 2 (4-2010)
Res Anim Prod 2010, 1(2): 37-44 |
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Mostafa Sadeghi *1, Mohammad Moradi Shahr Babak1, Ghodratollah Rahimi Mianji2, Ardeshir Nejati Javaremi1
1- Faculty of Agricultural Science and Engineering, Agriculture and Natural Resources University of Tehran
2- College of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University
2- College of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University
Abstract: (7622 Views)
Signal transducer and activator of transcription 5A (STAT5A) was chosen as candidate gene because of its involvement in the development of the mammary gland and it is a key mediator of prolactin signaling and can activate transcription of milk protein genes in response to prolactin. Semen samples from 134 Iranian Holstein proven bulls were obtained from the Animal Breeding Center of Iran. A 215 bp fragment, located within exon 7 of bovine STAT5A gene was amplified. PCR product was digested with AvaI enzyme. Genotypic frequencies of CC and TC were 0.739 and 0.261 respectively and gene frequencies of T and C alleles were 0.131 and 0.869 respectively. Genotypes are distributed according to the Hardy-Weinberg equilibrium. Breeding values for milk related traits (milk, fat and protein yield and percent age) were estimated using animal model and AIREML procedures as programmed in MATVEC software. Least squares means between two genotypes for protein yield were significance (P<0.016) and TC genotype had higher protein yield. We can conclude that RFLP in STAT5A is a promising new possibility to select for increased yield of milk protein through selection for the T allele.
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