Volume 12, Issue 32 (7-2021)                   Res Anim Prod 2021, 12(32): 88-95 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Veshkini A, Kouhkan F, Assadi Alamouti A, Salehi A, Mohammadi-sangcheshmeh A. (2021). The Inhibitory Effect of Fish Oil on Mesenchymal Stem Cells Differentiation to Adipocytes in Sheep. Res Anim Prod. 12(32), 88-95. doi:10.52547/rap.12.32.88
URL: http://rap.sanru.ac.ir/article-1-1139-en.html
University of Tehran
Abstract:   (2313 Views)
    In this study, the expression of genes and miRNAs related to differentiation, cell cycle and metabolism was investigated in Zandi sheep mesenchymal cells (ADSC) under fish oil treatment as a source of omega-3 fatty acids. Isolated sheep mesenchymal stem cells were confirmed for pluripotency. ADSCs were treated in the presence or absence of 15 mg / ml fish oil, and cell cycle and apoptosis induction were measured in each group (three independent repeat) via Flow cytometry. In a similar experiment, the expression of downstream miRNAs and genes at the growth and differentiation stages to adipocyte was compared in both groups. Mesenchymal cells treated with fish oil had a higher percentage of late apoptosis, while the percentage of cells in S phase decreased. The expression of miRNAs did not change significantly, however, let-7a was significantly decreased in the fish oil group. Fish oil treatment decreased (0.39) relative expression of peroxisome proliferator-activated receptor G (PPARG) and increased (1.85) Interleukin 1 beta expression. In adipocyte-differentiated cells, PPARG expression was decreased. The results of this study showed the inhibitory properties of fish oil on adipocyte differentiation of mesenchymal cells through direct or indirect effects on PPAR pathway. However, no significant difference was observed in the expression of selected miRNAs.
Full-Text [PDF 1306 kb]   (629 Downloads)    
Type of Study: Research | Subject: فیزیولوژی
Received: 2020/09/26 | Revised: 2021/08/31 | Accepted: 2020/12/31 | Published: 2021/08/31

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Research On Animal Production

Designed & Developed by : Yektaweb