دوره 12، شماره 31 - ( بهار 1400 )
جلد 12 شماره 31 صفحات 156-146 |
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Enayati K, Farhadi A, Rahimi-Mianji G. (2021). Detection of Lack of Function Mutation of p.Q879X Affecting Abortion in APAF1 Gene in Holstein Cattle. rap. 12(31), 146-156. doi:10.52547/rap.12.31.146
URL: http://rap.sanru.ac.ir/article-1-1120-fa.html
URL: http://rap.sanru.ac.ir/article-1-1120-fa.html
عنایتی خزان، فرهادی ایوب، رحیمی میانجی قدرت. شناسایی جهش فقدان عملکرد p.Q579X موثر بر سقط جنین در ژن APAF1در گاوهای هلشتاین پژوهشهاي توليدات دامي 1400; 12 (31) :156-146 10.52547/rap.12.31.146
گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری
چکیده: (1781 مشاهده)
امروزه مشکلات تولید مثلی یکی از نگرانیهای اصلی در صنعت گاو شیری است. هدف از پژوهش حاضر شناسایی جهش فقدان عملکرد p.Q579X موثر بر سقط جنین خود به خودی و مشکلات تولید مثلی در ژن APAF1 با روش توالی یابی در گاو هلشتاین بود. در مجموع 200 نمونه خون تهیه و استخراج DNA با کیت تجاری انجام شد. سپس یک جفت آغازگر اختصاصی برای تکثیر قطعه ای با طول 456 جفت باز حاوی جهش مورد نظر طراحی و تعیین ژنوتیپ نمونه ها با روش PCR-SSCP انجام شد. در جایگاه APAF1 دو آلل A و B و دو ژنوتیپ AA و AB به ترتیب با فراوانی های 96/76، 3/24، 93/51 و 6/49 درصد مشاهده شدند. ژنوتیپ BB در نمونه های مورد مطالعه مشاهده نشد. بعد از تعیین ژنوتیپ نمونه ها، از هر ژنوتیپ دو نمونه به صورت دو طرفه توالییابیشده و بعد از همترازی توالی های به دست آمده، مقایسه آنها با توالی رفرنس برای شناسایی جهش های مورد نظر با استفاده از نرم افزار BioEdit (version7.0.9.0 ) صورت گرفت. نتایج بررسی های بیوانفورماتیک نشان داد که جهش p.Q579X که یکی از جهش های موثر بر سقط جنین در گاو هلشتاین می باشد در ژنوتیپ AB ژن APAF1 در گاوهای هلشتاین در ایران وجود دارد. با توجه به اینکه این جهش در حالت هموزیگوت باعث سقط جنین در گاو میشود، می توان نتایج حاصل از این پژوهش را در برنامه های اصلاح نژادی در راستای شناسایی حاملین و حذف آنها از گله گاوهای شیری هلشتاین به کار برد.
نوع مطالعه: پژوهشي |
موضوع مقاله:
ژنتیک و اصلاح نژاد دام
دریافت: 1399/4/4 | ویرایش نهایی: 1400/3/25 | پذیرش: 1399/5/13 | انتشار: 1400/3/25
دریافت: 1399/4/4 | ویرایش نهایی: 1400/3/25 | پذیرش: 1399/5/13 | انتشار: 1400/3/25
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